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Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature. These responses are associated with cold hardiness, but their underlying mechanisms remain largely unknown. Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability. A combined microscopy and biochemical approach was applied to study actively growing leaves of winter (ProINTA-Pincén) and spring (Buck-Patacón) wheat developed under constant warm (25 °C) or cool (5 °C) temperature. Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants. They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose. These effects were more pronounced in the winter cultivar (Pincén). Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf. This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C. The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening. New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses. © 2018 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands


Documento: Artículo
Título:Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves
Autor:Lorenzo, M.; Pinedo, M.L.; Equiza, M.A.; Fernández, P.V.; Ciancia, M.; Ganem, D.G.; Tognetti, J.A.
Filiación:INTA, Unidad Integrada Balcarce, Balcarce, Buenos Aires, Argentina
Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata-CONICET, Mar del Plata, Argentina
Department of Renewable Resources, University of Alberta, Edmonton, Canada
Cátedra de Química de Biomoléculas, Departamento de Biología Aplicada y Alimentos, Facultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
CIHIDECAR-CONICET, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Research Member of the National Research Council of Argentina (CONICET), Ciudad Autónoma de Buenos Aires, Argentina
Laboratorio de Fisiología Vegetal, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Balcarce, Buenos Aires, Argentina
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC), La Plata, Buenos Aires, Argentina
Palabras clave:Cell elongation; cell wall; cold hardiness; hemicellulose; peroxidase; wheat; anatomy; cell component; cellulose; cold tolerance; cultivar; enzyme activity; grass; leaf morphology; low temperature; wheat; Poaceae; Triticum aestivum; hemicellulose; peroxidase; plant protein; polysaccharide; anatomy and histology; cell wall; cold; metabolism; physiology; plant leaf; season; wheat; Cell Wall; Cold Temperature; Peroxidase; Plant Leaves; Plant Proteins; Polysaccharides; Seasons; Triticum
Página de inicio:84
Página de fin:94
Título revista:Plant Biology
Título revista abreviado:Plant Biol.
CAS:hemicellulose, 63100-39-0, 63100-40-3, 9034-32-6; peroxidase, 9003-99-0; hemicellulose; Peroxidase; Plant Proteins; Polysaccharides


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---------- APA ----------
Lorenzo, M., Pinedo, M.L., Equiza, M.A., Fernández, P.V., Ciancia, M., Ganem, D.G. & Tognetti, J.A. (2019) . Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves. Plant Biology, 21, 84-94.
---------- CHICAGO ----------
Lorenzo, M., Pinedo, M.L., Equiza, M.A., Fernández, P.V., Ciancia, M., Ganem, D.G., et al. "Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves" . Plant Biology 21 (2019) : 84-94.
---------- MLA ----------
Lorenzo, M., Pinedo, M.L., Equiza, M.A., Fernández, P.V., Ciancia, M., Ganem, D.G., et al. "Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves" . Plant Biology, vol. 21, 2019, pp. 84-94.
---------- VANCOUVER ----------
Lorenzo, M., Pinedo, M.L., Equiza, M.A., Fernández, P.V., Ciancia, M., Ganem, D.G., et al. Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves. Plant Biol. 2019;21:84-94.