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Abstract:

Chlorophyll-a contained in the peel of Granny Smith apples emits fluorescence upon excitation with blue light. The observed emission, collected by an external detector and corrected by its spectral response, is still distorted by light reabsorption processes taking place in the fruit skin and differs appreciably from the true spectral distribution of fluorescence emerging from chlorophyll molecules in the biological tissue. Reabsorption processes particularly affect the ratio of fluorescence intensities at 680 nm and at 730 nm. A model to obtain the correct spectral distribution of the emission, from the experimental fluorescence recorded at a fluorometer detector and corrected for the detector spectral sensitivity, is developed in the present work. Measurements of the whole fruit reflectance, the peel transmittance and the flesh reflectance allow the calculation of the reabsorption-corrected spectra. The model is validated by comparing the corrected emission spectra with that obtained for a thin layer of apple-peel-chloroplasts, where no reabsorption takes place. It is recommended to correct distortions in emission spectra of intact fruits due to light reabsorption effects whenever a correlation between the physiological state of the fruit and its fluorescence spectra is investigated. © The Royal Society of Chemistry and Owner Societies 2006.

Registro:

Documento: Artículo
Título:A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples
Autor:Ramos, M.E.; Lagorio, M.G.
Filiación:INQUIMAE/Dpto. de Química Inorgánica, Analítica Y Qca. Física, Facultad de Ciencias Exactas Y Naturales, Pabellón II, C1428EHA, Buenos Aires, Argentina
Palabras clave:chlorophyll; apple; article; blue light; chloroplast; correlation analysis; fluorescence; light absorption; measurement; model; nonhuman; photosynthesis; priority journal; spectroscopy; Absorption; Chlorophyll; Fluorescence; Light; Malus; Models, Chemical; Spectrometry, Fluorescence; Malus x domestica
Año:2006
Volumen:5
Número:5
Página de inicio:508
Página de fin:512
DOI: http://dx.doi.org/10.1039/b514248b
Título revista:Photochemical and Photobiological Sciences
Título revista abreviado:Photochem. Photobiol. Sci.
ISSN:1474905X
CODEN:PPSHC
CAS:chlorophyll, 1406-65-1, 15611-43-5; chlorophyll a, 479-61-8; Chlorophyll, 1406-65-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1474905X_v5_n5_p508_Ramos

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Citas:

---------- APA ----------
Ramos, M.E. & Lagorio, M.G. (2006) . A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples. Photochemical and Photobiological Sciences, 5(5), 508-512.
http://dx.doi.org/10.1039/b514248b
---------- CHICAGO ----------
Ramos, M.E., Lagorio, M.G. "A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples" . Photochemical and Photobiological Sciences 5, no. 5 (2006) : 508-512.
http://dx.doi.org/10.1039/b514248b
---------- MLA ----------
Ramos, M.E., Lagorio, M.G. "A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples" . Photochemical and Photobiological Sciences, vol. 5, no. 5, 2006, pp. 508-512.
http://dx.doi.org/10.1039/b514248b
---------- VANCOUVER ----------
Ramos, M.E., Lagorio, M.G. A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples. Photochem. Photobiol. Sci. 2006;5(5):508-512.
http://dx.doi.org/10.1039/b514248b