Artículo

Sánchez, M.C.; Fontana, V.A.; Galotto, C.; Cambiasso, M.Y.; Sobarzo, C.M.A.; Calvo, L.; Calvo, J.C.; Cebral, E. "Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake" (2018) Reproduction. 155(6):529-541
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Abstract:

Male chronic alcohol abuse causes testicular failure and infertility. We analyzed the effects of moderate sub-chronic alcohol intake on sperm morphology, capacitation, fertilization and sperm head decondensation. CF-1 male mice were administered 15% ethanol in drinking water for 15 days; control mice received ethanol-free water. Similar patterns of tyrosine phosphorylation were observed in capacitated spermatozoa of control and treated males. Percentage of hyperactivation (H) and spontaneous (SAR) and progesterone-induced (IAR) acrosome reaction significantly decreased at 120 and 150min of capacitation in treated males compared to controls (H: 14.1±2.5 vs 23.7±2.6, P<0.05; SAR-T120min: 17.9±2.5 vs 32.9±4.1, P<0.01; IAR-150min: 43.3±3.5 vs 73.1±1.1, P<0.001, n=6). During in vitro fertilization (2.5, 3.5 and 4.5h post-insemination), there was an increased percentage of fertilized oocytes (with a decondensed sperm head and one or two pronuclei) in treated males (P<0.001, n=7). After 60min of in vitro decondensation with glutathione plus heparin, the percentage of decondensed sperm heads was significantly higher in treated males than in controls (mean±s.d.: 57.1±5.6 vs 48.3±4.5, P<0.05, n=5). The percentage of morphologically normal sperm heads was significantly decreased in treated males with respect to controls (P<0.001, n=9). These results show that short-term moderate alcohol consumption in outbred mice affect sperm morphology, hyperactivation, acrosomal exocytosis, and the dynamics of in vitro fertilization and in vitro sperm nuclear decondensation. © 2018 Society for Reproduction and Fertility.

Registro:

Documento: Artículo
Título:Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake
Autor:Sánchez, M.C.; Fontana, V.A.; Galotto, C.; Cambiasso, M.Y.; Sobarzo, C.M.A.; Calvo, L.; Calvo, J.C.; Cebral, E.
Filiación:CONICET, Instituto de Biología y Medicina Experimental (IByME), Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Medicina, CONICET-Universidad de Buenos Aires, Instituto de Investigaciones Biomédicas (INBIOMED), Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
CONICET, Universidad de Buenos Aires, Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA-CONICET), Buenos Aires, Argentina
Palabras clave:alcohol; progesterone; tyrosine; alcohol; topical antiinfective agent; acrosome reaction; alcohol consumption; animal cell; animal experiment; Article; controlled study; exocytosis; in vitro fertilization; in vitro study; male; mouse; nonhuman; oocyte; oocyte penetration; priority journal; protein phosphorylation; reproduction; spermatozoon capacitation; spermatozoon head; spermatozoon motility; animal; drug effect; fertility; pathology; spermatozoon capacitation; Acrosome Reaction; Animals; Anti-Infective Agents, Local; Ethanol; Fertilization in Vitro; Male; Mice; Oocytes; Sperm Capacitation; Sperm-Ovum Interactions
Año:2018
Volumen:155
Número:6
Página de inicio:529
Página de fin:541
DOI: http://dx.doi.org/10.1530/REP-17-0507
Título revista:Reproduction
Título revista abreviado:Reproduction
ISSN:14701626
CODEN:RCUKB
CAS:alcohol, 64-17-5; progesterone, 57-83-0; tyrosine, 16870-43-2, 55520-40-6, 60-18-4; Anti-Infective Agents, Local; Ethanol
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v155_n6_p529_Sanchez

Referencias:

  • Abel, E.L., Moore, C., Effects of paternal alcohol consumption in mice (1987) Alcoholism Clinical and Experimental Research, 11, pp. 533-535. , https://doi.org/10.1111/j.1530-0277.1987.tb00168.x
  • Jr, A.R.A., Willis, B.R., Oswald, C., Zaneveld, L.J., Ethanol-induced Male infertility: Impairment of spermatozoa (1983) Journal of Pharmacology and Experimental Therapeutics, 225, pp. 479-486
  • Anderson, R.A., Berryman, S.H., Phillips, J.F., Feathergill, K.A., Zaneveld, L.J.D., Russel, L.D., Biochemical and structural evidence for ethanol-induced impairment of testicular development: Apparent lack of Leydig cell involvement (1989) Toxicology and Applied Pharmacology, 100, pp. 62-85. , https://doi.org/10.1016/0041-008X(89)90092-6
  • Anifandis, G., Bounartzi, T., Messini, C.I., Dafopoulos, K., Sotiriou, S., Messinis, I.E., The impact of cigarette smoking and alcohol consumption on sperm parameters and sperm DNA fragmentation (SDF) measured by Halosperm(®) (2014) Archives of Gynecology and Obstetrics, 290, pp. 777-782. , https://doi.org/10.1007/s00404-014-3281-x
  • Auger, J., Eustache, F., Andersen, A.G., Irvine, D.S., Jørgensen, N., Skakkebaek, N.E., Suominen, J., Jouannet, P., Sperm morphological defects related to environment, lifestyle and medical history of 1001 Male partners of pregnant women from four European cities (2001) Human Reproduction, 16, pp. 2710-2717. , https://doi.org/10.1093/humrep/16.12.2710
  • Bailey, J.L., Factors regulating sperm capacitation (2010) System in Biology Reproductive and Medicine, 56, pp. 334-348. , https://doi.org/10.3109/19396368.2010.512377
  • Bonthius, D.J., Tzouras, G., Karacay, B., Mahoney, J., Hutton, A., McKim, R., Pantazis, N.J., Deficiency of neuronal nitric oxide synthase (nNOS) worsens alcohol-induced microencephaly and neuronal loss in developing mice (2002) Developmental Brain Research, 138, pp. 45-59. , https://doi.org/10.1016/S0165-3806(02)00458-3
  • Canteros, M.G., Rettori, V., Franchi, A., Genaro, A., Cebral, E., Faletti, A., Gimeno, M., McCann, S.M., Ethanol inhibits luteinizing hormone-releasing hormone (LHRH) secretion by blocking the response of LHRH neuronal terminals to nitric oxide (1995) PNAS, 92, pp. 3416-3420. , https://doi.org/10.1073/pnas.92.8.3416
  • Cebral, E., Lasserre, A., Rettori, V., De Gimeno, M.A., Impaired mouse fertilization by low chronic alcohol treatment (1997) Alcohol and Alcoholism, 32, pp. 563-572
  • Cebral, E., Lasserre, A., Faletti, A., Gimeno, M.A., Response to ovulatory induction following moderate chronic ethanol administration in mice (1998) Medical Science Research, 26, pp. 29-31
  • Cebral, E., Lasserre, A., Motta, A., Gimeno, M.A.F., Mouse oocyte quality and prostaglandin synthesis by cumulus oocyte complex after moderate chronic ethanol intake (1998) Prostaglandins Leukotrienes and Essential Fatty Acids, 58, pp. 381-387. , https://doi.org/10.1016/S0952-3278(98)90075-8
  • Cebral, E., Abrevaya, X.C., Mudry, M.D., Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice (2011) Cell Biology and Toxicology, 27, pp. 237-248. , https://doi.org/10.1007/s10565-011-9185-7
  • Cicero, T., Badger, T., Effects of alcohol on the hypothalamic-pituitary-gonadal axis in the Male rat (1977) Journal of Pharmacology and Experimental Therapeutics, 201, pp. 427-433
  • Coll, T.A., Chaufan, G., Pérez-Tito, L.G., Ventureira, M.R., Ríos de Molina, M.D.C., Cebral, E., Cellular and molecular oxidative stress-related effects in uterine myometrial and trophoblast-decidual tissues after perigestational alcohol intake up to early mouse organogenesis (2018) Molecular and Cellular Biochemistry, 440, pp. 89-104. , https://doi.org/10.1007/s11010-017-3158-y
  • Condorelli, R.A., Calogero, A.E., Vicari, E., La Vignera, S., Chronic consumption of alcohol and sperm parameters: Our experience and the main evidences (2015) Andrologia, 47, pp. 368-379. , https://doi.org/10.1111/and.12284
  • Creasy, D.M., Chapin, R.E., Testicular and epididymal toxicity: Pathogenesis and potential mechanisms of toxicity (2014) Spermatogenesis, 4, p. e1005511. , https://doi.org/10.1080/21565562.2014.1005511
  • Christova, Y., James, P.S., Jones, R., Lipid diffusion in sperm plasma membranes exposed to peroxidative injury from oxygen free radicals (2004) Molecular Reproduction and Development, 68, pp. 365-372. , https://doi.org/10.1002/mrd.20084
  • Dees, W.L., Skelley, C.W., Hiney, J.K., Johnston, C.A., Actions of ethanol on hypothalamic and pituitary hormones in prepubertal female rats (1990) Alcohol, 7, pp. 21-25. , https://doi.org/10.1016/0741-8329(90)90055-H
  • De Grava Kempinas, W., Klinefelter, G.R., Interpreting histopathology in the epididymis (2014) Spermatogenesis, 4, p. e979114. , https://doi.org/10.4161/21565562.2014.979114
  • De Jong, A.M., Menkveld, R., Lens, J.W., Nienhuis, S.E., Rhemrev, J.P., Effect of alcohol intake and cigarette smoking on sperm parameters and pregnancy (2014) Andrologia, 46, pp. 112-117. , https://doi.org/10.1111/and.12054
  • Eid, N.A., Shibata, M.A., Ito, Y., Kusakabe, K., Hammad, H., Otsuki, Y., Involvement of Fas system and active caspases in apoptotic signalling in testicular germ cells of ethanol-treated rats (2002) International Journal of Andrology, 25, pp. 159-167. , https://doi.org/10.1046/j.13652605.2002.00341.x
  • Evans, J.P., Sperm-egg interaction (2012) Annual Review of Physiology, 74
  • , pp. 477-502. , https://doi.org/10.1146/annurev-physiol-020911-153339; Firns, S., Cruzat, V.F., Keane, K.N., Joesbury, K.A., Lee, A.H., Newsholme, P., Yovich, J.L., The effect of cigarette smoking, alcohol consumption and fruit and vegetable consumption on IVF outcomes: A review and presentation of original data (2015) Reproductive Biology and Endocrinology, 13, p. 134. , https://doi.org/10.1186/s12958-015-0133-x
  • Florman, H.M., Ducibella, T., (2006) Fertilization in Mammals, , 3rd ed. New York, NY: Elsevier Academic Press
  • Fujii, J., Imai, H., Redox reactions in mammalian spermatogenesis and the potential targets of reactive oxygen species under oxidative stress (2014) Spermatogenesis, 4, p. e979108. , https://doi.org/10.4161/21565562.2014.979108
  • Fraser, L.R., Drury, L.M., The relationship between sperm concentration and fertilization in vitro of mouse eggs (1975) Biology of Reproduction, 13, pp. 513-518. , https://doi.org/10.1095/biolreprod13.5.513
  • Gaur, D., Talekar, M., Pathak, V.P., Alcohol intake and cigarette smoking: Impact of two major lifestyle factors on Male fertility (2010) Indian Journal of Pathology and Microbiology, 53, pp. 35-40. , https://doi.org/10.4103/03774929.59180
  • Hansen, M.L., Thulstrup, A.M., Bonde, J.P., Olsen, J., Håkonsen, L.B., Ramlau-Hansen, C.H., Does last week’s alcohol intake affect semen quality or reproductive hormones? A cross-sectional study among healthy young Danish men (2012) Reproductive Toxicology, 34, pp. 457-462. , https://doi.org/10.1016/j.reprotox.2012.06.004
  • Herrero, M.B., Cebral, E., Franchi, A., Motta, A., Gimeno, M.F., Progesterone enhances prostaglandin E2 production via interaction with nitric oxide in the mouse acrosome reaction (1998) Biochemical and Biophysical Research Communications, 252, pp. 324-328. , https://doi.org/10.1006/bbrc.1998.9638
  • Jabbari, S., Sadeghi, M.R., Akhondi, M.M., Ebrahimi Habibi, A., Amirjanati, N., Lakpour, N., Asgharpour, L., Ardekani, A.M., Tyrosine phosphorylation pattern in sperm proteins isolated from normospermic and teratospermic men (2009) Journal of Reproduction and Infertility, 10, pp. 185-191
  • Jensen, T.K., Gottschau, M., Madsen, J.O., Andersson, A.M., Lassen, T.H., Skakkebæk, N.E., Swan, S.H., Jørgensen, N., Habitual alcohol consumption associated with reduced semen quality and changes in reproductive hormones; a cross-sectional study among 1221 young Danish men (2014) BMJ Open, 4, p. e005462. , https://doi.org/10.1136/bmjopen-2014-005462
  • Joo, K.J., Kwon, Y.W., Myung, S.C., Kim, T.H., The effects of smoking and alcohol intake on sperm quality: Light and transmission electron microscopy findings (2012) Journal of International Medical Research, 40, pp. 2327-2335. , https://doi.org/10.1177/030006051204000631
  • Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685. , https://doi.org/10.1038/227680a0
  • Lee, H.Y., Naseer, M.I., Lee, S.Y., Kim, M.O., Time-dependent effect of ethanol on GnRH and GnRH receptor mRNA expression in hypothalamus and testis of adult and pubertal rats (2010) Neuroscience Letters, 471, pp. 25-29. , https://doi.org/10.1016/j.neulet.2010.01.002
  • Lewis-Jones, I., Aziz, N., Seshadri, S., Douglas, A., Howard, P., Sperm chromosomal abnormalities are linked to sperm morphologic deformities (2003) Fertility and Sterilility, 79, pp. 212-215. , https://doi.org/10.1016/S0015-0282(02)04411-4
  • Martini, A.C., Molina, R.I., Estofán, D., Senestrari, D., Fiol de Cuneo, M., Ruiz, R.D., Effects of alcohol and cigarette consumption on human seminal quality (2004) Fertility and Sterility, 82, pp. 374-377. , https://doi.org/10.1016/j.fertnstert.2004.03.022
  • Mittleman, G., Van Brunt, C.L., Matthews, D.B., Schedule-induced ethanol self-administration in DBA/2J and C57BL/6J mice (2003) Alcoholism Clinical and Experimental Research, 97, pp. 918-925. , https://doi.org/10.1111/j.1530-0277.2003.tb04416.x
  • Morton, R.A., Diaz, M.R., Topper, L.A., Valenzuela, C.F., Construction of vapor chambers used to expose mice to alcohol during the equivalent of all three trimesters of human development (2014) Journal of Visualized Experiments, pp. 131-139. , https://doi.org/10.3791/51839
  • Muthusami, K., Chinnaswamy, P., Effect of chronic alcoholism on Male fertility hormones and semen quality (2005) Fertility and Sterility, 84, pp. 919-924. , https://doi.org/10.1016/j.fertnstert.2005.04.025
  • Nicolau, P., Miralpeix, E., Solà, I., Carreras, R., Checa, M.A., Alcohol consumption and in vitro fertilization: A review of the literature (2014) Gynecological Endocrinology, 30, pp. 759-763. , https://doi.org/10.3109/09513590.2014.938623
  • Ogilvie, K., Lee, S., Rivier, C., Effect of three different modes of alcohol administration on the activity of the rat hypothalamic-pituitary-adrenal axis (1997) Alcoholism Clinical and Experimental Research, 21, pp. 467-476. , https://doi.org/10.1111/j.1530-0277.1997.tb03792.x
  • Pajarinen, J., Karhunen, P.J., Savolainen, V., Lalu, K., Penttilä, A., Laippala, P., ; (1996) Moderate Alcohol Consumption and Disorders of Human
  • Spermatogenesis Alcoholism Clinical and Experimental Research, 20
  • , pp. 332-337. , https://doi.org/10.1111/j.1530-0277.1996.tb01648.x; Rahimipour, M., Talebi, A.R., Anvari, M., Sarcheshmeh, A.A., Omidi, M., Effects of different doses of ethanol on sperm parameters, chromatin structure and apoptosis in adult mice (2013) European Journal of Obstetrics and Gynecology and Reproductive Biology, 170, pp. 423-428. , https://doi.org/10.1016/j.ejogrb.2013.06.038
  • Rhodes, J.S., Best, K., Belknap, J.K., Finn, D.A., Crabbe, J.C., Evaluation of a simple model of ethanol drinking to intoxication in C57BL/6J mice (2005) Physiology and Behavior, 84, pp. 53-63. , https://doi.org/10.1016/j.physbeh.2004.10.007
  • Rossi, B.V., Berry, K.F., Hornstein, M.D., Cramer, D.W., Ehrlich, S., Missmer, S.A., Effect of alcohol consumption on in vitro fertilization (2011) Obstetrics and Gynecology, 117, pp. 136-142. , https://doi.org/10.1097/AOG.0b013e31820090e1
  • Salonen, I., Pakarinen, P., Huhtaniemi, I., Effect of chronic ethanol diet on expression of gonadotropin genes in the Male rat (1992) Journal of Pharmacology and Experimental Therapeutics, 260, pp. 463-467
  • Sanchez, M.C., Sedo, C.A., Julianelli, V.L., Romanato, M., Calvo, L., Calvo, J.C., Fontana, V.A., Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation (2013) Systems Biology in Reproductive Medicine, 59, pp. 82-90. , https://doi.org/10.3109/19396368.2012.756952
  • Shayakhmetova, G.M., Bondarenko, L.B., Matvienko, A.V., Kovalenko, V.M., Chronic alcoholism-mediated metabolic disorders in albino rat testes (2014) Interdisciplinary Toxicology, 7, pp. 165-172. , https://doi.org/10.2478/intox-2014-0023
  • Shayakhmetova, G.M., Bondarenko, L.B., Kovalenko, V.M., Ruschak, V.V., CYP2E1 testis expression and alcoholmediated changes of rat spermatogenesis indices and type I collagen (2013) Archives of Industrial Hygiene and Toxicology, 64, pp. 51-60. , https://doi.org/10.2478/10004-1254-64-2013-2313
  • Shirai, T., Ikemoto, I., Mechanism of alcoholic testicular damage (1992) Nippon Hinyokika Gakkai Zasshi, 83, pp. 305-314
  • Sliwowska, J.H., Comeau, W.L., Bodnar, T.S., Ellis, L., Weinberg, J., Prenatal alcohol exposure and pair feeding differentially impact puberty and reproductive development in female rats: Role of the kisspeptin system (2016) Alcoholism: Clinical and Experimental Research, 40, pp. 2368-2376. , https://doi.org/10.1111/acer.13233
  • Stutz, G., Zamudio, J., Santillán, M.E., Vincenti, L., De Cuneo, M.F., Ruiz, R.D., The effect of alcohol, tobacco, and aspirin consumption on seminal quality among healthy young men (2004) Archives of Environmental Health, 59, pp. 548-552. , https://doi.org/10.1080/00039890409603432
  • Talebi, A.R., Sarcheshmeh, A.A., Khalili, M.A., Tabibnejad, N., Effects of ethanol consumption on chromatin condensation and DNA integrity of epididymal spermatozoa in rat (2011) Alcohol, 45, pp. 403-409. , https://doi.org/10.1016/j.alcohol.2010.10.005
  • Towbin, H., Staehelin, T., Gordon, J., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications (1979) PNAS, 76, pp. 4350-4354. , https://doi.org/10.1073/pnas.76.9.4350
  • Välimäki, M., Tuominen, J.A., Huhtaniemi, I., Ylikahri, R., The pulsatile secretion of gonadotropins and growth hormone, and the biological activity of luteinizing hormone in men acutely intoxicated with ethanol (1990) Alcoholism: Clinical and Experimental Research, 14, pp. 928-931
  • Van Heertum, K., Rossi, B., Alcohol and fertility: How much is too much? (2017) Fertility Research and Practice, 3, p. 10. , https://doi.org/10.1186/s40738-017-0037-x
  • Van Thiel, D.H., Disorders of the hypothalamic-pituitary-gonadal and thyroidal axes in patients with liver disease (1990) Hepatology. A Textbook of Liver Disease, pp. 513-529. , 2nd ed., D Zakim & TD Boyer. Philadelphia: Saunders
  • Visconti, P.E., Bailey, J.L., Moore, G.D., Pan, D., Olds-Clarke, P., Kopf, G.S., Capacitation of mouse spermatozoa I. Correlation between the capacitation state and protein tyrosine phosphorylation (1995) Development, 121, pp. 1129-1137
  • Visconti, P.E., Krapf, D., de la Vega-Beltrán, J.L., Acevedo, J.J., Darszon, A., Ion channels, phosphorylation and mammalian sperm capacitation (2011) Asian Journal of Andrology, 13, pp. 395-405. , https://doi.org/10.1038/aja.2010.69
  • Wdowiak, A., Sulima, M., Sadowska, M., Grzegorz, B., Bojar, I., Alcohol consumption and quality of embryos obtained in programmes of in vitro fertilization (2014) Annals of Agricultural and Environmental Medicine, 21, pp. 450-453. , https://doi.org/10.5604/1232-1966.1108623
  • Wieczorek, L., Fish, E.W., O’Leary-Moore, S.K., Parnell, S.E., Sulik, K.K., Hypothalamic-pituitary-adrenal axis and behavioral dysfunction following early binge-like prenatal alcohol exposure in mice (2015) Alcohol, 49, pp. 207-217. , https://doi.org/10.1016/j.alcohol.2015.01.005
  • Willis, B.R., Anderson, R.A., Oswald, C., Zaneveld, L.J., Ethanol-induced Male reproductive tract pathology as a function of ethanol dose and (1983); Duration of exposure Journal Pharmacology Experimental Research, 225
  • Zhang, C.R., Chong, S., Gene expression in the mouse brain following early pregnancy exposure to ethanol (2016) Genomics Data, 10, pp. 107-108. , https://doi.org/10.1016/j.gdata.2016.10.007
  • Zhang, X., Sliwowska, J.H., Weinberg, J., Prenatal alcohol exposure and fetal programming: Effects on neuroendocrine and immune function (2005) Experimental Biology and Medicine, 230, pp. 376-388. , https://doi.org/10.1177/15353702-0323006-05

Citas:

---------- APA ----------
Sánchez, M.C., Fontana, V.A., Galotto, C., Cambiasso, M.Y., Sobarzo, C.M.A., Calvo, L., Calvo, J.C.,..., Cebral, E. (2018) . Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake. Reproduction, 155(6), 529-541.
http://dx.doi.org/10.1530/REP-17-0507
---------- CHICAGO ----------
Sánchez, M.C., Fontana, V.A., Galotto, C., Cambiasso, M.Y., Sobarzo, C.M.A., Calvo, L., et al. "Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake" . Reproduction 155, no. 6 (2018) : 529-541.
http://dx.doi.org/10.1530/REP-17-0507
---------- MLA ----------
Sánchez, M.C., Fontana, V.A., Galotto, C., Cambiasso, M.Y., Sobarzo, C.M.A., Calvo, L., et al. "Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake" . Reproduction, vol. 155, no. 6, 2018, pp. 529-541.
http://dx.doi.org/10.1530/REP-17-0507
---------- VANCOUVER ----------
Sánchez, M.C., Fontana, V.A., Galotto, C., Cambiasso, M.Y., Sobarzo, C.M.A., Calvo, L., et al. Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake. Reproduction. 2018;155(6):529-541.
http://dx.doi.org/10.1530/REP-17-0507