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Abstract:

Bovine sperm protease, 66 kDa (BSp66) is a serine protease previously characterized in bovine spermatozoa. Like other proteases, it may be present in sperm from other mammalian species different from bovine, playing a role in the fertilization process. In this study, we looked for BSp66 in hamster spermatozoa using heterologous antibodies against bovine BSp66. An immunoreactive protein was detected by Western blotting in mature and immature sperm. The detected protein had two isoforms similar to the ones reported in bovine sperm. Furthermore, indirect immune detection by fluorescence and electron microscopy assays, showed BSp66 signal at the acrosomal region similar to bovine sperm. As it was determined in bovine sperm, the acrosomal reaction displays the antigen within the acrosomal content. When live hamster sperm was incubated with polyclonal antibody against bovine BSp66 a decrease in the number of sperm bound to zona pellucida in homologous IVF and an impairment of head-head agglutination, were observed. These results suggest that a protease homologous to bovine BSp66 is present in golden hamster spermatozoa, with a conserved molecular mass and cellular location. Moreover, hamster BSp66 is probably involved in zona pellucida recognition. © 2005 Society for Reproduction and Fertility.

Registro:

Documento: Artículo
Título:Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction
Autor:Cesari, A.; Katunar, M.R.; Monclus, M.A.; Vincenti, A.; de Rosas, J.C.; Fornés, M.W.
Filiación:Instituto de Histologia y Embrologia, Area de Histologia y Embriologia, Universidad Nacional de Cuyo, 5500 Mendoza, Argentina
Inst. de Investigaciones Biologicas, Fac. de Ciencias Exactas y Naturales, Univ. Nacional de Mar del Plata, 7600 Mar del Plata, Buenos Aires, Argentina
Idioma: Inglés
Palabras clave:antigen; isoprotein; polyclonal antibody; serine proteinase; acrosome reaction; animal cell; animal experiment; animal tissue; antigen antibody reaction; article; cell function; controlled study; cow; electron microscopy; enzyme activity; female; fertilization; fertilization in vitro; hamster; immunofluorescence; immunoreactivity; male; molecular weight; nonhuman; nucleotide sequence; priority journal; protein localization; sequence homology; signal transduction; spermatozoon; spermatozoon count; zona pellucida; Animals; Cattle; Cell Culture Techniques; Cell Survival; Cricetinae; Fertilization in Vitro; Immunohistochemistry; Male; Microscopy, Immunoelectron; Serine Endopeptidases; Sperm Motility; Sperm-Ovum Interactions; Spermatozoa; Bovinae; Cricetinae; Mammalia; Mesocricetus auratus
Año:2005
Volumen:129
Número:3
Página de inicio:291
Página de fin:298
DOI: http://dx.doi.org/10.1530/rep.1.00375
Título revista:Reproduction
Título revista abreviado:Reproduction
ISSN:14701626
CODEN:RCUKB
CAS:serine proteinase, 37259-58-8; Serine Endopeptidases, EC 3.4.21.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v129_n3_p291_Cesari

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Citas:

---------- APA ----------
Cesari, A., Katunar, M.R., Monclus, M.A., Vincenti, A., de Rosas, J.C. & Fornés, M.W. (2005) . Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction. Reproduction, 129(3), 291-298.
http://dx.doi.org/10.1530/rep.1.00375
---------- CHICAGO ----------
Cesari, A., Katunar, M.R., Monclus, M.A., Vincenti, A., de Rosas, J.C., Fornés, M.W. "Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction" . Reproduction 129, no. 3 (2005) : 291-298.
http://dx.doi.org/10.1530/rep.1.00375
---------- MLA ----------
Cesari, A., Katunar, M.R., Monclus, M.A., Vincenti, A., de Rosas, J.C., Fornés, M.W. "Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction" . Reproduction, vol. 129, no. 3, 2005, pp. 291-298.
http://dx.doi.org/10.1530/rep.1.00375
---------- VANCOUVER ----------
Cesari, A., Katunar, M.R., Monclus, M.A., Vincenti, A., de Rosas, J.C., Fornés, M.W. Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction. Reproduction. 2005;129(3):291-298.
http://dx.doi.org/10.1530/rep.1.00375