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Abstract:

Among several factors known to modulate embryo implantation and survival, uterine quiescence and neovascularization, maternal immunotolerance through the Th1/Th2 cytokine balance towards a Th2 profile, local regulatory T-cell (Treg) activation, and high levels of progesterone were assigned a prominent role. Vasoactive intestinal peptide (VIP) is a neuroimmunopeptide that has anti-inflammatory effects, promotes Th2 cytokines and CD4 +CD25 +FOXP3 + Treg activation, and stimulates exocrine secretion, smooth muscle relaxation, and vasodilatation favoring uterus quiescence. The goal of the present work was to explore the participation of VIP in the implantation sites of normal and pregnant prediabetic nonobese diabetic (NOD) females, a mouse strain that spontaneously develops an autoimmune exocrinopathy similar to Sjögren's syndrome. Our results indicate a reduction in litter size from the third parturition onwards in the NOD female lifespan with increased resorption rates. Progesterone systemic levels were significantly decreased in pregnant NOD mice compared with BALB/c mice, although the allogeneic response to progesterone by spleen cells was not impaired. VIP receptors, Vipr1 and Vipr2 (Vpac1 and Vpac2), were expressed at the implantation sites and VIP induced leukemia inhibitory factor (LIF) and Treg marker expression in both strains; however, a reduced Vip expression was found in NOD implantation sites. We conclude that the reduced birth rate at 16-week-old NOD mice with a Th1 systemic cytokine profile involves resorption processes with a lower expression of VIP at the sites of implantation, which acts as a local inducer of pro-implantatory LIF and Treg activation. © 2009 Society for Reproduction and Fertility.

Registro:

Documento: Artículo
Título:Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice
Autor:Roca, V.; Calafat, M.; Larocca, L.; Ramhorst, R.; Farina, M.; Franchi, A.M.; Pérez Leirós, C.
Filiación:Department of Biological Chemistry, University of Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Laboratory of Physiopathology of Pregnancy and Labor, School of Medicine, University of Buenos Aires, Paraguay 2155, 1121 Buenos Aires, Argentina
Palabras clave:progesterone; vasoactive intestinal polypeptide; vasoactive intestinal polypeptide receptor 1; vasoactive intestinal polypeptide receptor 2; alloimmunity; animal experiment; animal model; animal tissue; article; birth; cell activation; controlled study; embryo resorption; female; immunomodulation; implantation; lifespan; litter size; male; mouse; nonhuman; nonobese diabetic mouse; priority journal; progesterone blood level; protein expression; protein function; regulatory T lymphocyte; Sjoegren syndrome; spleen cell; Th1 cell; Animals; Diabetes, Gestational; Embryo Implantation; Embryo Loss; Female; Immunologic Factors; Litter Size; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred NOD; Prediabetic State; Pregnancy; Receptors, Vasoactive Intestinal Peptide, Type II; Receptors, Vasoactive Intestinal Polypeptide, Type I; T-Lymphocytes, Regulatory; Vasoactive Intestinal Peptide; Mus
Año:2009
Volumen:138
Número:4
Página de inicio:733
Página de fin:742
DOI: http://dx.doi.org/10.1530/REP-09-0171
Título revista:Reproduction
Título revista abreviado:Reproduction
ISSN:14701626
CODEN:RCUKB
CAS:progesterone, 57-83-0; vasoactive intestinal polypeptide, 37221-79-7; Immunologic Factors; Receptors, Vasoactive Intestinal Peptide, Type II; Receptors, Vasoactive Intestinal Polypeptide, Type I; Vasoactive Intestinal Peptide, 37221-79-7; Vipr1 protein, mouse; Vipr2 protein, mouse
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v138_n4_p733_Roca

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Citas:

---------- APA ----------
Roca, V., Calafat, M., Larocca, L., Ramhorst, R., Farina, M., Franchi, A.M. & Pérez Leirós, C. (2009) . Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice. Reproduction, 138(4), 733-742.
http://dx.doi.org/10.1530/REP-09-0171
---------- CHICAGO ----------
Roca, V., Calafat, M., Larocca, L., Ramhorst, R., Farina, M., Franchi, A.M., et al. "Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice" . Reproduction 138, no. 4 (2009) : 733-742.
http://dx.doi.org/10.1530/REP-09-0171
---------- MLA ----------
Roca, V., Calafat, M., Larocca, L., Ramhorst, R., Farina, M., Franchi, A.M., et al. "Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice" . Reproduction, vol. 138, no. 4, 2009, pp. 733-742.
http://dx.doi.org/10.1530/REP-09-0171
---------- VANCOUVER ----------
Roca, V., Calafat, M., Larocca, L., Ramhorst, R., Farina, M., Franchi, A.M., et al. Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice. Reproduction. 2009;138(4):733-742.
http://dx.doi.org/10.1530/REP-09-0171