Artículo

Ricardi, M.M.; González, R.M.; Zhong, S.; Domínguez, P.G.; Duffy, T.; Turjanski, P.G.; Salgado Salter, J.D.; Alleva, K.; Carrari, F.; Giovannoni, J.J.; Estévez, J.M.; Iusem, N.D. "Genome-wide data (ChIP-seq) enabled identification of cell wall-related and aquaporin genes as targets of tomato ASR1, a drought stress-responsive transcription factor" (2014) BMC Plant Biology. 14(1)
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Abstract:

Background: Identifying the target genes of transcription factors is important for unraveling regulatory networks in all types of organisms. Our interest was precisely to uncover the spectrum of loci regulated by a widespread plant transcription factor involved in physiological adaptation to drought, a type of stress that plants have encountered since the colonization of land habitats 400 MYA. The regulator under study, named ASR1, is exclusive to the plant kingdom (albeit absent in Arabidopsis) and known to alleviate the stress caused by restricted water availability. As its target genes are still unknown despite the original cloning of Asr1 cDNA 20 years ago, we examined the tomato genome for specific loci interacting in vivo with this conspicuous protein. Results: We performed ChIP followed by high throughput DNA sequencing (ChIP-seq) on leaves from stressed tomato plants, using a high-quality anti-ASR1 antibody. In this way, we unraveled a novel repertoire of target genes, some of which are clearly involved in the response to drought stress. Many of the ASR1-enriched genomic loci we found encode enzymes involved in cell wall synthesis and remodeling as well as channels implicated in water and solute flux, such as aquaporins. In addition, we were able to determine a robust consensus ASR1-binding DNA motif.Conclusions: The finding of cell wall synthesis and aquaporin genes as targets of ASR1 is consistent with their suggested role in the physiological adaptation of plants to water loss. The results gain insight into the environmental stress-sensing pathways leading to plant tolerance of drought. © 2014 Ricardi et al.; licensee BioMed Central Ltd.

Registro:

Documento: Artículo
Título:Genome-wide data (ChIP-seq) enabled identification of cell wall-related and aquaporin genes as targets of tomato ASR1, a drought stress-responsive transcription factor
Autor:Ricardi, M.M.; González, R.M.; Zhong, S.; Domínguez, P.G.; Duffy, T.; Turjanski, P.G.; Salgado Salter, J.D.; Alleva, K.; Carrari, F.; Giovannoni, J.J.; Estévez, J.M.; Iusem, N.D.
Filiación:Instituto de Fisiología, Biología Molecular y Neurociencias (IFIByNE)-CONICET, Buenos Aires, Argentina
Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY, United States
Instituto de Biotecnología - INTA, Hurlingham, Provincia de Buenos Aires, Argentina
Departamento de Computación, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Biodiversidad y Biología Experimental (IBBEA, CONICET-UBA), Buenos Aires, Argentina
Department of Plant Biology, Cornell University, Ithaca, NY, United States
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Aquaporin; ASR1; Cell wall; ChIP-seq; Tomato; Water stress; Arabidopsis; Lycopersicon esculentum; Arabidopsis; Lycopersicon esculentum; aquaporin; transcription factor; vegetable protein; article; cell wall; chromatin immunoprecipitation; gene expression regulation; genetics; metabolism; tomato; Aquaporins; Cell Wall; Chromatin Immunoprecipitation; Gene Expression Regulation, Plant; Lycopersicon esculentum; Plant Proteins; Transcription Factors
Año:2014
Volumen:14
Número:1
DOI: http://dx.doi.org/10.1186/1471-2229-14-29
Título revista:BMC Plant Biology
Título revista abreviado:BMC Plant Biol.
ISSN:14712229
CAS:aquaporin, 215587-75-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712229_v14_n1_p_Ricardi

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Citas:

---------- APA ----------
Ricardi, M.M., González, R.M., Zhong, S., Domínguez, P.G., Duffy, T., Turjanski, P.G., Salgado Salter, J.D.,..., Iusem, N.D. (2014) . Genome-wide data (ChIP-seq) enabled identification of cell wall-related and aquaporin genes as targets of tomato ASR1, a drought stress-responsive transcription factor. BMC Plant Biology, 14(1).
http://dx.doi.org/10.1186/1471-2229-14-29
---------- CHICAGO ----------
Ricardi, M.M., González, R.M., Zhong, S., Domínguez, P.G., Duffy, T., Turjanski, P.G., et al. "Genome-wide data (ChIP-seq) enabled identification of cell wall-related and aquaporin genes as targets of tomato ASR1, a drought stress-responsive transcription factor" . BMC Plant Biology 14, no. 1 (2014).
http://dx.doi.org/10.1186/1471-2229-14-29
---------- MLA ----------
Ricardi, M.M., González, R.M., Zhong, S., Domínguez, P.G., Duffy, T., Turjanski, P.G., et al. "Genome-wide data (ChIP-seq) enabled identification of cell wall-related and aquaporin genes as targets of tomato ASR1, a drought stress-responsive transcription factor" . BMC Plant Biology, vol. 14, no. 1, 2014.
http://dx.doi.org/10.1186/1471-2229-14-29
---------- VANCOUVER ----------
Ricardi, M.M., González, R.M., Zhong, S., Domínguez, P.G., Duffy, T., Turjanski, P.G., et al. Genome-wide data (ChIP-seq) enabled identification of cell wall-related and aquaporin genes as targets of tomato ASR1, a drought stress-responsive transcription factor. BMC Plant Biol. 2014;14(1).
http://dx.doi.org/10.1186/1471-2229-14-29