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Abstract:

ArcA is a global regulator that switches on the expression of fermentation genes and represses the aerobic pathways when Escherichia coli enters low oxygen growth conditions. The metabolic profile of E. coli CT1062 (ΔarcA)and CT1061 (arcA2) grown in microaerobiosis with glycerol as carbon source were determined and compared with E. coli K1060, the arcA+ parent strain. Both arcA mutants achieved higher biomass yields than the wild-type strain. The production of acetate, formate, lactate, pyruvate, succinate and ethanol were determined in the supernatants of cultures grown on glycerol under microaerobic conditions for 48 h. The yield of extracellular metabolites on glycerol showed lower acid and higher ethanol values for the mutants. The ethanol/acetate ratio was 0.87 for the parent strain, 2.01 for CT1062, and 12.51 for CT1061. Accordingly, the NADH/NAD+ ratios were 0.18, 0.63, and 0.97, respectively. The extracellular succinate yield followed a different pattern, with yield values of 0.164 for K1060, 0.442 for CT1062 and 0.214 for CT1061. The dissimilarities observed can be attributed to the different effects exerted by the deletion and point mutations in a global regulator. Copyright © 2008 S. Karger AG.

Registro:

Documento: Artículo
Título:Escherichia coli arcA mutants: Metabolic profile characterization of microaerobic cultures using glycerol as a carbon source
Autor:Nikel, P.I.; Pettinari, M.J.; Ramírez, M.C.; Galvagno, M.A.; Méndez, B.S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, San Martín, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Av. Int. Güiraldes 2160, C1428EGA Buenos Aires, Argentina
Palabras clave:ArcA; E. coli arcA; Escherichia coli; acetic acid; alcohol; carbon; formic acid; glycerol; lactic acid; pyruvic acid; succinic acid; article; bacterial metabolism; bacterial strain; bacterium culture; carbon source; controlled study; gene deletion; gene mutation; nonhuman; Aerobiosis; Bacterial Outer Membrane Proteins; Carbon; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Glycerol; Oxygen; Oxygen Consumption; Repressor Proteins; Arca; Escherichia coli
Año:2008
Volumen:15
Número:1
Página de inicio:48
Página de fin:54
DOI: http://dx.doi.org/10.1159/000111992
Título revista:Journal of Molecular Microbiology and Biotechnology
Título revista abreviado:J. Mol. Microbiol. Biotechnol.
ISSN:14641801
CODEN:JMMBF
CAS:acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; alcohol, 64-17-5; carbon, 7440-44-0; formic acid, 64-18-6, 71-47-6; glycerol, 56-81-5; lactic acid, 113-21-3, 50-21-5; pyruvic acid, 127-17-3, 19071-34-2, 57-60-3; succinic acid, 110-15-6; arcA protein, E coli; Bacterial Outer Membrane Proteins; Carbon, 7440-44-0; Escherichia coli Proteins; Glycerol, 56-81-5; Oxygen, 7782-44-7; Repressor Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14641801_v15_n1_p48_Nikel

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Citas:

---------- APA ----------
Nikel, P.I., Pettinari, M.J., Ramírez, M.C., Galvagno, M.A. & Méndez, B.S. (2008) . Escherichia coli arcA mutants: Metabolic profile characterization of microaerobic cultures using glycerol as a carbon source. Journal of Molecular Microbiology and Biotechnology, 15(1), 48-54.
http://dx.doi.org/10.1159/000111992
---------- CHICAGO ----------
Nikel, P.I., Pettinari, M.J., Ramírez, M.C., Galvagno, M.A., Méndez, B.S. "Escherichia coli arcA mutants: Metabolic profile characterization of microaerobic cultures using glycerol as a carbon source" . Journal of Molecular Microbiology and Biotechnology 15, no. 1 (2008) : 48-54.
http://dx.doi.org/10.1159/000111992
---------- MLA ----------
Nikel, P.I., Pettinari, M.J., Ramírez, M.C., Galvagno, M.A., Méndez, B.S. "Escherichia coli arcA mutants: Metabolic profile characterization of microaerobic cultures using glycerol as a carbon source" . Journal of Molecular Microbiology and Biotechnology, vol. 15, no. 1, 2008, pp. 48-54.
http://dx.doi.org/10.1159/000111992
---------- VANCOUVER ----------
Nikel, P.I., Pettinari, M.J., Ramírez, M.C., Galvagno, M.A., Méndez, B.S. Escherichia coli arcA mutants: Metabolic profile characterization of microaerobic cultures using glycerol as a carbon source. J. Mol. Microbiol. Biotechnol. 2008;15(1):48-54.
http://dx.doi.org/10.1159/000111992