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We analyzed the influence of the redox global regulator Anr on the accumulation of poly(3-hydroxybutyrate) (PHB) in Pseudomonas extremaustralis. Anr regulates a set of genes in the aerobic-anaerobic transition including genes involved in nitrate reduction and arginine fermentation. An anr mutant was constructed using PCR-based strategies. The wild-type strain was able to grow in both microaerobic and anaerobic conditions using nitrate as the terminal electron acceptor while the mutant strain was unable to grow under anaerobic conditions. In bioreactor cultures, PHB content in the wild-type strain was higher in microaerobic and anaerobic cultures compared with highly aerated cultures. The mutant strain showed decreased PHB levels in both aerobic and microaerobic conditions compared with the wild-type strain. Inactivation of anr led to decreased expression of phaC and phaR genes as demonstrated in real-time RT-PCR experiments. Associated with the PHB gene region, two putative binding sites for Anr were found that, in line with the phenotype observed in bioreactor cultures, suggest a role of this regulator in PHB biosynthesis. copyright © 2010 S. Karger AG, Basel.


Documento: Artículo
Título:Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis
Autor:Tribelli, P.M.; Méndez, B.S.; López, N.I.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Buenos Aires C1428EGA, Argentina
Palabras clave:Anr; Microaerobiosis; Poly(3-hydroxybutyrate); Pseudomonas; nitrate; poly(3 hydroxybutyric acid); aerobic bacterium; aerobic metabolism; anaerobic bacterium; Anr gene; article; bacterial gene; bacterial genetics; bacterial growth; bacterial strain; bacterium culture; binding site; bioreactor; controlled study; electron; gene control; gene expression; gene inactivation; gene location; genetic association; mutant; nonhuman; nucleotide sequence; oxygen sensitive global regulator; phaC gene; phaR gene; phenotype; polymerase chain reaction; Pseudomonas; Pseudomonas extremaustralis; real time polymerase chain reaction; wild type; Bacterial Proteins; Base Sequence; Bioreactors; DNA-Binding Proteins; Gene Expression Regulation; Hydroxybutyrates; Molecular Sequence Data; Mutation; Oxygen; Polyesters; Promoter Regions, Genetic; Pseudomonas; Sequence Alignment; Pseudomonas
Página de inicio:180
Página de fin:188
Título revista:Journal of Molecular Microbiology and Biotechnology
Título revista abreviado:J. Mol. Microbiol. Biotechnol.
CAS:nitrate, 14797-55-8; poly(3 hydroxybutyric acid), 26063-00-3; Bacterial Proteins; DNA-Binding Proteins; Hydroxybutyrates; Oxygen, 7782-44-7; Polyesters; poly-beta-hydroxybutyrate, 26063-00-3


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---------- APA ----------
Tribelli, P.M., Méndez, B.S. & López, N.I. (2011) . Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis. Journal of Molecular Microbiology and Biotechnology, 19(4), 180-188.
---------- CHICAGO ----------
Tribelli, P.M., Méndez, B.S., López, N.I. "Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis" . Journal of Molecular Microbiology and Biotechnology 19, no. 4 (2011) : 180-188.
---------- MLA ----------
Tribelli, P.M., Méndez, B.S., López, N.I. "Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis" . Journal of Molecular Microbiology and Biotechnology, vol. 19, no. 4, 2011, pp. 180-188.
---------- VANCOUVER ----------
Tribelli, P.M., Méndez, B.S., López, N.I. Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis. J. Mol. Microbiol. Biotechnol. 2011;19(4):180-188.