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Abstract:

Background: Among neotropical Primates, the Cai monkey Cebus paraguayanus (CPA) presents long, conserved chromosome syntenies with the human karyotype (HSA) as well as numerous C+ blocks in different chromosome pairs. In this study, immunofluorescence (IF) against two proteins of the Synaptonemal Complex (SC), namely REC8 and SYCP1, two recombination protein markers (RPA and MLH1), and one protein involved in the pachytene checkpoint machinery (BRCA1) was performed in CPA spermatocytes in order to analyze chromosome meiotic behavior in detail. Results: Although in the vast majority of pachytene cells all autosomeswere paired and synapsed, in a small number of nuclei the heterochromatic C-positive terminal region of bivalent 11 remained unpaired. The analysis of 75 CPA cells at pachytene revealed a mean of 43.22 MLH1 foci per nucleus and 1.07 MLH1 foci in each CPA bivalent 11, always positioned in the region homologous to HSA chromosome 21. Conclusion: Our results suggest that C blocks undergo delayed pairing and synapsis, although they do not interfere with the general progress of pairing and synapsis. © 2009 García-Cruz et al; licensee BioMed Central Ltd.

Registro:

Documento: Artículo
Título:Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini)
Autor:Garcia-Cruz, R.; Robles, P.; Steinberg, E.R.; Camats, N.; Brieño, M.A.; Garcia-Caldés, M.; Mudry, M.D.
Filiación:Unitat de Biologia Cellular i Genètica Mèdica, Facultat de Medicina, Universitat Autònoma de Barcelona, Barcelona, Spain
CONICET, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
Grupo de Investigación en Biología Evolutiva (GIBE), Departamento de Ecología, Genética y Evolución, UBA, Buenos Aires, Argentina
Palabras clave:BRCA1 protein; chromosome protein; protein MLH1; protein Rec8; protein SYCPI; replication factor A; unclassified drug; cell cycle protein; animal cell; article; autosome; Cebus paraguayanus; cell nucleus; chromosome bivalent; chromosome pairing; genetic recombination; heterochromatin; immunofluorescence; meiosis; nonhuman; pachytene; primate; protein structure; animal; Cebidae; chromosome; fluorescence in situ hybridization; genetics; male; metabolism; spermatocyte; Cebus; Platyrrhini; Primates; Synapsis; Animals; Cebus; Cell Cycle Proteins; Chromosome Pairing; Chromosomes; Heterochromatin; In Situ Hybridization, Fluorescence; Male; Meiosis; Recombination, Genetic; Spermatocytes
Año:2009
Volumen:10
DOI: http://dx.doi.org/10.1186/1471-2156-10-25
Título revista:BMC Genetics
Título revista abreviado:BMC Genet.
ISSN:14712156
CODEN:BGMED
CAS:protein MLH1, 155577-96-1; Cell Cycle Proteins; Heterochromatin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712156_v10_n_p_GarciaCruz

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Citas:

---------- APA ----------
Garcia-Cruz, R., Robles, P., Steinberg, E.R., Camats, N., Brieño, M.A., Garcia-Caldés, M. & Mudry, M.D. (2009) . Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini). BMC Genetics, 10.
http://dx.doi.org/10.1186/1471-2156-10-25
---------- CHICAGO ----------
Garcia-Cruz, R., Robles, P., Steinberg, E.R., Camats, N., Brieño, M.A., Garcia-Caldés, M., et al. "Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini)" . BMC Genetics 10 (2009).
http://dx.doi.org/10.1186/1471-2156-10-25
---------- MLA ----------
Garcia-Cruz, R., Robles, P., Steinberg, E.R., Camats, N., Brieño, M.A., Garcia-Caldés, M., et al. "Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini)" . BMC Genetics, vol. 10, 2009.
http://dx.doi.org/10.1186/1471-2156-10-25
---------- VANCOUVER ----------
Garcia-Cruz, R., Robles, P., Steinberg, E.R., Camats, N., Brieño, M.A., Garcia-Caldés, M., et al. Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini). BMC Genet. 2009;10.
http://dx.doi.org/10.1186/1471-2156-10-25