Gonzalez, C.R.; Matzkin, M.E.; Frungieri, M.B.; Terradas, C.; Ponzio, R.; Puigdomenech, E.; Levalle, O.; Calandra, R.S.; Gonzalez-Calvar, S.I. "Expression of the TGF-beta1 system in human testicular pathologies" (2010) Reproductive Biology and Endocrinology. 8
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Background: In non-obstructive azoospermia, histological patterns of Sertoli cell-only Syndrome (SCO) and hypospermatogenesis (H) are commonly found. In these pathologies, Leydig cell hyperplasia (LCH) is detected in some patients. Since TGF-β1 is involved in cellular proliferation/development, the aim of this work was to analyze the expression of TGF-β1, its receptors TGFBRII, TGFBRI (ALK-1 and ALK-5), and the co-receptor endoglin in human biopsies from patients with idiopathic infertility.Methods: Specific immunostaining of TGF-β1, its receptors TGFBRII, TGFBRI (ALK-1 and ALK-5), co-receptor endoglin and Smads proteins, were carried out in testicular biopsies from normal and infertile men with SCO or H. Gene expression of TGF-β1 system were made in biopsies from infertile patients with semi-quantitative and quantitative PCR.Results: Immunohistochemical studies revealed that TGF-β1 and its specific receptors are present in Leydig cells in biopsies from normal tissue or patients with SCO or H with or without LCH. Smad proteins, which are involved in TGF-β1 signaling, are also detected in both their phosphorylated (activated) and dephosphorylated form in all samples TGF-β1, ALK-1 and endoglin gene expression are stronger in human biopsies with LCH than in those with SCO or H. Neither TGFBRII nor ALK-5 gene expression showed significant differences between pathologies. A significant correlation between ALK-1 and endoglin expression was observed.Conclusions: In conclusion, the high levels of mRNA and protein expression of the TGF-β1 system in patients with LCH, particularly ALK1 and its correlation with endoglin, suggest that these proteins acting in concert might be, at least in part, committed actors in the Leydig cell hyperplasia. © 2010 Gonzalez et al; licensee BioMed Central Ltd.


Documento: Artículo
Título:Expression of the TGF-beta1 system in human testicular pathologies
Autor:Gonzalez, C.R.; Matzkin, M.E.; Frungieri, M.B.; Terradas, C.; Ponzio, R.; Puigdomenech, E.; Levalle, O.; Calandra, R.S.; Gonzalez-Calvar, S.I.
Filiación:Instituto de Biología y Medicina Experimental (CONICET), Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
División Endocrinología, Hospital Durand, Av. Díaz Velez 5044, (1405) Buenos Aires, Argentina
Instituto Medico PREFER, Calle 995 N 2348, San Martín (6000), Argentina
Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 (1121), Buenos Aires, Argentina
Palabras clave:endoglin; messenger RNA; Smad protein; Smad1 protein; Smad2 protein; Smad3 protein; Smad5 protein; transforming growth factor beta receptor 1; transforming growth factor beta receptor 2; transforming growth factor beta1; activin receptor 2; ACVRL1 protein, human; cell surface receptor; ENG protein, human; leukocyte antigen; messenger RNA; protein serine threonine kinase; Smad protein; TGF beta type I receptor; TGF-beta type I receptor; transforming growth factor beta receptor; transforming growth factor beta type II receptor; transforming growth factor beta1; transforming growth factor-beta type II receptor; adult; article; clinical article; gene expression; human; human tissue; hypospermatogenesis; immunopathology; infertility; Leydig cell; male; nucleotide sequence; polymerase chain reaction; Sertoli cell only syndrome; testis disease; biosynthesis; hyperplasia; male infertility; metabolism; middle aged; pathology; testis; Activin Receptors, Type II; Adult; Antigens, CD; Humans; Hyperplasia; Infertility, Male; Leydig Cells; Male; Middle Aged; Protein-Serine-Threonine Kinases; Receptors, Cell Surface; Receptors, Transforming Growth Factor beta; RNA, Messenger; Sertoli Cell-Only Syndrome; Smad Proteins; Testicular Diseases; Testis; Transforming Growth Factor beta1
Título revista:Reproductive Biology and Endocrinology
Título revista abreviado:Reprod. Biol. Endocrinol.
CAS:Smad protein, 62395-38-4; Smad1 protein, 444952-89-0; Smad2 protein, 253862-89-4; Smad3 protein, 237417-78-6, 237417-96-8, 237418-00-7; Smad5 protein, 193228-56-7; ACVRL1 protein, human,; Activin Receptors, Type II,; Antigens, CD; ENG protein, human; Protein-Serine-Threonine Kinases,; RNA, Messenger; Receptors, Cell Surface; Receptors, Transforming Growth Factor beta; Smad Proteins; TGF-beta type I receptor,; Transforming Growth Factor beta1; transforming growth factor-beta type II receptor,


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---------- APA ----------
Gonzalez, C.R., Matzkin, M.E., Frungieri, M.B., Terradas, C., Ponzio, R., Puigdomenech, E., Levalle, O.,..., Gonzalez-Calvar, S.I. (2010) . Expression of the TGF-beta1 system in human testicular pathologies. Reproductive Biology and Endocrinology, 8.
---------- CHICAGO ----------
Gonzalez, C.R., Matzkin, M.E., Frungieri, M.B., Terradas, C., Ponzio, R., Puigdomenech, E., et al. "Expression of the TGF-beta1 system in human testicular pathologies" . Reproductive Biology and Endocrinology 8 (2010).
---------- MLA ----------
Gonzalez, C.R., Matzkin, M.E., Frungieri, M.B., Terradas, C., Ponzio, R., Puigdomenech, E., et al. "Expression of the TGF-beta1 system in human testicular pathologies" . Reproductive Biology and Endocrinology, vol. 8, 2010.
---------- VANCOUVER ----------
Gonzalez, C.R., Matzkin, M.E., Frungieri, M.B., Terradas, C., Ponzio, R., Puigdomenech, E., et al. Expression of the TGF-beta1 system in human testicular pathologies. Reprod. Biol. Endocrinol. 2010;8.