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Vicent, G.P.; Lanuza, G.M.; Romero, D.G.; Di Croce, L.; Pecci, A. "Glucocorticoid-induced apoptosis: A simple set of laboratory experiments" (2000) Biochemistry and Molecular Biology Education. 28(6):307-312
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Abstract:

Here, we present a laboratory experiment for students of human biology and medicine. In this experiment the students have the opportunity of measuring one of the hallmarks of programmed cell death, which is DNA fragmentation. The biological system that we examine here is the glucocorticoid-induced apoptosis in the thymus. The set of experiments that we propose to perform and discuss are relatively simple and can be rapidly carried out in a course for undergraduates. This practice also allows the students to familiarize themselves with basic pharmacology, compare in vivo vs. in vitro assays and acquire skills in animal handling. The organisation of the practical work requires 4-5h of experimental training in each of two days. Copyright © 2001 IUBMB.

Registro:

Documento: Artículo
Título:Glucocorticoid-induced apoptosis: A simple set of laboratory experiments
Autor:Vicent, G.P.; Lanuza, G.M.; Romero, D.G.; Di Croce, L.; Pecci, A.
Filiación:Departamento De Química Biológica, Facultad De Ciencias Exactas Y Naturales, Universidad De Buenos Aires Y PRHOM (CONICET), Pabellón II Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:corticosterone; dexamethasone; DNA fragment; glucocorticoid; glucocorticoid receptor; mifepristone; progesterone; testosterone; animal cell; apoptosis; concentration response; conference paper; drug effect; male; nonhuman; rat; thymocyte; thymus; Animalia
Año:2000
Volumen:28
Número:6
Página de inicio:307
Página de fin:312
DOI: http://dx.doi.org/10.1016/S1470-8175(00)00025-4
Título revista:Biochemistry and Molecular Biology Education
Título revista abreviado:Biochem. Mol. Biol. Educ.
ISSN:14708175
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14708175_v28_n6_p307_Vicent

Referencias:

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Citas:

---------- APA ----------
Vicent, G.P., Lanuza, G.M., Romero, D.G., Di Croce, L. & Pecci, A. (2000) . Glucocorticoid-induced apoptosis: A simple set of laboratory experiments. Biochemistry and Molecular Biology Education, 28(6), 307-312.
http://dx.doi.org/10.1016/S1470-8175(00)00025-4
---------- CHICAGO ----------
Vicent, G.P., Lanuza, G.M., Romero, D.G., Di Croce, L., Pecci, A. "Glucocorticoid-induced apoptosis: A simple set of laboratory experiments" . Biochemistry and Molecular Biology Education 28, no. 6 (2000) : 307-312.
http://dx.doi.org/10.1016/S1470-8175(00)00025-4
---------- MLA ----------
Vicent, G.P., Lanuza, G.M., Romero, D.G., Di Croce, L., Pecci, A. "Glucocorticoid-induced apoptosis: A simple set of laboratory experiments" . Biochemistry and Molecular Biology Education, vol. 28, no. 6, 2000, pp. 307-312.
http://dx.doi.org/10.1016/S1470-8175(00)00025-4
---------- VANCOUVER ----------
Vicent, G.P., Lanuza, G.M., Romero, D.G., Di Croce, L., Pecci, A. Glucocorticoid-induced apoptosis: A simple set of laboratory experiments. Biochem. Mol. Biol. Educ. 2000;28(6):307-312.
http://dx.doi.org/10.1016/S1470-8175(00)00025-4