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Grandellis, C.; Giammaria, V.; Fantino, E.; Cerrudo, I.; Bachmann, S.; Santin, F.; Ulloa, R.M. "Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness" (2016) Functional and Integrative Genomics. 16(4):399-418
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Abstract:

Potato (Solanum tuberosum L.) tubers are an excellent staple food due to its high nutritional value. When the tuber reaches physiological competence, sprouting proceeds accompanied by changes at mRNA and protein levels. Potato tubers become a source of carbon and energy until sprouts are capable of independent growth. Transcript profiling of sprouts grown under continuous light or dark conditions was performed using the TIGR 10K EST Solanaceae microarray. The profiles analyzed show a core of highly expressed transcripts that are associated to the reactivation of growth. Under light conditions, the photosynthetic machinery was fully activated; the highest up-regulation was observed for the Rubisco activase (RCA), the glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the Photosystem II 22 kDa protein (CP22) genes, among others. On the other hand, sprouts exposed to continuous darkness elongate longer, and after extended darkness, synthesis of chloroplast components was repressed, the expression of proteases was reduced while genes encoding cysteine protease inhibitors (CPIs) and metallocarboxypeptidase inhibitors (MPIs) were strongly induced. Northern blot and RT-PCR analysis confirmed that MPI levels correlated with the length of the dark period; however, CPI expression was strong only after longer periods of darkness, suggesting a feedback loop (regulation mechanism) in response to dark-induced senescence. Prevention of cysteine protease activity in etiolated sprouts exposed to extended darkness could delay senescence until they emerge to light. © 2016, Springer-Verlag Berlin Heidelberg.

Registro:

Documento: Artículo
Título:Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness
Autor:Grandellis, C.; Giammaria, V.; Fantino, E.; Cerrudo, I.; Bachmann, S.; Santin, F.; Ulloa, R.M.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490 2nd piso, Buenos Aires, C1428ADN, Argentina
Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas (IBR-CONICET) Ocampo y Esmeralda, Rosario, Argentina
Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA), Universidad de Buenos Aires, and Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Av. San Martín 4453, Buenos Aires, C1417DSE, Argentina
Instituto de Biotecnología (IB), Universidad Nacional de Hurlingham, Av. Vergara 2222, Villa Tesei, Hurlingham, Buenos Aires, B1688GEZ, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Light/dark conditions; Protease inhibitors; Solanum tuberosum; TIGR 10K microarrays; Tuber sprouting; chloroplast protein; cysteine proteinase; cysteine proteinase inhibitor; glyceraldehyde 3 phosphate dehydrogenase; metallocarboxypeptidase inhibitor; peptide hydrolase inhibitor; proton transporting adenosine triphosphate synthase; ribulosebisphosphate carboxylase; transcriptome; unclassified drug; cysteine proteinase inhibitor; plant protein; Article; cell metabolism; cell wall metabolism; correlational study; darkness; enzyme activity; feedback system; GAPDH gene; gene expression; genetic association; genetic procedures; light; microarray analysis; nonhuman; Northern blotting; photosystem II; plant gene; plant genome; plant growth; potato; priority journal; protein expression; protein metabolism; protein synthesis inhibition; RCA gene; reverse transcription polymerase chain reaction; senescence; sprout; sprouting; transcript profiling; upregulation; biosynthesis; darkness; gene expression regulation; genetics; growth, development and aging; photosynthesis; plant tuber; seedling; transcription initiation; Cysteine Proteinase Inhibitors; Darkness; Gene Expression Regulation, Plant; Light; Photosynthesis; Plant Proteins; Plant Tubers; Seedlings; Solanum tuberosum; Transcriptional Activation
Año:2016
Volumen:16
Número:4
Página de inicio:399
Página de fin:418
DOI: http://dx.doi.org/10.1007/s10142-016-0492-1
Título revista:Functional and Integrative Genomics
Título revista abreviado:Funct. Integr. Genomics
ISSN:1438793X
CODEN:FIGUB
CAS:cysteine proteinase, 37353-41-6; glyceraldehyde 3 phosphate dehydrogenase, 9001-50-7; proton transporting adenosine triphosphate synthase, 37205-63-3; ribulosebisphosphate carboxylase, 9027-23-0; Cysteine Proteinase Inhibitors; Plant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1438793X_v16_n4_p399_Grandellis

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Citas:

---------- APA ----------
Grandellis, C., Giammaria, V., Fantino, E., Cerrudo, I., Bachmann, S., Santin, F. & Ulloa, R.M. (2016) . Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness. Functional and Integrative Genomics, 16(4), 399-418.
http://dx.doi.org/10.1007/s10142-016-0492-1
---------- CHICAGO ----------
Grandellis, C., Giammaria, V., Fantino, E., Cerrudo, I., Bachmann, S., Santin, F., et al. "Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness" . Functional and Integrative Genomics 16, no. 4 (2016) : 399-418.
http://dx.doi.org/10.1007/s10142-016-0492-1
---------- MLA ----------
Grandellis, C., Giammaria, V., Fantino, E., Cerrudo, I., Bachmann, S., Santin, F., et al. "Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness" . Functional and Integrative Genomics, vol. 16, no. 4, 2016, pp. 399-418.
http://dx.doi.org/10.1007/s10142-016-0492-1
---------- VANCOUVER ----------
Grandellis, C., Giammaria, V., Fantino, E., Cerrudo, I., Bachmann, S., Santin, F., et al. Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness. Funct. Integr. Genomics. 2016;16(4):399-418.
http://dx.doi.org/10.1007/s10142-016-0492-1