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Abstract:

A functional interaction between progesterone, Th2 cytokines and a suitable balance between nitric oxide and prostaglandins in the uterus is considered to have a major role in the success of embryo implantation and pregnancy. Non-obese diabetic (NOD) mice offer a suitable model to study the modulatory role of Th1 cytokines on uterus signalling and function, since at the prediabetic stage they develop a spontaneous Th1 autoimmune response against exocrine glands similar to Sjögren's syndrome. Vasoactive intestinal peptide (VIP) is a vasoactive neuro- and immunopeptide that promotes Th2 profiles and contributes to the smooth muscle relaxation and vasodilation. The aim of the present study was to investigate the activities of nitric oxide synthase and cyclo-oxygenase and the effect of VIP in the uterus of NOD mice with an emerging Th1 cytokine response. We present evidence of a reduced basal and VIP-stimulated activity of both enzymes in the uterus of NOD mice compared with normal BALB/c mice in proestrus. An altered functional interaction between both enzymes is also present in NOD mice at the time when increased levels of serum interleukin (IL)-12 and tumour necrosis factor-α but not interferon (IFN)-γ or IL-10 were detected. We conclude that signalling alterations in uteri of NOD mice are simultaneous to the onset of a systemic Th1 cytokine response. © 2006 Society for Reproduction and Fertility.

Registro:

Documento: Artículo
Título:Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice
Autor:Roca, V.; Larocca, L.; Calafat, M.; Aisemberg, J.; Meiss, R.; Franchi, A.M.; Leirós, C.P.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Universidad de Buenos Aires, CONICET, Buenos Aires, Argentina
Academia Nacional de Medicina, Buenos Aires, Argentina
Ciudad Universitaria, Pab. 11, 40 piso, 1428 Buenos Aires, Argentina
Palabras clave:cytokine; gamma interferon; interleukin 10; interleukin 12; neuropeptide; nitric oxide; nitric oxide synthase; progesterone; prostaglandin; prostaglandin synthase; tumor necrosis factor alpha; vasoactive intestinal polypeptide; animal experiment; animal model; animal tissue; article; autoimmunity; controlled study; diabetes mellitus; enzyme activity; exocrine gland; female; impaired glucose tolerance; mouse; nidation; nonhuman; pregnancy; priority journal; proestrus; protein interaction; signal transduction; Sjoegren syndrome; smooth muscle relaxation; Th1 cell; Th2 cell; uterus; vasodilatation; Animals; Cyclooxygenase Inhibitors; Diabetes Mellitus, Type 1; Dinoprostone; Enzyme Activation; Female; Immunohistochemistry; Indomethacin; Interferon Type II; Interleukin-10; Interleukin-12; Mice; Mice, Inbred BALB C; Mice, Inbred NOD; Models, Animal; Nitric Oxide Synthase; omega-N-Methylarginine; Pregnancy; Prostaglandin-Endoperoxide Synthases; Sjogren's Syndrome; Th1 Cells; Tumor Necrosis Factor-alpha; Uterus; Vasoactive Intestinal Peptide
Año:2006
Volumen:132
Número:6
Página de inicio:931
Página de fin:938
DOI: http://dx.doi.org/10.1530/rep.1.01050
Título revista:Reproduction
Título revista abreviado:Reproduction
ISSN:14701626
CODEN:RCUKB
CAS:gamma interferon, 82115-62-6; interleukin 12, 138415-13-1; nitric oxide synthase, 125978-95-2; nitric oxide, 10102-43-9; progesterone, 57-83-0; prostaglandin synthase, 39391-18-9, 59763-19-8, 9055-65-6; vasoactive intestinal polypeptide, 37221-79-7; Cyclooxygenase Inhibitors; Dinoprostone, 363-24-6; Indomethacin, 53-86-1; Interferon Type II, 82115-62-6; Interleukin-10, 130068-27-8; Interleukin-12, 187348-17-0; Nitric Oxide Synthase, EC 1.14.13.39; omega-N-Methylarginine, 17035-90-4; Prostaglandin-Endoperoxide Synthases, EC 1.14.99.1; Tumor Necrosis Factor-alpha; Vasoactive Intestinal Peptide, 37221-79-7
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_14701626_v132_n6_p931_Roca.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v132_n6_p931_Roca

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Citas:

---------- APA ----------
Roca, V., Larocca, L., Calafat, M., Aisemberg, J., Meiss, R., Franchi, A.M. & Leirós, C.P. (2006) . Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice. Reproduction, 132(6), 931-938.
http://dx.doi.org/10.1530/rep.1.01050
---------- CHICAGO ----------
Roca, V., Larocca, L., Calafat, M., Aisemberg, J., Meiss, R., Franchi, A.M., et al. "Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice" . Reproduction 132, no. 6 (2006) : 931-938.
http://dx.doi.org/10.1530/rep.1.01050
---------- MLA ----------
Roca, V., Larocca, L., Calafat, M., Aisemberg, J., Meiss, R., Franchi, A.M., et al. "Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice" . Reproduction, vol. 132, no. 6, 2006, pp. 931-938.
http://dx.doi.org/10.1530/rep.1.01050
---------- VANCOUVER ----------
Roca, V., Larocca, L., Calafat, M., Aisemberg, J., Meiss, R., Franchi, A.M., et al. Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice. Reproduction. 2006;132(6):931-938.
http://dx.doi.org/10.1530/rep.1.01050