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Abstract:

Reflectance spectra from pink petals of Rhododendron indicum flowers showed absorption in the NIR (1470, 1930 and 2500 nm) due to water, in the visible (533 nm, due to anthocyanins) and in the UV (broad absorption due to phenolic compounds other than anthocyanins). A linear correlation between the remission function at 533 nm and the anthocyanin content in μmol per g fresh weight has been found, allowing non-destructive quantification of anthocyanins. The remission function could be obtained either from reflectance of a group of stacked petals (Kubelka-Munk theory) or through determination of the absorption and scattering coefficients following the Pile of Plates model. The intact petals have shown fluorescence emission in the blue (400-500 nm) and in the visible around 624 nm under UV excitation. The red emission was attributed to anthocyanins whereas blue emission was assigned to other phenolic compounds. On the basis of absorption and fluorescence measurements of crude and purified extracts from the petals, the last compounds could possibly be a mixture of flavonoids and hydroxycinnamic-type plant phenolics such as ferulic acid, chlorogenic acid or others. © 2009 The Royal Society of Chemistry and Owner Societies.

Registro:

Documento: Artículo
Título:Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method
Autor:Iriel, A.; Lagorio, M.G.
Filiación:INQUIMAE/Dpto. de Química Inorgánica, Analítica y Qca Física, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón II 1er piso, C1428EHA, Buenos Aires, Argentina
Palabras clave:anthocyanin; flavonoid; phenol derivative; absorption; article; flower; fluorescence; nonhuman; petal; priority journal; reflectometry; Rhododendron; spectroscopy; ultraviolet radiation; Anthocyanins; Flavonoids; Flowers; Phenols; Pigments, Biological; Rhododendron; Spectrometry, Fluorescence; Rhododendron indicum
Año:2009
Volumen:8
Número:3
Página de inicio:337
Página de fin:344
DOI: http://dx.doi.org/10.1039/b814461c
Título revista:Photochemical and Photobiological Sciences
Título revista abreviado:Photochem. Photobiol. Sci.
ISSN:1474905X
CODEN:PPSHC
CAS:Anthocyanins; Flavonoids; Phenols; Pigments, Biological
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1474905X_v8_n3_p337_Iriel

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Citas:

---------- APA ----------
Iriel, A. & Lagorio, M.G. (2009) . Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method. Photochemical and Photobiological Sciences, 8(3), 337-344.
http://dx.doi.org/10.1039/b814461c
---------- CHICAGO ----------
Iriel, A., Lagorio, M.G. "Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method" . Photochemical and Photobiological Sciences 8, no. 3 (2009) : 337-344.
http://dx.doi.org/10.1039/b814461c
---------- MLA ----------
Iriel, A., Lagorio, M.G. "Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method" . Photochemical and Photobiological Sciences, vol. 8, no. 3, 2009, pp. 337-344.
http://dx.doi.org/10.1039/b814461c
---------- VANCOUVER ----------
Iriel, A., Lagorio, M.G. Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method. Photochem. Photobiol. Sci. 2009;8(3):337-344.
http://dx.doi.org/10.1039/b814461c