Artículo

Conforte, V.P.; Malamud, F.; Yaryura, P.M.; Toum Terrones, L.; Torres, P.S.; De Pino, V.; Chazarreta, C.N.; Gudesblat, G.E.; Castagnaro, A.P.; R. Marano, M.; Vojnov, A.A."The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis" (2019) Molecular Plant Pathology. 20(4):589-598
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Abstract:

Citrus canker is an important disease of citrus, whose causal agent is the bacterium Xanthomonas citri ssp. citri (Xcc). In previous studies, we found a group of Xcc mutants, generated by the insertion of the Tn5 transposon, which showed impaired ability to attach to an abiotic substrate. One of these mutants carries the Tn5 insertion in hupB, a gene encoding a bacterial histone-like protein, homologue to the β-subunit of the Heat-Unstable (HU) nucleoid protein of Escherichia coli. These types of protein are necessary to maintain the bacterial nucleoid organization and the global regulation of gene expression. Here, we characterized the influence of the mutation in hupB regarding Xcc biofilm formation and virulence. The mutant strain hupB was incapable of swimming in soft agar, whereas its complemented strain partially recovered this phenotype. Electron microscope imaging revealed that impaired motility of hupB was a consequence of the absence of the flagellum. Comparison of the expression of flagellar genes between the wild-type strain and hupB showed that the mutant exhibited decreased expression of fliC (encoding flagellin). The hupB mutant also displayed reduced virulence compared with the wild-type strain when they were used to infect Citrus lemon plants using different infection methods. Our results therefore show that the histone-like protein HupB plays an essential role in the pathogenesis of Xcc through the regulation of biofilm formation and biosynthesis of the flagellum. © 2018 The Authors. Molecular Plant Pathology published by BSPP and John Wiley & Sons Ltd

Registro:

Documento: Artículo
Título:The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis
Autor:Conforte, V.P.; Malamud, F.; Yaryura, P.M.; Toum Terrones, L.; Torres, P.S.; De Pino, V.; Chazarreta, C.N.; Gudesblat, G.E.; Castagnaro, A.P.; R. Marano, M.; Vojnov, A.A.
Filiación:Instituto de Ciencia y Tecnología Dr. César Milstein, Fundación Pablo Cassará, CONICET, Saladillo 2468, Ciudad de Buenos Aires, C1440FFX, Argentina
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, Campus Migueletes, 25 de Mayo y Francia, General San Martín, Provincia de Buenos Aires B1650HMN, Argentina
Centro de Investigaciones y Transferencia de Villa María CONICET, Universidad de Villa María, Carlos Pellegrini 211, Villa María, Córdoba X5900FSE, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Instituto de Biodiversidad y Biología Experimental y Aplicada, CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Buenos Aires, C1428EGA, Argentina
Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Estación Experimental Agroindustrial Obispo Colombres (EEAOC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. William Cross 3150, Las Talitas, Tucumán C.P. T4101XAC, Argentina
Instituto de Biología Molecular y Celular de Rosario, Departamento de Microbiología, Facultad de Ciencias, Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, Santa Fé S2002LRK, Argentina
Palabras clave:biofilm; citrus; flagella; histone-like protein; HU; pathogenicity; Xanthomonas
Año:2019
Volumen:20
Número:4
Página de inicio:589
Página de fin:598
DOI: http://dx.doi.org/10.1111/mpp.12777
Handle:http://hdl.handle.net/20.500.12110/paper_14646722_v20_n4_p589_Conforte
Título revista:Molecular Plant Pathology
Título revista abreviado:Mol. Plant Pathol.
ISSN:14646722
CODEN:MPPAF
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14646722_v20_n4_p589_Conforte

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Citas:

---------- APA ----------
Conforte, V.P., Malamud, F., Yaryura, P.M., Toum Terrones, L., Torres, P.S., De Pino, V., Chazarreta, C.N.,..., Vojnov, A.A. (2019) . The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis. Molecular Plant Pathology, 20(4), 589-598.
http://dx.doi.org/10.1111/mpp.12777
---------- CHICAGO ----------
Conforte, V.P., Malamud, F., Yaryura, P.M., Toum Terrones, L., Torres, P.S., De Pino, V., et al. "The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis" . Molecular Plant Pathology 20, no. 4 (2019) : 589-598.
http://dx.doi.org/10.1111/mpp.12777
---------- MLA ----------
Conforte, V.P., Malamud, F., Yaryura, P.M., Toum Terrones, L., Torres, P.S., De Pino, V., et al. "The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis" . Molecular Plant Pathology, vol. 20, no. 4, 2019, pp. 589-598.
http://dx.doi.org/10.1111/mpp.12777
---------- VANCOUVER ----------
Conforte, V.P., Malamud, F., Yaryura, P.M., Toum Terrones, L., Torres, P.S., De Pino, V., et al. The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis. Mol. Plant Pathol. 2019;20(4):589-598.
http://dx.doi.org/10.1111/mpp.12777