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Abstract:

Supramolecular multilayer structures comprised of glucose oxidase (GOx), and Os complex derivatised poly(allylamine) (PAH-Os) have been built by alternate layer-by-layer (LBL) electrostatic adsorption in a self-assembly process. The resulting modified electrodes with integrated mediator were tested as reagentless glucose biosensors. The enzyme kinetic parameters and the surface concentration of "wired" enzyme ΓE have been obtained by analysis of the catalytic current dependence on glucose concentrations for the ping-pong mechanism of glucose oxidation. An average osmium volume concentration was estimated by integration of the redox charge in the absence of glucose and the ellipsometric thickness. The total enzyme surface concentration was measured with a quartz crystal microbalance (QCM) during each adsoption step and the fraction of "wired" enzyme and the bimolecular rate constant for FADH2 oxidation by the redox polymer for the different multilayers. The catalytic current increases with the number of LBL layers because the increase in the enzyme loading while the efficiency of enzyme FADH2 oxidation by the Os redox polymer, except for the first dipping cycle remains almost constant at about 2 × 104 M-1 s-1. © The Owner Societies 2005.

Registro:

Documento: Artículo
Título:Supramolecular multilayer structures of wired redox enzyme electrodes
Autor:Calvo, E.J.; Danilowicz, C.B.; Wolosiuk, A.
Filiación:INQUIMAE, Depto. de Quim. Inorg., Analitica y., Fac. de Ciencias Exactas y Naturales, Pabellón 2 Cd. Universitaria, AR-1428 Buenos Aires, Argentina
Physics Department, Harvard University, 17 Oxford St., Cambridge, MA 02 138, United States
Dept. of Mat. Sci. and Engineering, University of Illinois, Urbana-Champaign, IL 61801, United States
Palabras clave:glucose oxidase; polyallylamine; polyamine; article; biocatalysis; chemistry; electrode; genetic procedures; kinetics; oxidation reduction reaction; Biocatalysis; Biosensing Techniques; Electrodes; Glucose Oxidase; Kinetics; Oxidation-Reduction; Polyamines
Año:2005
Volumen:7
Número:8
Página de inicio:1800
Página de fin:1806
DOI: http://dx.doi.org/10.1039/b500528k
Título revista:Physical Chemistry Chemical Physics
Título revista abreviado:Phys. Chem. Chem. Phys.
ISSN:14639076
CODEN:PPCPF
CAS:glucose oxidase, 9001-37-0; Glucose Oxidase, 1.1.3.4; Polyamines; polyallylamine, 30551-89-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14639076_v7_n8_p1800_Calvo

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Citas:

---------- APA ----------
Calvo, E.J., Danilowicz, C.B. & Wolosiuk, A. (2005) . Supramolecular multilayer structures of wired redox enzyme electrodes. Physical Chemistry Chemical Physics, 7(8), 1800-1806.
http://dx.doi.org/10.1039/b500528k
---------- CHICAGO ----------
Calvo, E.J., Danilowicz, C.B., Wolosiuk, A. "Supramolecular multilayer structures of wired redox enzyme electrodes" . Physical Chemistry Chemical Physics 7, no. 8 (2005) : 1800-1806.
http://dx.doi.org/10.1039/b500528k
---------- MLA ----------
Calvo, E.J., Danilowicz, C.B., Wolosiuk, A. "Supramolecular multilayer structures of wired redox enzyme electrodes" . Physical Chemistry Chemical Physics, vol. 7, no. 8, 2005, pp. 1800-1806.
http://dx.doi.org/10.1039/b500528k
---------- VANCOUVER ----------
Calvo, E.J., Danilowicz, C.B., Wolosiuk, A. Supramolecular multilayer structures of wired redox enzyme electrodes. Phys. Chem. Chem. Phys. 2005;7(8):1800-1806.
http://dx.doi.org/10.1039/b500528k