Artículo

Martina, P.; Leguizamon, M.; Prieto, C.I.; Sousa, S.A.; Montanaro, P.; Draghi, W.O.; Stämmler, M.; Bettiol, M.; de Carvalho, C.C.C.R.; Palau, J.; Figoli, C.; Alvarez, F.; Benetti, S.; Lejona, S.; Vescina, C.; Ferreras, J.; Lasch, P.; Lagares, A. (...) Bosch, A. "Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils" (2018) International Journal of Systematic and Evolutionary Microbiology. 68(1):14-20
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Bacteria from the Burkholderia cepacia complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-year epidemiological surveillance involving Bcc bacteria from respiratory secretions of Argentinean patients with CF and from hospital settings, we found six isolates of the Bcc with a concatenated species-specific allele sequence that differed by more than 3% from those of the Bcc with validly published names. According to the multilocus sequence analysis (MLSA), these isolates clustered with the agricultural soil strain, Burkholderia sp. PBP 78, which was already deposited in the PubMLST database. The isolates were examined using a polyphasic approach, which included 16S rRNA, recA, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), DNA base composition, average nucleotide identities (ANIs), fatty acid profiles, and biochemical characterizations. The results of the present study demonstrate that the seven isolates represent a single novel species within the Bcc, for which the name Burkholderia puraquae sp. nov. is proposed. Burkholderia puraquae sp. nov. CAMPA 1040T (=LMG 29660T=DSM 103137T) was designated the type strain of the novel species, which can be differentiated from other species of the Bcc mainly from recA gene sequence analysis, MLSA, ANIb, MALDI-TOF MS analysis, and some biochemical tests, including the ability to grow at 42 °C, aesculin hydrolysis, and lysine decarboxylase and β-galactosidase activities. © 2018 IUMS.

Registro:

Documento: Artículo
Título:Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils
Autor:Martina, P.; Leguizamon, M.; Prieto, C.I.; Sousa, S.A.; Montanaro, P.; Draghi, W.O.; Stämmler, M.; Bettiol, M.; de Carvalho, C.C.C.R.; Palau, J.; Figoli, C.; Alvarez, F.; Benetti, S.; Lejona, S.; Vescina, C.; Ferreras, J.; Lasch, P.; Lagares, A.; Zorreguieta, A.; Leitão, J.H.; Yantorno, O.M.; Bosch, A.
Filiación:CINDEFI, CONICET-CCT La Plata, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina
iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, Lisboa, Portugal
Hospital Santísima Trinidad de Córdoba, Córdoba, Argentina
Fundación Instituto Leloir and IIBA-Consejo Nacional de Investigaciones Científicas y Tecnológicas, Buenos aires, Argentina
IBBM, CONICET-CCT La Plata, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina
Proteomics and Spectroscopy Unit (ZBS6) at the Centre for Biological Threats and Special Pathogens, Robert Koch-Institut, Berlin, Germany
Sala de Microbiología, Hospital de Niños «Sor María Ludovica», La Plata, Buenos Aires, Argentina
Laboratorio Cemar D. S. L. A. C, Municipalidad de Rosario, Santa Fe, Argentina
IBS, CONICET-CCT Nordeste, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Misiones, Argentina
Palabras clave:Burkholderia cepacia complex; Burkholderia genus; Burkholderia puraquae sp. nov; bacterial DNA; fatty acid; RNA 16S; agriculture; bacterial gene; bacterium identification; Burkholderia cepacia complex; chemistry; classification; cystic fibrosis; DNA base composition; DNA sequence; genetics; human; microbiology; multilocus sequence typing; phylogeny; species difference; sputum; Agriculture; Bacterial Typing Techniques; Base Composition; Burkholderia cepacia complex; Cystic Fibrosis; DNA, Bacterial; Fatty Acids; Genes, Bacterial; Humans; Multilocus Sequence Typing; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Species Specificity; Sputum
Año:2018
Volumen:68
Número:1
Página de inicio:14
Página de fin:20
DOI: http://dx.doi.org/10.1099/ijsem.0.002293
Título revista:International Journal of Systematic and Evolutionary Microbiology
Título revista abreviado:Int. J. Syst. Evol. Microbiol.
ISSN:14665026
CODEN:ISEMF
CAS:DNA, Bacterial; Fatty Acids; RNA, Ribosomal, 16S
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14665026_v68_n1_p14_Martina

Referencias:

  • Coenye, T., Vandamme, P., Diversity and significance of Burkholderia species occupying diverse ecological niches (2003) Environ Microbiol, 5, pp. 719-729
  • Vanlaere, E., Baldwin, A., Gevers, D., Henry, D., De Brandt, E., Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. Nov. and Burkholderia lata sp. nov (2009) Int J Syst Evol Microbiol, 59, pp. 102-111
  • Peeters, C., Zlosnik, J.E., Spilker, T., Hird, T.J., Lipuma, J.J., Burkholderia pseudomultivorans sp. Nov., a novel Burkholderia cepacia complex species from human respiratory samples and the rhizosphere (2013) Syst Appl Microbiol, 36, pp. 483-489
  • De Smet, B., Mayo, M., Peeters, C., Zlosnik, J.E., Spilker, T., Burkholderia stagnalis sp. Nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources (2015) Int J Syst Evol Microbiol, 65, pp. 2265-2271
  • Baldwin, A., Mahenthiralingam, E., Drevinek, P., Vandamme, P., Govan, J.R., Environmental Burkholderia cepacia complex isolates in human infections (2007) Emerg Infect Dis, 13, pp. 458-461
  • Martin, M., Christiansen, B., Caspari, G., Hogardt, M., Von Thomsen, A.J., Hospital-wide outbreak of Burkholderia contaminans caused by prefabricated moist washcloths (2011) J Hosp Infect, 77, pp. 267-270
  • Drevinek, P., Mahenthiralingam, E., Burkholderia cenocepacia in cystic fibrosis: Epidemiology and molecular mechanisms of virulence (2010) Clin Microbiol Infect, 16, pp. 821-830
  • Abe, K., D’Angelo, M.T., Sunenshine, R., Noble-Wang, J., Cope, J., Outbreak of Burkholderia cepacia bloodstream infection at an outpatient hematology and oncology practice (2007) Infect Control Hosp Epidemiol, 28, pp. 1311-1313
  • Martina, P., Bettiol, M., Vescina, C., Montanaro, P., Mannino, M.C., Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina (2013) J Clin Microbiol, 51, pp. 339-344
  • Heo, S.T., Kim, S.J., Jeong, Y.G., Bae, I.G., Jin, J.S., Hospital outbreak of Burkholderia stabilis bacteraemia related to contaminated chlorhexidine in haematological malignancy patients with indwelling catheters (2008) J Hosp Infect, 70, pp. 241-245
  • Mahenthiralingam, E., Bischof, J., Byrne, S.K., Radomski, C., Davies, J.E., DNA-Based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III (2000) J Clin Microbiol, 38, pp. 3165-3173
  • Mahenthiralingam, E., Baldwin, A., Vandamme, P., Burkholderia cepacia complex infection in patients with cystic fibrosis (2002) J Med Microbiol, 51, pp. 533-538
  • Papaleo, M.C., Perrin, E., Maida, I., Fondi, M., Fani, R., Identification of species of the Burkholderia cepacia complex by sequence analysis of the hisA gene (2010) J Med Microbiol, 59, pp. 1163-1170
  • Baldwin, A., Mahenthiralingam, E., Thickett, K.M., Honeybourne, D., Maiden, M.C., Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex (2005) J Clin Microbiol, 43, pp. 4665-4673
  • Vanlaere, E., Lipuma, J.J., Baldwin, A., Henry, D., De Brandt, E., Burkholderia latens sp. Nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex (2008) Int J Syst Evol Microbiol, 58, pp. 1580-1590
  • Konstantinidis, K.T., Tiedje, J.M., Genomic insights that advance the species definition for prokaryotes (2005) Proc Natl Acad Sci USA, 102, pp. 2567-2572
  • Richter, M., Rosselló-Móra, R., Shifting the genomic gold standard for the prokaryotic species definition (2009) Proc Natl Acad Sci USA, 106, pp. 19126-19131
  • Martina, P., Feliziani, S., Juan, C., Bettiol, M., Gatti, B., Hypermutation in Burkholderia cepacia complex is mediated by DNA mismatch repair inactivation and is highly prevalent in cystic fibrosis chronic respiratory infection (2014) Int J Med Microbiol, 304, pp. 1182-1191
  • (2005) Standard Methods for the Examination of Water and Wastewater, , 21st ed. Washington, DC: APHA
  • Draghi, W.O., Peeters, C., Cnockaert, M., Snauwaert, C., Wall, L.G., Burkholderia cordobensis sp. Nov., from agricultural soils (2014) Int J Syst Evol Microbiol, 64, pp. 2003-2008
  • Lane, D.J., 16S/23S rRNA sequencing (1991) Nucleic Acid Techniques in Bacterial Systematics, pp. 115-176. , Stackebrandt EGM (editor), New York: John Wiley & Sons, Inc
  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S., MEGA6: Molecular evolutionary genetics analysis version 6.0 (2013) Mol Biol Evol, 30, pp. 2725-2729
  • Desai, A.P., Stanley, T., Atuan, M., McKey, J., Lipuma, J.J., Use of matrix assisted laser desorption ionisation-time of flight mass spectrometry in a paediatric clinical laboratory for identification of bacteria commonly isolated from cystic fibrosis patients (2012) J Clin Pathol, 65, pp. 835-838
  • Miñán, A., Bosch, A., Lasch, P., Stämmler, M., Serra, D.O., Rapid identification of Burkholderia cepacia complex species including strains of the novel Taxon K, recovered from cystic fibrosis patients by intact cell MALDI-ToF mass spectrometry (2009) Analyst, 134, pp. 1138-1148
  • Lambiase, A., Del Pezzo, M., Cerbone, D., Raia, V., Rossano, F., Rapid identification of Burkholderia cepacia complex species recovered from cystic fibrosis patients using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (2013) J Microbiol Methods, 92, pp. 145-149
  • Lasch, P., Naumann, D., MALDI-TOF mass spectrometry for the rapid identification of highly pathogenic microorganisms (2011) Proteomics, Glycomics and Antigenicity of BSL3 and BSL4 Agents, pp. 212-219. , Jiri Stulik J, Toman R, Butaye P and Ulrich R (editors), Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA
  • Mellmann, A., Cloud, J., Maier, T., Keckevoet, U., Ramminger, I., Evaluation of matrix-assisted laser desorption ionization-time-offlight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria (2008) J Clin Microbiol, 46, pp. 1946-1954
  • Lasch, P., Naumann, D., (2015) Infrared Spectroscopy in Microbiology. Encyclopedia of Analytical Chemistry, pp. 1-32. , Berlin, Germany: John Wiley & Sons, Ltd
  • Lasch, P., (2017) Microbems: A Matlab Toolbox for Analysis of Microbial MALDI-TOF Mass Spectra, , www.microbe-ms.com
  • Goris, J., Konstantinidis, K.T., Klappenbach, J.A., Coenye, T., Vandamme, P., DNA-DNA hybridization values and their relationship to whole-genome sequence similarities (2007) Int J Syst Evol Microbiol, 57, pp. 81-91
  • Vandamme, P., Dawyndt, P., Classification and identification of the Burkholderia cepacia complex: Past, present and future (2011) Syst Appl Microbiol, 34, pp. 87-95
  • De Carvalho, C.C., Caramujo, M.J., Bacterial diversity assessed by cultivation-based techniques shows predominance of Staphylococccus species on coins collected in Lisbon and Casablanca (2014) FEMS Microbiol Ecol, 88, pp. 26-37
  • Vauterin, L., Yang, P., Swings, J., Utilization of fatty acid methyl esters for the differentiation of new Xanthomonas species (1996) Int J Syst Bacteriol, 46, pp. 298-304
  • Henry, D.A., Mahenthiralingam, E., Vandamme, P., Coenye, T., Speert, D.P., Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex (2001) J Clin Microbiol, 39, pp. 1073-1078
  • Ligozzi, M., Bernini, C., Bonora, M.G., De Fatima, M., Zuliani, J., Evaluation of the VITEK 2 system for identification and antimicrobial susceptibility testing of medically relevant gram-positive cocci (2002) J Clin Microbiol, 40, pp. 1681-1686

Citas:

---------- APA ----------
Martina, P., Leguizamon, M., Prieto, C.I., Sousa, S.A., Montanaro, P., Draghi, W.O., Stämmler, M.,..., Bosch, A. (2018) . Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils. International Journal of Systematic and Evolutionary Microbiology, 68(1), 14-20.
http://dx.doi.org/10.1099/ijsem.0.002293
---------- CHICAGO ----------
Martina, P., Leguizamon, M., Prieto, C.I., Sousa, S.A., Montanaro, P., Draghi, W.O., et al. "Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils" . International Journal of Systematic and Evolutionary Microbiology 68, no. 1 (2018) : 14-20.
http://dx.doi.org/10.1099/ijsem.0.002293
---------- MLA ----------
Martina, P., Leguizamon, M., Prieto, C.I., Sousa, S.A., Montanaro, P., Draghi, W.O., et al. "Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils" . International Journal of Systematic and Evolutionary Microbiology, vol. 68, no. 1, 2018, pp. 14-20.
http://dx.doi.org/10.1099/ijsem.0.002293
---------- VANCOUVER ----------
Martina, P., Leguizamon, M., Prieto, C.I., Sousa, S.A., Montanaro, P., Draghi, W.O., et al. Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils. Int. J. Syst. Evol. Microbiol. 2018;68(1):14-20.
http://dx.doi.org/10.1099/ijsem.0.002293