Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

We analyzed the influence of the redox global regulator Anr on the accumulation of poly(3-hydroxybutyrate) (PHB) in Pseudomonas extremaustralis. Anr regulates a set of genes in the aerobic-anaerobic transition including genes involved in nitrate reduction and arginine fermentation. An anr mutant was constructed using PCR-based strategies. The wild-type strain was able to grow in both microaerobic and anaerobic conditions using nitrate as the terminal electron acceptor while the mutant strain was unable to grow under anaerobic conditions. In bioreactor cultures, PHB content in the wild-type strain was higher in microaerobic and anaerobic cultures compared with highly aerated cultures. The mutant strain showed decreased PHB levels in both aerobic and microaerobic conditions compared with the wild-type strain. Inactivation of anr led to decreased expression of phaC and phaR genes as demonstrated in real-time RT-PCR experiments. Associated with the PHB gene region, two putative binding sites for Anr were found that, in line with the phenotype observed in bioreactor cultures, suggest a role of this regulator in PHB biosynthesis. copyright © 2010 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis
Autor:Tribelli, P.M.; Méndez, B.S.; López, N.I.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Buenos Aires C1428EGA, Argentina
Palabras clave:Anr; Microaerobiosis; Poly(3-hydroxybutyrate); Pseudomonas; nitrate; poly(3 hydroxybutyric acid); aerobic bacterium; aerobic metabolism; anaerobic bacterium; Anr gene; article; bacterial gene; bacterial genetics; bacterial growth; bacterial strain; bacterium culture; binding site; bioreactor; controlled study; electron; gene control; gene expression; gene inactivation; gene location; genetic association; mutant; nonhuman; nucleotide sequence; oxygen sensitive global regulator; phaC gene; phaR gene; phenotype; polymerase chain reaction; Pseudomonas; Pseudomonas extremaustralis; real time polymerase chain reaction; wild type; Bacterial Proteins; Base Sequence; Bioreactors; DNA-Binding Proteins; Gene Expression Regulation; Hydroxybutyrates; Molecular Sequence Data; Mutation; Oxygen; Polyesters; Promoter Regions, Genetic; Pseudomonas; Sequence Alignment; Pseudomonas
Año:2011
Volumen:19
Número:4
Página de inicio:180
Página de fin:188
DOI: http://dx.doi.org/10.1159/000320261
Título revista:Journal of Molecular Microbiology and Biotechnology
Título revista abreviado:J. Mol. Microbiol. Biotechnol.
ISSN:14641801
CODEN:JMMBF
CAS:nitrate, 14797-55-8; poly(3 hydroxybutyric acid), 26063-00-3; Bacterial Proteins; DNA-Binding Proteins; Hydroxybutyrates; Oxygen, 7782-44-7; Polyesters; poly-beta-hydroxybutyrate, 26063-00-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14641801_v19_n4_p180_Tribelli

Referencias:

  • Anjana, R., Williams, H., The effects of the anr mutation on the aerobic respiratory chain of Pseudomonas aeruginosa (1997) FEMS Microbiol Lett, 156, pp. 227-232
  • Ayub, N.D., Pettinari, M.J., Méndez, B.S., López, N.I., Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective ß-ketothiolase gene (2006) FEMS Microbiol Lett, 264, pp. 125-131
  • Ayub, N.D., Pettinari, M.J., Méndez, B.S., López, N.I., The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island (2007) Plasmid, 58, pp. 240-248
  • Ayub, N.D., Tribelli, P.M., López, N.I., Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation (2009) Extremophiles, 13, pp. 59-66
  • Blumer, C., Haas, D., Iron regulation of the hcnABC genes encoding hydrogen cyanide synthase depends on the anaerobic regulator ANR rather than on the global activator GacA in Pseudomonas fluorescens CHAO (2000) Microbiology, 146, pp. 2417-2424
  • Boes, N., Shreiber, K., Schobert, M., SpoT-triggered stringent response control usp gene expression in Pseudomonas aeruginosa (2008) J Bacteriol, 190, pp. 7189-7199
  • Braunegg, G., Sonnleitner, B., Lafferty, R., A rapid gas chromatographic method for the determination of poly- β -hydroxybutyric acid in microbial biomass (1978) Eur J Appl Microbiol Biotechnol, 6, pp. 29-37
  • Crack, J., Green, J., Thomson, A., Mechanism of oxygen sensing by the bacterial transcription factor fumarate-nitrate reduction (FNR) (2003) J Biol Chem, 279, pp. 9278-9286
  • Dos Santos, M., Heim, S., Moore, E.R., Strätz, M., Timmis, K.M., Insights into the genomic basis of niche specificity of Pseudomonas putida KT2440 (2004) Environ Ecol, 6, pp. 1264-1286
  • Fu, H.A., Luchi, S., Lin, E.C., The requirement of ArcA and Fnr for peak expression of the cyd operon in Escherichia coli under microaerobic conditions (1991) Mol Gen Genet, 226, pp. 209-213
  • Galimand, M., Gamper, M., Zimmermann, A., Haas, D., Positive FNR-like control of anaerobic arginine degradation and nitrate respiration in Pseudomonas aeruginosa (1991) J Bacteriol, 173, pp. 1598-1606
  • Gerhard, P., Murray, R.N., Costilow, N., Nester, W., Wood, N., Krieg, N.R., Phillips, G., (1981) Manual of Method of General Bacteriology, pp. 409-443. , Washington American Society for microbiology
  • Gunsalus, R.P., Control of electron flow in Escherichia coli : CCoordinated transcription of respiratory pathway genes (1992) J Bacteriol, 174, pp. 7069-7074
  • Hazer, B., Steinbüchel, A., Increased diversification of polyhydroxyalkanoates by modification reactions for industrial and medical applications (2007) Appl Microbiol Biotechnol, 74, pp. 1-12
  • Hong, C.S., Kuroda, A., Ikeda, T., Takiguchi, N., Ohtake, H., Kato, J., The aerotaxis transducer gene aer, but not aer -2, is transcriptionally regulated by the anaerobic regulator ANR in Pseudomonas aeruginosa (2004) J Biosci Bioeng, 97, pp. 184-190
  • Huisman, G.W., Wonink, E., De Koning, G., Preusting, H., Witholt, B., Synthesis of poly(3-hydroxyalkanoates) by mutant and recombinant Pseudomonas strains (1992) Appl Microbiol Biotechnol, 38, pp. 1-5
  • Kadouri, D., Jurkevitch, E., Okon, Y., Castro-Sowinski, S., Ecological and agricultural significance of bacterial polyhydroxyalkanoates (2005) Crit Rev Microbiol, 31, pp. 55-67
  • Kessler, B., Palleroni, N.J., Taxonomic implications of synthesis of poly-beta-hydroxybutyrate and other poly-beta-hydroxyalkanoates by aerobic pseudomonads (2000) Int J Syst Evol Microbiol, 50, pp. 711-713
  • Kovács, Á.T., Rákhely, G., Balogh, J., Maróti, G., Fülöp, A., Kovács, K.L., Anaerobic regulation of hydrogenase transcription in different bacteria (2005) Biochem Soc Trans, 33, pp. 36-38
  • Krieger, R., Rompf, A., Schobert, M., Jahn, D., The Pseudomonas aeruginosa hemA promoter is regulated by Anr, Dnr, NarL and Integration Host Factor (2002) Mol Genet Genomics, 267, pp. 409-417
  • Larionov, A., Krause, A., Miller, W., A standard curve based method for relative real time PCR data processing (2005) BMC, 6, p. 62
  • Laville, J., Blumer, C., Von Schoetter, C., Gaia, V., Dèfago, G., Keel, C., Hass, D., Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHAO (1998) J Bacteriol, 180, pp. 3187-3196
  • Lee, S.H., Lee, S.Y., Park, B.C., Cell surface display of lipase in Pseudomonas putida KT2442 using OprF as an anchoring motif and its biocatalytic applicationsAppl (2005) Environ Microbiol, 71, pp. 8581-8586
  • Link, A.J., Phillips, D., Church, G.M., Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli : AApplication to open reading frame characterization (1997) J Bacteriol, 179, pp. 6228-6237
  • López, N., Pettinari, M.J., Stackebrandt, E., Tribelli, P.M., Pötter, M., Steinbüchel, A., Méndez, B., Pseudomonas extremaustralis sp. nov. A poly(3-hydroxybutyrate) producer isolated from an Antarctic environment (2009) Curr Microbiol, 59, pp. 514-519
  • López, N.I., Floccari, M.E., Garcia, A.F., Steinbüchel, A., Mendez, B.S., Effect of poly-3-hydroxybutyrate content on the starvation survival of bacteria in natural waters (1995) FEMS Microbiol Ecol, 16, pp. 95-101
  • Matsusaki, H., Manji, S., Taguchi, K., Kato, M., Fukui, T., Yoshiharu, D., Cloning and molecular analysis of the poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in Pseudomonas sp. Strain 61-3 (1998) J Bacteriol, 180, pp. 6459-6467
  • Mazoch, J., Kucera, I., Control of gene expression by FNR-like proteins in facultative anaerobic bacteria (2002) Folia Microbiol, 47, pp. 95-103
  • Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S., Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis (2006) Appl Environ Microbiol, 72, pp. 2614-2620
  • Peralta-Gil, M., Segura, D., Guzmán, J., Servín-González, L., Espín, G., Expression of the Azotobacter vinelandii poly-hydroxybutyrate biosynthetic phbBAC operon is driven by two overlapping promoters and is dependent on the transcriptional activator PhbR (2002) J Bacteriol, 184, pp. 5672-5677
  • Pettinari, M.J., Vázquez, G., Silberschmidt, D., Rehm, D., Steinbüchel, A., Méndez, B., Poly (3-hydroxybutyrate) (PHB) synthesis genes in Azotobacter sp. strain FA8 (2001) Appl Environ Microbiol, 67, pp. 5331-5334
  • Pham, T., Webb, J., Rehm, B., The role of polyhydroxyalkanoate biosynthesis by Pseudomonas aeruginosa in rhamnolipid and alginate production as well as stress tolerance and biofilm formation (2004) Microbiology, 150, pp. 3405-3413
  • Povolo, S., Casella, S., A critical role for aniA in energycarbon flux and symbiotic nitrogen fixation in Sinorhizobium meliloti (2000) Arch Microbiol, 174, pp. 42-49
  • Raiger Iustman, L., Ruiz, J., The alternative sigma factor αs , affects polyhydroxyalkanoate (PHA) metabolism in Pseudomonas putida (2008) FEMS Microbiol Lett, 284, pp. 216-224
  • Rompf, A., Hungerer, C., Hoffmann, T., Lindenmeyer, M., Römling, U., Groß, U., Doss, M.O., Jahn, D., Regulation of Pseudomonas aeruginosa hemF and hemN by the dual action of the redox response regulators Anr and Dnr (1998) Mol Microbiol, 129, pp. 985-997
  • Ruiz, J.A., López, N.I., Méndez, B.S., RpoS gene expression in carbon starved cultures of the polyhydroxyalkanoate accumulating species Pseudomonas oleovorans (2004) Curr Microbiol, 48, pp. 396-400
  • Sawers, R.G., Identification and molecular characterization of transcriptional regulator from Pseudomonas aeruginosa PAO1 exhibiting structural and functional similarity to the FNR protein of Escherichia coli (1991) Mol Microbiol, 5, pp. 1469-1481
  • Setubal, J., Dos Santos, P., Goldman, B.S., Genome sequence of Azotobacter vinelandii , an obligate aerobe specialized to support diverse anaerobic metabolic processes (2009) J Bacteriol, 191, pp. 4534-4545
  • Soupene, E., Van Heeswijk, W., Plumbridge, S., Physiological studies of Escherichia coli MG1655: Defects and apparent cross-regulation gene expression (2003) J Bacteriol, 185, pp. 5611-5626
  • Ugidos, A., Morales, G., Rial, E., Williams, H.D., Rojo, F., The coordinate regulation of multiple terminal oxidases by the Pseudomonas putida ANR global regulator (2008) Environ Microbiol, 10, pp. 1690-1702
  • Winteler, H.V., Haas, D., The homologous regulators ANR of Pseudomonas aeruginosa and FNR of Escherichia coli have overlapping but distinct specificities for anaerobically inducible promoters (1996) Microbiology, 142, pp. 685-693
  • Wu, G., Moir, A.J.G., Sawers, G., Hill, S., Poole, R.K., Biosynthesis of poly-ß-hydroxybutyrate (PHB) is controlled by CydR (Fnr) in the obligate aerobe Azotobacter vinelandii (2001) FEMS Microbiol Lett, 194, pp. 215-220
  • Yan, Y., Jian, Y., Yuetan, D., Nitrogen fixation island and rhizosphere competent traits in the genome of root-associated Pseudomonas stutzeri A1501 (2008) PNAS, 105, pp. 7564-7569
  • Yoon, S.S., Hennigan, R.F., Hilliard, G.M., Pseudomonas aeruginosa anaerobic respiration in biofilms: Relationships to cystic fibrosis pathogenesis (2002) Dev Cell, 3, pp. 593-603

Citas:

---------- APA ----------
Tribelli, P.M., Méndez, B.S. & López, N.I. (2011) . Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis. Journal of Molecular Microbiology and Biotechnology, 19(4), 180-188.
http://dx.doi.org/10.1159/000320261
---------- CHICAGO ----------
Tribelli, P.M., Méndez, B.S., López, N.I. "Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis" . Journal of Molecular Microbiology and Biotechnology 19, no. 4 (2011) : 180-188.
http://dx.doi.org/10.1159/000320261
---------- MLA ----------
Tribelli, P.M., Méndez, B.S., López, N.I. "Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis" . Journal of Molecular Microbiology and Biotechnology, vol. 19, no. 4, 2011, pp. 180-188.
http://dx.doi.org/10.1159/000320261
---------- VANCOUVER ----------
Tribelli, P.M., Méndez, B.S., López, N.I. Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis. J. Mol. Microbiol. Biotechnol. 2011;19(4):180-188.
http://dx.doi.org/10.1159/000320261