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Abstract:

We have previously observed expression of prostaglandin-endoperoxide synthase 2 (PTGS2), the key enzyme in the biosynthesis of prostaglandins (PGs), in reproductively active Syrian hamster Leydig cells, and reported an inhibitory role of PGF 2α on hamster testicular steroidogenesis. In this study, we further investigated PTGS2 expression in hamster Leydig cells during sexual development and photoperiodic gonadal regression. Since PTGS2 is mostly expressed in pubertal and reproductively active adult hamsters with high circulating levels of LH and androgens, we studied the role of these hormones in the regulation/maintenance of testicular PTGS2/PGF 2α. In active hamster Leydig cells, LH/hCG and testosterone induced PTGS2 and PGF 2α production, and their actions were abolished by the antiandrogen bicalutamide (Bi). These results indicate that LH does not exert a direct effect on PG synthesis. Testosterone also stimulated phosphorylation of the mitogen-activated protein kinase isoforms 3/1 (MAPK3/1) within minutes and hours, but the testosterone metabolite dihydrotestosterone had no effect on PTGS2 and MAPK3/1. Because Bi and U0126, an inhibitor of the MAP kinase kinases 1 and 2 (MAP2K1/2), abolished testosterone actions on MAPK3/1 and PTGS2, our studies suggest that testosterone directly induces PTGS2/PGF 2α in hamster Leydig cells via androgen receptors and a non-classical mechanism that involves MAPK3/1 activation. Since PGF 2α inhibits testosterone production, it might imply the existence of a regulatory loop that is setting a brake on steroidogenesis. Thus, the androgen environment might be crucial for the regulation of testicular PG production at least during sexual development and photoperiodic variations in hamsters. © 2009 Society for Reproduction and Fertility.

Registro:

Documento: Artículo
Título:Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
Autor:Matzkin, M.E.; Gonzalez-Calvar, S.I.; Mayerhofer, A.; Calandra, R.S.; Frungieri, M.B.
Filiación:Laboratorio de Esteroides, Instituto de Biología Y Medicina Experimental, Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Vuelta de Obligado 2490, Buenos Aires 1428, Argentina
Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1121, Argentina
Institut für Zellbiologie, Universität München, München D-80802, Germany
Instituto Multidisciplinario de Biología Celular, La Plata 1900, Argentina
Palabras clave:1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene; androgen receptor; antiandrogen; bicalutamide; luteinizing hormone; mitogen activated protein kinase 1; mitogen activated protein kinase 3; prostaglandin endoperoxide synthase 2; prostaglandin F2 alpha; prostaglandin synthase; testosterone; unclassified drug; animal cell; animal experiment; animal tissue; article; controlled study; enzyme activation; hormonal regulation; Leydig cell; male; nonhuman; photoperiodicity; priority journal; prostaglandin synthesis; protein expression; protein induction; protein phosphorylation; protein synthesis; sexual development; spermatogenesis; steroidogenesis; Syrian hamster; Androgen Antagonists; Animals; Cells, Cultured; Chorionic Gonadotropin; Cricetinae; Cyclooxygenase 2; Dinoprost; Enzyme Induction; Leydig Cells; Luteinizing Hormone; Male; Mesocricetus; Mitogen-Activated Protein Kinases; Phosphorylation; Photoperiod; Protein Kinase Inhibitors; Receptors, Androgen; Sexual Development; Signal Transduction; Testosterone; Up-Regulation; Cricetinae; Mesocricetus auratus
Año:2009
Volumen:138
Número:1
Página de inicio:163
Página de fin:175
DOI: http://dx.doi.org/10.1530/REP-09-0023
Título revista:Reproduction
Título revista abreviado:Reproduction
ISSN:14701626
CODEN:RCUKB
CAS:1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene, 109511-58-2; bicalutamide, 90357-06-5; luteinizing hormone, 39341-83-8, 9002-67-9; mitogen activated protein kinase 1, 137632-08-7; mitogen activated protein kinase 3, 137632-07-6; prostaglandin F2 alpha, 551-11-1; prostaglandin synthase, 39391-18-9, 59763-19-8, 9055-65-6; testosterone, 58-22-0; Androgen Antagonists; Chorionic Gonadotropin; Cyclooxygenase 2, 1.14.99.1; Dinoprost, 551-11-1; Luteinizing Hormone, 9002-67-9; Mitogen-Activated Protein Kinases, 2.7.11.24; Protein Kinase Inhibitors; Receptors, Androgen; Testosterone, 58-22-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v138_n1_p163_Matzkin

Referencias:

  • Agoulnik, I.U., Bingman III, W.E., Nakka, M., Li, W., Wang, Q., Liu, X.S., Brown, M., Weigel, N.L., Target gene-specific regulation of androgen receptor activity by p42/p44 mitogen-activated protein kinase (2008) Molecular Endocrinology, 22, pp. 2420-2432
  • Ahsan, S., Lacey, M., Whitehead, S.A., Interactions between interleukin-1 beta, nitric oxide and prostaglandin e 2 in the rat ovary: Effects on steroidogenesis (1997) European Journal of Endocrinology, 137, pp. 293-300
  • Balaji, T., Ramanathan, M., Padmanabhan Menon, V., Localization of cyclooxygenase-2 in mice testis and assessment of its possible role through suppressing its expression using nimesulide: A preferential cyclooxygenase-2 inhibitor (2007) Prostaglandins Leukotrienes and Essential Fatty Acids, 76 (6), pp. 341-348. , DOI 10.1016/j.plefa.2007.04.006, PII S0952327807000555
  • Basini, G., Tamanini, C., Interrelationship between nitric oxide and prostaglandins in bovine granulosa cells (2001) Prostaglandins and Other Lipid Mediators, 66 (3), pp. 179-202. , DOI 10.1016/S0090-6980(01)00156-3, PII S0090698001001563
  • Bimonte-Nelson, H.A., Singleton, R.S., Nelson, M.E., Eckman, C.B., Barber, J., Scott, T.Y., Granholm, A.-C.E., Testosterone, but not nonaromatizable dihydrotestosterone, improves working memory and alters nerve growth factor levels in aged male rats (2003) Experimental Neurology, 181 (2), pp. 301-312. , DOI 10.1016/S0014-4886(03)00061-X
  • Chen, H., Luo, L., Liu, J., Zirkin, B.R., Cyclooxygenases in rat Leydig cells: Effects of luteinizing hormone and aging (2007) Endocrinology, 148 (2), pp. 735-742. , http://endo.endojournals.org/cgi/reprint/148/2/735, DOI 10.1210/en.2006-0925
  • Christensen, A.K., A history of studies on testicular Leydig cells: The first century (1996) The Leydig Cell, pp. 1-30. , Eds AH Payne, MP Hardy & LD Russell. Clearwater, FL: Cache River Press
  • Cinar, B., Mukhopadhyay, N.K., Meng, G., Freeman, M.R., Phosphoinositide 3-kinase-independent non-genomic signals transit from the androgen receptor to Akt1 in membrane raft microdomains (2007) Journal of Biological Chemistry, 282 (40), pp. 29584-29593. , http://www.jbc.org/cgi/reprint/282/40/29584, DOI 10.1074/jbc.M703310200
  • Davis, B.J., Lennard, D.E., Lee, C.A., Tiano, H.F., Morham, S.G., Wetsel, W.C., Langenbach, R., Anovulation in cyclooxygenase-2-deficient mice is restored by prostaglandin e 2 and interleukin-1beta (1999) Endocrinology, 140 (6), pp. 2685-2695. , DOI 10.1210/en.140.6.2685
  • Didolkar, A.K., Gurjar, A., Joshi, U.M., Effect of prostaglandins A-1, E-2 and F-2a on blood plasma levels of testosterone, LH and FSH in male rats (1981) Andrologia, 13 (1), pp. 50-55
  • Dinchuk, J.E., Car, B.D., Focht, R.J., Johnston, J.J., Jaffee, B.D., Covington, M.B., Contel, N.R., Czerniak, P.M., Renal abnormalities and an altered inflammatory response in mice lacking cyclooxygenase II (1995) Nature, 378, pp. 406-409
  • Ellis, L.C., Jorgensen, R.D., Age changes in rat testicular capsular and parenchymal delta 13-reductase and 15-hydroxyprostaglandin dehydrogenase activities (1982) Archives of Andrology, 8, pp. 121-128
  • Estrada, M., Espinosa, A., Muller, M., Jaimovich, E., Testosterone stimulates intracellular calcium release and mitogen-activated protein kinases via a G protein-coupled receptor in skeletal muscle cells (2003) Endocrinology, 144 (8), pp. 3586-3597. , DOI 10.1210/en.2002-0164
  • Fix, C., Jordan, C., Cano, P., Walker, W.H., Testosterone activates mitogen-activated protein kinase and the cAMP response element binding protein transcription factor in Sertoli cells (2004) Proceedings of the National Academy of Sciences of the United States of America, 101 (30), pp. 10919-10924. , DOI 10.1073/pnas.0404278101
  • Franchi, A.M., Gimeno, M.F., Gimeno, A.L., Estradiol-17-beta enhances the formation of (3H)-PGF 2α from (3H)-PGE 2 in the uterus isolated from ovariectomized rats (1985) Prostaglandins, 29, pp. 773-783
  • Frungieri, M.B., Gonzalez-Calvar, S.I., Calandra, R.S., Influence of photoinhibition on GABA and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster (1996) International Journal of Andrology, 19, pp. 171-178
  • Frungieri, M.B., Gonzalez-Calvar, S.I., Calandra, R.S., Polyamine levels in testes and seminal vesicles from adult golden hamsters during gonadal regression-recrudescence (1996) Journal of Andrology, 17, pp. 683-691
  • Frungieri, M.B., Gonzalez-Calvar, S.I., Chandrashekar, V., Rao, J.N., Bartke, A., Calandra, R.S., Testicular gamma-aminobutyric acid and circulating androgens in Syrian and Djungarian hamsters during sexual development (1996) International Journal of Andrology, 19, pp. 164-170
  • Frungieri, M.B., Gonzalez-Calvar, S.I., Rubio, M., Ozu, M., Lustig, L., Calandra, R.S., Serotonin in golden hamster testes: Testicular levels, immunolocalization and role during sexual development and photoperiodic regression-recrudescence transition (1999) Neuroendocrinology, 69 (4), pp. 299-308
  • Frungieri, M.B., Weidinger, S., Meineke, V., Kohn, F.M., Mayerhofer, A., Proliferative action of mast-cell tryptase is mediated by PAR2, COX2, prostaglandins, and PPARg: Possible relevance to human fibrotic disorders (2002) Proceedings of the National Academy of Sciences of the United States of America, 99 (23), pp. 15072-15077. , DOI 10.1073/pnas.232422999
  • Frungieri, M.B., Gonzalez-Calvar, S.I., Parborell, F., Albrecht, M., Mayerhofer, A., Calandra, R.S., Cyclooxygenase-2 and prostaglandin F 2α in Syrian hamster Leydig cells: Inhibitory role on luteinizing hormone/ human chorionic gonadotropin-stimulated testosterone production (2006) Endocrinology, 147, pp. 4476-4485
  • Furr, B.J., Valcaccia, B., Curry, B., Woodburn, J.R., Chesterson, G., Tucker, H., ICI 176,334: A novel non-steroidal, peripherally selective antiandrogen (1987) Journal of Endocrinology, 113, pp. R7-R9
  • Ge, R.-S., Hardy, D.O., Catterall, J.F., Hardy, M.P., Opposing changes in 3α-hydroxysteroid dehydrogenase oxidative and reductive activities in rat Leydig cells during pubertal development (1999) Biology of Reproduction, 60, pp. 855-860
  • Gunnarsson, D., Svensson, M., Selstam, G., Nordberg, G., Pronounced induction of testicular PGF 2alpha and suppression of testosterone by cadmium - Prevention by zinc (2004) Toxicology, 200 (1), pp. 49-58. , DOI 10.1016/j.tox.2004.03.003, PII S0300483X04001763
  • Hase, T., Yoshimura, R., Matsuyama, M., Kawahito, Y., Wada, S., Tsuchida, K., Sano, H., Nakatani, T., Cyclooxygenase-1 and -2 in human testicular tumours (2003) European Journal of Cancer, 39, pp. 2043-2049
  • Horton, R., Dihydrotestosterone is a peripheral paracrine hormone (1992) Journal of Andrology, 13, pp. 23-27
  • Hsiao, P.W., Thin, T.H., Lin, D.L., Chang, C., Differential regulation of testosterone vs. 5alpha-dihydrotestosterone by selective androgen response elements (2000) Molecular and Cellular Biochemistry, 206, pp. 169-175
  • Imperato-McGinley, J., Zhu, Y.-S., Androgens and male physiology the syndrome of 5α-reductase-2 deficiency (2002) Molecular and Cellular Endocrinology, 198 (1-2), pp. 51-59. , DOI 10.1016/S0303-7207(02)00368-4, PII S0303720702003684
  • Imperato-McGinley, J., Guerrero, L., Gautier, T., Peterson, R.E., Steroid 5α-reductase deficiency in man: An inherited form of male pseudohermaphroditism (1974) Science, 186, pp. 1213-1215
  • Imperato-McGinley, J., Peterson, R.E., Gautier, T., Sturla, E., Androgens and the evolution of male-gender identity among male pseudohermaphrodites with 5α-reductase deficiency (1979) New England Journal of Medicine, 300 (22), pp. 1233-1237
  • Kang, H.-Y., Cho, C.-L., Huang, K.-L., Wang, J.-C., Hu, Y.-C., Lin, H.-K., Chang, C., Huang, K.-E., Nongenomic androgen activation of phosphatidylinositol 3-kinase/Akt signaling pathway in MC3T3-E1 osteoblasts (2004) Journal of Bone and Mineral Research, 19 (7), pp. 1181-1190. , DOI 10.1359/JBMR.040306
  • Katori, M., Majima, M., Cyclooxygenase-2 its rich diversity of roles and possible application of its selective inhibitors (2000) Inflammation Research, 49, pp. 367-392
  • Kimball, F.A., Kirton, K.T., Forbes, A.D., Frielink, R.D., Porteus, S.E., Wilks, J.W., Mohberg, N.R., Turner, L.F., Serum FSH, LH and testosterone in the male rhesus following prostaglandin injection (1979) Prostaglandins, 18, pp. 117-126
  • Langenbach, R., Morham, S.G., Tiano, H.F., Loftin, C.D., Ghanayem, B.I., Chulada, P.C., Mahler, J.F., Kluckman, K.D., Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration (1995) Cell, 83, pp. 483-492
  • Langenbach, R., Loftin, C.D., Lee, C., Tiano, H., Cyclooxygenase-deficient mice. A summary of their characteristics and susceptibilities to inflammation and carcinogenesis (1999) Annals of the New York Academy of Sciences, 889, pp. 52-61
  • Levy, H., Deane, H.W., Rubin, B.L., Visualization of steroid 3β-oldehydrogenase activity in tissues of intact and hypophysectomized rats (1959) Endocrinology, 65, pp. 932-943
  • Lim, H., Paria, B.C., Das, S.K., Dinchuk, J.E., Langenbach, R., Trzaskos, J.M., Dey, S.K., Multiple female reproductive failures in cyclooxygenase 2-deficient mice (1997) Cell, 91 (2), pp. 197-208. , DOI 10.1016/S0092-8674(00)80402-X
  • Loukil, L.H., Boudawara, T.S., Ayadi, I., Bahloul, A., Jlidi, R., Ayadi, H., Keskes, L.A., High androgen receptor immunoexpression in human 'Sertoli cell only' testis (2005) Archives de L'Institut Pasteur de Tunis, 82, pp. 47-51
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) Journal of Biological Chemistry, 193, pp. 265-275
  • Meineke, V., Frungieri, M.B., Jessberger, B., Vogt, H.-J., Mayerhofer, A., Human testicular mast cells contain tryptase: Increased mast cell number and altered distribution in the testes of infertile men (2000) Fertility and Sterility, 74 (2), pp. 239-244. , DOI 10.1016/S0015-0282(00)00626-9, PII S0015028200006269
  • Mittler, J.C., Studies on the kinetics of the interaction of 7 alphahydroxytestosterone with the steroid 5 alpha-reductase (1985) Steroids, 45, pp. 135-142
  • Neeraja, S., Sreenath, A.S., Reddy, P.R., Reddanna, P., Expression of cyclooxygenase-2 in rat testis (2003) Reproductive Biomedicine Online, 6, pp. 302-309
  • Nemetallah, B.R., Ellis, L.C., Prostaglandin dehydrogenase activity of rat and rabbit testicular tissues and accessory glands before and after castration (1985) Journal of Andrology, 6, pp. 97-101
  • O'Neill, G.P., Ford-Hutchinson, A.W., Expression of mRNA for cyclooxygenase-1 and cyclooxygenase-2 in human tissues (1993) FEBS Letters, 330 (2), pp. 156-160
  • Pelletier, G., Localization of androgen and estrogen receptors in rat and primate tissues (2000) Histology and Histopathology, 15 (4), pp. 1261-1270
  • Rapid signalling by androgen receptor in prostate cancer cells Oncogene, 18, pp. 6322-6329
  • Pinthus, J.H., Bryskin, I., Trachtenberg, J., Lu, J.-P., Singh, G., Fridman, E., Wilson, B.C., Androgen induces adaptation to oxidative stress in prostate cancer: Implications for treatment with radiation therapy (2007) Neoplasia, 9 (1), pp. 68-80. , http://www.neoplasia.com/pdf/manuscript/neo06739.pdf, DOI 10.1593/neo.06739
  • Rahman, F., Christian, H.C., Non-classical actions of testosterone: An update (2007) Trends in Endocrinology and Metabolism, 18, pp. 371-378
  • Van Roijen, J.H., Van Assen, S., Van Der Kwast, T.H., De Rooij, D.G., Boersma, W.J., Vreeburg, J.T., Weber, R.F., Androgen receptor immunoexpression in the testes of subfertile men (1995) Journal of Andrology, 16, pp. 510-516
  • Romanelli, F., Valenca, M., Conte, D., Isidori, A., Negro-Vilar, A., Arachidonic acid and its metabolites effects on testosterone production by rat Leydig cells (1995) Journal of Endocrinological Investigation, 18, pp. 186-193
  • Rosness, P.A., Sunde, A., Eik Nes, K.B., Production and effects of 7 alpha hydroxytestosterone on testosterone and dihydrotestosterone metabolism in rat testis (1977) Biochimica et Biophysica Acta, 488 (1), pp. 55-68
  • Saksena, S.K., El-Safoury, S., Bartke, A., Prostaglandins e 2 and F 2 decrease plasma testosterone levels in male rats (1973) Prostaglandins, 4, pp. 235-242
  • Sawada, T., Asada, M., Mori, J., Effects of single and repeated administration of prostaglandin F(2a) on secretion of testosterone by male rats (1994) Prostaglandins, 47 (5), pp. 345-352. , DOI 10.1016/0090-6980(94)90052-3
  • Schams, D., Berisha, B., Regulation of corpus luteum function in cattle - An overview (2004) Reproduction in Domestic Animals, 39 (4), pp. 241-251. , DOI 10.1111/j.1439-0531.2004.00509.x
  • Schell, C., Frungieri, M.B., Albrecht, M., Gonzalez-Calvar, S.I., Köhn, F.M., Calandra, R.S., Mayerhofer, A., A prostaglandin D2 system in the human testis (2007) Fertility and Sterility, 88, pp. 233-236
  • Smith, W.L., DeWitt, D.L., Garavito, R.M., Cyclooxygenases: Structural, cellular and molecular biology (2000) Annual Review of Biochemistry, 69, pp. 149-182
  • Sonderfan, A.J., Arloto, M.P., Parkinson, A., Identification of the cytochrome P-450 isozymes responsible for testosterone oxidation in rat lung, kidney, and testis: Evidence that cytochrome P-450a (P450IIA1) is the physiologically important testosterone 7α-hydroxylase in rat testis (1989) Endocrinology, 125 (2), pp. 857-866
  • Syntin, P., Chen, H., Zirkin, B.R., Robaire, B., Gene expression in Brown Norway rat Leydig cells: Effects of age and of age-related germ cell loss (2001) Endocrinology, 142 (12), pp. 5277-5285. , DOI 10.1210/en.142.12.5277
  • Tai, C.-J., Kang, S.K., Choi, K.-C., Tzeng, C.-R., Leung, P.C.K., Role of mitogen-activated protein kinase in prostaglandin F 2alpha action in human granulosa-luteal cells (2001) Journal of Clinical Endocrinology and Metabolism, 86 (1), pp. 375-380. , DOI 10.1210/jc.86.1.375
  • Viger, R.S., Robaire, B., Steady state steroid 5α-reductase messenger ribonucleic acid levels and immunocytochemical localization of the type I protein in the rat testis during postnatal development (1995) Endocrinology, 136, pp. 5409-5415
  • Wang, X., Dyson, M.T., Jo, Y., Stocco, D.M., Inhibition of cyclooxygenase-2 activity enhances steroidogenesis and steroidogenic acute regulatory gene expression in MA-10 mouse Leydig cells (2003) Endocrinology, 144 (8), pp. 3368-3375. , DOI 10.1210/en.2002-0081
  • Wang, X., Shen, C.-L., Dyson, M.T., Eimerl, S., Orly, J., Hutson, J.C., Stocco, D.M., Cyclooxygenase-2 regulation of the age-related decline in testosterone biosynthesis (2005) Endocrinology, 146 (10), pp. 4202-4208. , http://endo.endojournals.org/cgi/reprint/146/10/4202, DOI 10.1210/en.2005-0298
  • Winnall, W.R., Ali, U., O'Bryan, M.K., Hirst, J.J., Whiley, P.A.F., Muir, J.A., Hedger, M.P., Constitutive expression of prostaglandin-endoperoxide synthase 2 by somatic and spermatogenic cells is responsible for prostaglandin e 2 production in the adult rat testis (2007) Biology of Reproduction, 76, pp. 759-768
  • Wohlrab, F., Essbach, H., Histochemical and microgel electrophoretical investigations of prostaglandin dehydrogenating enzymes in rabbit kidney (1984) Histochemical Journal, 16 (4), pp. 412-415
  • Zhou, Q., Nie, R., Prins, G.S., Saunders, P.T.K., Katzenellenbogen, B.S., Hess, R.A., Localization of androgen and estrogen receptors in adult male mouse reproductive tract (2002) Journal of Andrology, 23 (6), pp. 870-881
  • Zhu, X., Li, H., Liu, J.-P., Funder, J.W., Androgen stimulates mitogen-activated protein kinase in human breast cancer cells (1999) Molecular and Cellular Endocrinology, 152 (1-2), pp. 199-206. , DOI 10.1016/S0303-7207(99)00031-3, PII S0303720799000313
  • Zhu, L.-J., Hardy, M.P., Inigo, I.V., Huhtaniemi, I., Bardin, C.W., Moo-Young, A.J., Effects of androgen on androgen receptor expression in rat testicular and epididymal cells: A quantitative immunohistochemical study (2000) Biology of Reproduction, 63 (2), pp. 368-376

Citas:

---------- APA ----------
Matzkin, M.E., Gonzalez-Calvar, S.I., Mayerhofer, A., Calandra, R.S. & Frungieri, M.B. (2009) . Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells. Reproduction, 138(1), 163-175.
http://dx.doi.org/10.1530/REP-09-0023
---------- CHICAGO ----------
Matzkin, M.E., Gonzalez-Calvar, S.I., Mayerhofer, A., Calandra, R.S., Frungieri, M.B. "Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells" . Reproduction 138, no. 1 (2009) : 163-175.
http://dx.doi.org/10.1530/REP-09-0023
---------- MLA ----------
Matzkin, M.E., Gonzalez-Calvar, S.I., Mayerhofer, A., Calandra, R.S., Frungieri, M.B. "Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells" . Reproduction, vol. 138, no. 1, 2009, pp. 163-175.
http://dx.doi.org/10.1530/REP-09-0023
---------- VANCOUVER ----------
Matzkin, M.E., Gonzalez-Calvar, S.I., Mayerhofer, A., Calandra, R.S., Frungieri, M.B. Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells. Reproduction. 2009;138(1):163-175.
http://dx.doi.org/10.1530/REP-09-0023