Abstract:
Male chronic alcohol abuse causes testicular failure and infertility. We analyzed the effects of moderate sub-chronic alcohol intake on sperm morphology, capacitation, fertilization and sperm head decondensation. CF-1 male mice were administered 15% ethanol in drinking water for 15 days; control mice received ethanol-free water. Similar patterns of tyrosine phosphorylation were observed in capacitated spermatozoa of control and treated males. Percentage of hyperactivation (H) and spontaneous (SAR) and progesterone-induced (IAR) acrosome reaction significantly decreased at 120 and 150min of capacitation in treated males compared to controls (H: 14.1±2.5 vs 23.7±2.6, P<0.05; SAR-T120min: 17.9±2.5 vs 32.9±4.1, P<0.01; IAR-150min: 43.3±3.5 vs 73.1±1.1, P<0.001, n=6). During in vitro fertilization (2.5, 3.5 and 4.5h post-insemination), there was an increased percentage of fertilized oocytes (with a decondensed sperm head and one or two pronuclei) in treated males (P<0.001, n=7). After 60min of in vitro decondensation with glutathione plus heparin, the percentage of decondensed sperm heads was significantly higher in treated males than in controls (mean±s.d.: 57.1±5.6 vs 48.3±4.5, P<0.05, n=5). The percentage of morphologically normal sperm heads was significantly decreased in treated males with respect to controls (P<0.001, n=9). These results show that short-term moderate alcohol consumption in outbred mice affect sperm morphology, hyperactivation, acrosomal exocytosis, and the dynamics of in vitro fertilization and in vitro sperm nuclear decondensation. © 2018 Society for Reproduction and Fertility.
Registro:
Documento: |
Artículo
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Título: | Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake |
Autor: | Sánchez, M.C.; Fontana, V.A.; Galotto, C.; Cambiasso, M.Y.; Sobarzo, C.M.A.; Calvo, L.; Calvo, J.C.; Cebral, E. |
Filiación: | CONICET, Instituto de Biología y Medicina Experimental (IByME), Buenos Aires, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina Universidad de Buenos Aires, Facultad de Medicina, CONICET-Universidad de Buenos Aires, Instituto de Investigaciones Biomédicas (INBIOMED), Buenos Aires, Argentina Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina CONICET, Universidad de Buenos Aires, Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA-CONICET), Buenos Aires, Argentina
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Palabras clave: | alcohol; progesterone; tyrosine; alcohol; topical antiinfective agent; acrosome reaction; alcohol consumption; animal cell; animal experiment; Article; controlled study; exocytosis; in vitro fertilization; in vitro study; male; mouse; nonhuman; oocyte; oocyte penetration; priority journal; protein phosphorylation; reproduction; spermatozoon capacitation; spermatozoon head; spermatozoon motility; animal; drug effect; fertility; pathology; spermatozoon capacitation; Acrosome Reaction; Animals; Anti-Infective Agents, Local; Ethanol; Fertilization in Vitro; Male; Mice; Oocytes; Sperm Capacitation; Sperm-Ovum Interactions |
Año: | 2018
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Volumen: | 155
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Número: | 6
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Página de inicio: | 529
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Página de fin: | 541
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DOI: |
http://dx.doi.org/10.1530/REP-17-0507 |
Título revista: | Reproduction
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Título revista abreviado: | Reproduction
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ISSN: | 14701626
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CODEN: | RCUKB
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CAS: | alcohol, 64-17-5; progesterone, 57-83-0; tyrosine, 16870-43-2, 55520-40-6, 60-18-4; Anti-Infective Agents, Local; Ethanol
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v155_n6_p529_Sanchez |
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Citas:
---------- APA ----------
Sánchez, M.C., Fontana, V.A., Galotto, C., Cambiasso, M.Y., Sobarzo, C.M.A., Calvo, L., Calvo, J.C.,..., Cebral, E.
(2018)
. Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake. Reproduction, 155(6), 529-541.
http://dx.doi.org/10.1530/REP-17-0507---------- CHICAGO ----------
Sánchez, M.C., Fontana, V.A., Galotto, C., Cambiasso, M.Y., Sobarzo, C.M.A., Calvo, L., et al.
"Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake"
. Reproduction 155, no. 6
(2018) : 529-541.
http://dx.doi.org/10.1530/REP-17-0507---------- MLA ----------
Sánchez, M.C., Fontana, V.A., Galotto, C., Cambiasso, M.Y., Sobarzo, C.M.A., Calvo, L., et al.
"Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake"
. Reproduction, vol. 155, no. 6, 2018, pp. 529-541.
http://dx.doi.org/10.1530/REP-17-0507---------- VANCOUVER ----------
Sánchez, M.C., Fontana, V.A., Galotto, C., Cambiasso, M.Y., Sobarzo, C.M.A., Calvo, L., et al. Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake. Reproduction. 2018;155(6):529-541.
http://dx.doi.org/10.1530/REP-17-0507