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Abstract:

Summary: Translation efficiency of viral mRNAs is a key factor defining both cytopathogenicity and virulence of viruses, which are entirely dependent on the cellular translation machinery to synthesize their proteins. This dependence has led them to develop different translational reprogramming strategies to ensure viral mRNAs can effectively compete with cellular mRNAs. Junin virus (JUNV) is a member of the family Arenaviridae, whose mRNAs are capped but not polyadenylated. In this work we evaluated the relevance to JUNV replication of the main components of the eIF4F complex: eIF4A, eIF4GI and eIF4E. We found the viral nucleoprotein (N) of JUNV colocalized with eIF4A and eIF4GI but not with eIF4E. Moreover, N could be immunoprecipitated in association with eIF4A and eIF4GI but not with eIF4E. Accordingly, functional impairment of eIF4A as well as eIF4GI reduced JUNV multiplication. By contrast, inhibition of eIF4E did not show a significant effect on JUNV protein synthesis. A similar situation was observed for another two members of arenaviruses: Tacaribe (TCRV) and Pichinde (PICV) viruses. Finally, the nucleoproteins of JUNV, TCRV and PICV were able to interact with 7 methyl-guanosine (cap), suggesting that the independence of JUNV multiplication on eIF4E, the cap-binding protein, may be due to the replacement of this factor by N protein. © 2013 John Wiley & Sons Ltd.

Registro:

Documento: Artículo
Título:Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication
Autor:Linero, F.; Welnowska, E.; Carrasco, L.; Scolaro, L.
Filiación:Laboratorio De Virología, Dpto. Química Biológica, FCEyN, Universidad De Buenos Aires, Intendente Güiraldes 2160, Buenos Aires, Argentina
Centro De Biología Molecular 'Severo Ochoa', CSIC-UAM, Universidad Autónoma De Madrid, C/Nicolás Cabrera 1, Cantoblanco, 28049 Madrid, Spain
Palabras clave:2 morpholino 8 phenylchromone; initiation factor 4A; initiation factor 4E; initiation factor 4F; initiation factor 4G; messenger RNA; rapamycin; virus protein; amino terminal sequence; Argentine hemorrhagic fever; article; cellular distribution; comparative study; confocal microscopy; controlled study; cytoplasm; Encephalomyocarditis virus; immunoprecipitation; Junin virus; nonhuman; oxidative stress; Pichinde arenavirus; polyacrylamide gel electrophoresis; priority journal; protein secondary structure; protein synthesis; synthesis; translation initiation; Vero cell; virogenesis; virus culture; virus replication; Arenaviridae; Junin virus; Pichinde virus; Animals; Cercopithecus aethiops; Eukaryotic Initiation Factor-4A; Eukaryotic Initiation Factor-4E; Eukaryotic Initiation Factor-4G; Guanosine; Host-Pathogen Interactions; Junin virus; Nucleocapsid Proteins; Vero Cells; Virus Replication
Año:2013
Volumen:15
Número:10
Página de inicio:1766
Página de fin:1782
DOI: http://dx.doi.org/10.1111/cmi.12149
Título revista:Cellular Microbiology
Título revista abreviado:Cell. Microbiol.
ISSN:14625814
CODEN:CEMIF
CAS:2 morpholino 8 phenylchromone, 154447-36-6; rapamycin, 53123-88-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14625814_v15_n10_p1766_Linero

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Citas:

---------- APA ----------
Linero, F., Welnowska, E., Carrasco, L. & Scolaro, L. (2013) . Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication. Cellular Microbiology, 15(10), 1766-1782.
http://dx.doi.org/10.1111/cmi.12149
---------- CHICAGO ----------
Linero, F., Welnowska, E., Carrasco, L., Scolaro, L. "Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication" . Cellular Microbiology 15, no. 10 (2013) : 1766-1782.
http://dx.doi.org/10.1111/cmi.12149
---------- MLA ----------
Linero, F., Welnowska, E., Carrasco, L., Scolaro, L. "Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication" . Cellular Microbiology, vol. 15, no. 10, 2013, pp. 1766-1782.
http://dx.doi.org/10.1111/cmi.12149
---------- VANCOUVER ----------
Linero, F., Welnowska, E., Carrasco, L., Scolaro, L. Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication. Cell. Microbiol. 2013;15(10):1766-1782.
http://dx.doi.org/10.1111/cmi.12149