Artículo
Liras, M.; Simoncelli, S.; Rivas-Aravena, A.; García, O.; Scaiano, J.C.; Alarcon, E.I.; Aspée, A. "Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy" (2016) Organic and Biomolecular Chemistry. 14(17):4023-4026
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Abstract:
A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered. © 2016 The Royal Society of Chemistry.
Registro:
| Documento: | Artículo |
| Título: | Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy |
| Autor: | Liras, M.; Simoncelli, S.; Rivas-Aravena, A.; García, O.; Scaiano, J.C.; Alarcon, E.I.; Aspée, A. |
| Filiación: | Instituto de Ciencia y Tecnología de Polímeros, Consejo Superior de Investigaciones Científicas (ICTP-CSIC), Juan de la Cierva 3, Madrid, 28006, Spain Department of Chemistry and Biomolecular Sciences, Centre for Catalysis Research and Innovation, University of Ottawa, 10 Marie Curie, Ottawa, ON K1N6N5, Canada INQUIMAE, Departamento de Química Inorgánica Analítica, y Química Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina Laboratorio de Radiobiología Molecular y Celular, Departamento de Aplicaciones Nucleares, Comisión Chilena de Energía Nuclear, Santiago, Chile Bio-nanomaterials Chemistry and Engineering Laboratory, Division of Cardiac Surgery, University of Ottawa Heart Institute, 40 Ruskin St., Ottawa, ON K1Y 4W7, Canada Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 Correo 33, Santiago, Chile Photoactivated Processes Unit, Instituto IMDEA Energía, Avda. Ramon de la Sagra, 3, Parque Tecnologico de Mostoles, Móstoles, Madrid, E-28935, Spain Photonics and Optoelectronics Group, Department of Physics and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität München, Amalienstraße 54, Munich, 80799, Germany |
| Palabras clave: | Amides; Cell culture; Cytology; Fibroblasts; Fluorescence; Fluorescence microscopy; Cellular redox; Fluorescence intensities; Human fibroblast; Nitroxides; Probes; 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; amide; boron derivative; fluorescent dye; hydrogen peroxide; nitrogen oxide; cell culture; chemistry; fibroblast; fluorescence microscopy; human; metabolism; oxidation reduction reaction; synthesis; Amides; Boron Compounds; Cells, Cultured; Fibroblasts; Fluorescent Dyes; Humans; Hydrogen Peroxide; Microscopy, Fluorescence; Nitrogen Oxides; Oxidation-Reduction |
| Año: | 2016 |
| Volumen: | 14 |
| Número: | 17 |
| Página de inicio: | 4023 |
| Página de fin: | 4026 |
| DOI: | http://dx.doi.org/10.1039/c6ob00533k |
| Título revista: | Organic and Biomolecular Chemistry |
| Título revista abreviado: | Org. Biomol. Chem. |
| ISSN: | 14770520 |
| CODEN: | OBCRA |
| CAS: | amide, 17655-31-1; hydrogen peroxide, 7722-84-1; nitrogen oxide, 11104-93-1; 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; Amides; Boron Compounds; Fluorescent Dyes; Hydrogen Peroxide; Nitrogen Oxides |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14770520_v14_n17_p4023_Liras |
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Citas:
---------- APA ----------
Liras, M., Simoncelli, S., Rivas-Aravena, A., García, O., Scaiano, J.C., Alarcon, E.I. & Aspée, A. (2016) . Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy. Organic and Biomolecular Chemistry, 14(17), 4023-4026.http://dx.doi.org/10.1039/c6ob00533k
---------- CHICAGO ----------
Liras, M., Simoncelli, S., Rivas-Aravena, A., García, O., Scaiano, J.C., Alarcon, E.I., et al. "Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy" . Organic and Biomolecular Chemistry 14, no. 17 (2016) : 4023-4026.http://dx.doi.org/10.1039/c6ob00533k
---------- MLA ----------
Liras, M., Simoncelli, S., Rivas-Aravena, A., García, O., Scaiano, J.C., Alarcon, E.I., et al. "Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy" . Organic and Biomolecular Chemistry, vol. 14, no. 17, 2016, pp. 4023-4026.http://dx.doi.org/10.1039/c6ob00533k
---------- VANCOUVER ----------
Liras, M., Simoncelli, S., Rivas-Aravena, A., García, O., Scaiano, J.C., Alarcon, E.I., et al. Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy. Org. Biomol. Chem. 2016;14(17):4023-4026.http://dx.doi.org/10.1039/c6ob00533k
