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Fukuda, H.; Dopera De Kracoff, Y.E.; Inigo, L.E.; Paredes, S.R.; Ferramola De Sancovich, A.M.; Sancovich, H.A.; Batlle, A.M.C. "Further evidence for an essential histidyl residue at the active site of pig liver 5-aminolevulinic acid dehydratase" (1990) Journal of Enzyme Inhibition and Medicinal Chemistry. 3(4):295-302
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Abstract:

Photoxidation with methylene blue and rose bengal and chemical modification by diethylpyrocarbonate of pig liver 5-aminolevulinic acid dehydratase produced strong inactivation of the enzyme which was concentration dependent. Loss of enzyme activity by both photoxidation and ethoxyformylation was pH and time-dependent and protected by the presence of the substate and competitive inhibitors. The rate of inactivation was directly related to the state of protonation of histidyl groups, the unprotonated from being modified at a much faster rate than the protonated form. Plots of the pseudo-first order rate constants for 5-aminolevulinic acid dehydratase inactivation against pH resulted in typical titration curves showing inflection points at about pH 6.4 for methylene blue and rose bengal and 6.8 for diethylpyrocarbonate providing further and unequivocal evidence for the existence of critical histidyl groups at the active centre of the enzyme. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Registro:

Documento: Artículo
Título:Further evidence for an essential histidyl residue at the active site of pig liver 5-aminolevulinic acid dehydratase
Autor:Fukuda, H.; Dopera De Kracoff, Y.E.; Inigo, L.E.; Paredes, S.R.; Ferramola De Sancovich, A.M.; Sancovich, H.A.; Batlle, A.M.C.
Filiación:Department of Biochemistry, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellhn II 1428, Nuñez, Buenos Aires, Argentina
Palabras clave:5-Aminolevulinic acid dehydratase; Diethylpyrocarbonate; Histidyl residues; Methylene blue; Rose Bengal; diethyl pyrocarbonate; histidine; methylene blue; porphobilinogen synthase; rose bengal; animal cell; article; enzyme active site; enzyme modification; nonhuman; photooxidation; swine; Animal; Binding Sites; Enzyme Inhibitors; Histidine; Hydrogen-Ion Concentration; Kinetics; Liver; Macromolecular Systems; Methylene Blue; Photochemistry; Porphobilinogen Synthase; Rose Bengal; Support, Non-U.S. Gov't; Swine; Animalia; Sus scrofa
Año:1990
Volumen:3
Número:4
Página de inicio:295
Página de fin:302
DOI: http://dx.doi.org/10.3109/14756369009030378
Título revista:Journal of Enzyme Inhibition and Medicinal Chemistry
Título revista abreviado:J. Enzyme Inhib. Med. Chem.
ISSN:14756366
CODEN:JEIMA
CAS:Enzyme Inhibitors; Histidine, 71-00-1; Macromolecular Systems; Methylene Blue, 61-73-4; Porphobilinogen Synthase, EC 4.2.1.24; Rose Bengal, 11121-48-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14756366_v3_n4_p295_Fukuda

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Citas:

---------- APA ----------
Fukuda, H., Dopera De Kracoff, Y.E., Inigo, L.E., Paredes, S.R., Ferramola De Sancovich, A.M., Sancovich, H.A. & Batlle, A.M.C. (1990) . Further evidence for an essential histidyl residue at the active site of pig liver 5-aminolevulinic acid dehydratase. Journal of Enzyme Inhibition and Medicinal Chemistry, 3(4), 295-302.
http://dx.doi.org/10.3109/14756369009030378
---------- CHICAGO ----------
Fukuda, H., Dopera De Kracoff, Y.E., Inigo, L.E., Paredes, S.R., Ferramola De Sancovich, A.M., Sancovich, H.A., et al. "Further evidence for an essential histidyl residue at the active site of pig liver 5-aminolevulinic acid dehydratase" . Journal of Enzyme Inhibition and Medicinal Chemistry 3, no. 4 (1990) : 295-302.
http://dx.doi.org/10.3109/14756369009030378
---------- MLA ----------
Fukuda, H., Dopera De Kracoff, Y.E., Inigo, L.E., Paredes, S.R., Ferramola De Sancovich, A.M., Sancovich, H.A., et al. "Further evidence for an essential histidyl residue at the active site of pig liver 5-aminolevulinic acid dehydratase" . Journal of Enzyme Inhibition and Medicinal Chemistry, vol. 3, no. 4, 1990, pp. 295-302.
http://dx.doi.org/10.3109/14756369009030378
---------- VANCOUVER ----------
Fukuda, H., Dopera De Kracoff, Y.E., Inigo, L.E., Paredes, S.R., Ferramola De Sancovich, A.M., Sancovich, H.A., et al. Further evidence for an essential histidyl residue at the active site of pig liver 5-aminolevulinic acid dehydratase. J. Enzyme Inhib. Med. Chem. 1990;3(4):295-302.
http://dx.doi.org/10.3109/14756369009030378