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Abstract:

Leptin exerts both stimulatory and inhibitory effects on the ovulatory process. In this study, we investigated whether these opposite effects involve changes in the oxidative status in response to different levels of leptin. To this end, we performed both in vivo and in vitro assays using ovaries of immature rats primed with gonadotropins to induce ovulation. Superoxide dismutase (SOD) and catalase (CAT) activity, lipid peroxidation, glutathione (GSH) content, and reactive oxygen species (ROS) were studied as oxidative damage-related parameters. The expression of BCL2, BAX, and caspase 3 were measured by western blot as apoptosis-related biomarkers. The acute treatment with leptin, which inhibits ovulation, decreased SOD activity and increased active caspase 3 expression. No differences were found in CATactivity, lipid peroxidation, or total GSH. In contrast, the daily administration of leptin, which induces ovulation, decreased GSH content, ROS levels, and Bax and active caspase 3 expression, but caused no changes in other parameters. In addition, the daily administration of leptin induced follicular growth, measured by the number of antral follicles in ovarian sections. Using ovarian explant cultures, we found increased BCL2 expression and decreased SOD activity at low and high concentrations of leptin respectively. Thus, leptin can modulate the oxidative status of the ovarian tissue, during the ovulatory process, by acting on different targets according to its circulating levels. At low concentration, leptin seems to play a protective role against the oxidative stress, whereas at high concentrations, this protein seems to be involved in cell death. © 2015 Society for Reproduction and Fertility.

Registro:

Documento: Artículo
Título:Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats
Autor:Bilbao, M.G.; Di Yorio, M.P.; Galarza, R.A.; Varone, C.L.; Faletti, A.G.
Filiación:Centrode Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Medicina Paraguay 2155, 16oP, Buenos Aires, Argentina
Institutode Quimica Biologica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:caspase 3; catalase; glutathione; gonadotropin; leptin; protein Bax; protein bcl 2; reactive oxygen metabolite; superoxide dismutase; caspase 3; catalase; glutathione; leptin; superoxide dismutase; animal cell; animal experiment; animal tissue; apoptosis; Article; controlled study; explant; female; immaturity; in vitro study; in vivo study; investigative procedures; lipid peroxidation; nonhuman; ovary function; ovulation; oxidative stress; priority journal; protein expression; rat; Western blotting; animal; drug effects; metabolism; ovary; ovulation; oxidation reduction reaction; Sprague Dawley rat; Rattus; Animals; Blotting, Western; Caspase 3; Catalase; Female; Glutathione; Leptin; Lipid Peroxidation; Ovary; Ovulation; Oxidation-Reduction; Oxidative Stress; Rats; Rats, Sprague-Dawley; Superoxide Dismutase
Año:2015
Volumen:149
Número:4
Página de inicio:357
Página de fin:366
DOI: http://dx.doi.org/10.1530/REP-14-0536
Título revista:Reproduction
Título revista abreviado:Reproduction
ISSN:14701626
CODEN:RCUKB
CAS:caspase 3, 169592-56-7; catalase, 9001-05-2; glutathione, 70-18-8; gonadotropin, 63231-54-9; protein bcl 2, 219306-68-0; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Caspase 3; Catalase; Glutathione; Leptin; Superoxide Dismutase
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v149_n4_p357_Bilbao

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Citas:

---------- APA ----------
Bilbao, M.G., Di Yorio, M.P., Galarza, R.A., Varone, C.L. & Faletti, A.G. (2015) . Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats. Reproduction, 149(4), 357-366.
http://dx.doi.org/10.1530/REP-14-0536
---------- CHICAGO ----------
Bilbao, M.G., Di Yorio, M.P., Galarza, R.A., Varone, C.L., Faletti, A.G. "Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats" . Reproduction 149, no. 4 (2015) : 357-366.
http://dx.doi.org/10.1530/REP-14-0536
---------- MLA ----------
Bilbao, M.G., Di Yorio, M.P., Galarza, R.A., Varone, C.L., Faletti, A.G. "Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats" . Reproduction, vol. 149, no. 4, 2015, pp. 357-366.
http://dx.doi.org/10.1530/REP-14-0536
---------- VANCOUVER ----------
Bilbao, M.G., Di Yorio, M.P., Galarza, R.A., Varone, C.L., Faletti, A.G. Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats. Reproduction. 2015;149(4):357-366.
http://dx.doi.org/10.1530/REP-14-0536