Conferencia
Abstract:
Fast and easy-to-do methods capable to generate metabolite profile of plant single cells and tissues can be quite beneficial for analyzing biochemical and physiological status of growing crops in plant growth factories. Here we report the applicability of Pressure Probe, which is routinely used for pressure probing of living single cells, for microsampling of plant cells. The picoliter sample of cytoplasm was then analyzed for characterizing metabolite content by MALDI mass spectrometry (MS). This powerful technique could make molecular profile of the cell samples by detecting very small amount of metabolites by using our selected matrixes, after verification of detected signals by MS/MS analysis. Concurrent to single cell sampling and mass spectrometry, the plant tissues, from which single cells were analyzed, were directly profiled by MALDI MS after deposition of our novel nanoparticle matrixes on the dried surface of tissue. MALDI MS showed to be able to detect and verify metabolites, particularly sugars, directly from the surface of plant tissues. Interestingly, metabolites, particularly neutral sugars, detected in cell microsamples were comparable to those detected on the tissue. Accordingly, the single cell and tissue analysis can be concurrently carried out to create metabolite profile, an instant picture of the physiology in cell and tissue levels respectively, of plants during growth without need to common metabolite extraction and purification processes, with minimum preparation and high accuracy and reliability. © 2011 IFAC.
Registro:
| Documento: | Conferencia |
| Título: | Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories |
| Autor: | Nonami, H.; Gholipour, Y.; Erra-Balsells, R. |
| Filiación: | Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, Matsuyama, Japan CIHIDECAR, Departamento de Química Orgánica, Universidad de Buenos Aires, Buenos Aires, Argentina |
| Palabras clave: | Carbon nanotube; In situ profiling; Inorganic nanoparticle; MALDI MS; Pressure probe; Tulip; Biomolecules; Carbon nanotubes; Cells; Cytology; Histology; Mass spectrometry; Metabolites; Nanoparticles; Physiology; Plant cell culture; Plant life extension; Potential flow; Probes; Reliability analysis; Sugars; Tissue; Yarn; Inorganic nanoparticle; MALDI-mass spectrometry; MALDI-MS; Metabolite extraction; Metabolite profiles; Physiological status; Pressure probes; Tulip; Tissue engineering |
| Año: | 2011 |
| Volumen: | 44 |
| Número: | 1 PART 1 |
| Página de inicio: | 1791 |
| Página de fin: | 1795 |
| DOI: | http://dx.doi.org/10.3182/20110828-6-IT-1002.00450 |
| Título revista: | IFAC Proceedings Volumes (IFAC-PapersOnline) |
| Título revista abreviado: | IFAC Proc. Vol. (IFAC-PapersOnline) |
| ISSN: | 14746670 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14746670_v44_n1PART1_p1791_Nonami |
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Citas:
---------- APA ----------
Nonami, H., Gholipour, Y. & Erra-Balsells, R. (2011) . Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories. IFAC Proceedings Volumes (IFAC-PapersOnline), 44(1 PART 1), 1791-1795.http://dx.doi.org/10.3182/20110828-6-IT-1002.00450
---------- CHICAGO ----------
Nonami, H., Gholipour, Y., Erra-Balsells, R. "Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories" . IFAC Proceedings Volumes (IFAC-PapersOnline) 44, no. 1 PART 1 (2011) : 1791-1795.http://dx.doi.org/10.3182/20110828-6-IT-1002.00450
---------- MLA ----------
Nonami, H., Gholipour, Y., Erra-Balsells, R. "Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories" . IFAC Proceedings Volumes (IFAC-PapersOnline), vol. 44, no. 1 PART 1, 2011, pp. 1791-1795.http://dx.doi.org/10.3182/20110828-6-IT-1002.00450
---------- VANCOUVER ----------
Nonami, H., Gholipour, Y., Erra-Balsells, R. Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories. IFAC Proc. Vol. (IFAC-PapersOnline). 2011;44(1 PART 1):1791-1795.http://dx.doi.org/10.3182/20110828-6-IT-1002.00450
