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Abstract:

Apoptosis is associated with the regression of the corpus luteum (CL) in many species. Since caspases play a central role in apoptosis, we studied several initiators (-2, -8, and -9) and the main effector (-3) caspase in the CL during the estrous cycle of the rat. Two different populations of CL (old and new) were identified on ovaries at estrus and diestrus II (DII). Diminished (P<0.05) luteal progesterone content and P450scc levels suggested that functional luteolysis occurred between the new CL at DII and old CL at estrus, whereas the decline (P<0.05) in luteal weight indicated that structural regression was occurring between old CL at estrus to DII. Immunostaining for caspase-2 in luteal and endothelial cells appeared to increase as the luteal phase progressed, peaking at DII in the old CL. However, caspase-8 and -9 immunostaining showed little change with a slight increase at estrus in the old population. Notably, caspase-3 staining appeared to peak at DII in the new CL. Enzyme activity of caspase-9 increased (P<0.05) in the new CL at DII, followed by that of caspase-2 and -3 in old CL at estrus. Caspase-8 activity did not change at any stage. The number of apoptotic cells increased at DII in the old CL. These results suggest an important role for this protease family during early events of luteolysis in the rat estrous cycle. © 2006 Society for Reproduction and Fertility.

Registro:

Documento: Artículo
Título:Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle
Autor:Peluffo, M.C.; Bussmann, L.; Stouffer, R.L.; Tesone, M.
Filiación:Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR 97006, United States
Instituto de Biología y Medicina Experimental (IBYME-CONICET), Departamento de Química Biológica, Universidad de Buenos Aires, Vuelta de Obligado 2490, Buenos Aires, Argentina
Palabras clave:caspase 2; caspase 3; caspase 8; caspase 9; cytochrome P450; progesterone; animal tissue; apoptosis; article; controlled study; corpus luteum; diestrus; endothelium cell; enzyme activity; estrus cycle; female; immunohistochemistry; luteal cell; luteal phase; nick end labeling; nonhuman; priority journal; protein analysis; protein content; protein expression; protein function; rat; statistical significance; Western blotting; Animals; Apoptosis; Blotting, Western; Caspase 2; Caspase 3; Caspase 8; Caspase 9; Caspases; Cholesterol Side-Chain Cleavage Enzyme; Corpus Luteum; Estrous Cycle; Female; Immunohistochemistry; In Situ Nick-End Labeling; Progesterone; Rats; Rats, Sprague-Dawley
Año:2006
Volumen:132
Número:3
Página de inicio:465
Página de fin:475
DOI: http://dx.doi.org/10.1530/rep.1.00910
Título revista:Reproduction
Título revista abreviado:Reproduction
ISSN:14701626
CODEN:RCUKB
CAS:caspase 2, 182372-14-1; caspase 3, 169592-56-7; caspase 9, 180189-96-2; cytochrome P450, 9035-51-2; progesterone, 57-83-0; Caspase 2, 3.4.22.-; Caspase 3, 3.4.22.-; Caspase 8, 3.4.22.-; Caspase 9, 3.4.22.-; Caspases, 3.4.22.-; Cholesterol Side-Chain Cleavage Enzyme, 1.14.15.6; Progesterone, 57-83-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v132_n3_p465_Peluffo

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Citas:

---------- APA ----------
Peluffo, M.C., Bussmann, L., Stouffer, R.L. & Tesone, M. (2006) . Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle. Reproduction, 132(3), 465-475.
http://dx.doi.org/10.1530/rep.1.00910
---------- CHICAGO ----------
Peluffo, M.C., Bussmann, L., Stouffer, R.L., Tesone, M. "Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle" . Reproduction 132, no. 3 (2006) : 465-475.
http://dx.doi.org/10.1530/rep.1.00910
---------- MLA ----------
Peluffo, M.C., Bussmann, L., Stouffer, R.L., Tesone, M. "Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle" . Reproduction, vol. 132, no. 3, 2006, pp. 465-475.
http://dx.doi.org/10.1530/rep.1.00910
---------- VANCOUVER ----------
Peluffo, M.C., Bussmann, L., Stouffer, R.L., Tesone, M. Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle. Reproduction. 2006;132(3):465-475.
http://dx.doi.org/10.1530/rep.1.00910