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Abstract:

Polyhydroxyalkanoates (PHAs) are highly reduced bacterial storage compounds that increase fitness in changing environments. We have previously shown that phaRBAC genes from the Antarctic bacterium Pseudomonas sp. 14-3 are located in a genomic island containing other genes probably related with its adaptability to cold environments. In this paper, Pseudomonas sp. 14-3 and its PHA synthase-minus mutant (phaC) were used to asses the effect of PHA accumulation on the adaptability to cold conditions. The phaC mutant was unable to grow at 10°C and was more susceptible to freezing than its parent strain. PHA was necessary for the development of the oxidative stress response induced by cold treatment. Addition of reduced compounds cystine and gluthathione suppressed the cold sensitive phenotype of the phaC mutant. Cold shock produced very rapid degradation of PHA in the wild type strain. The NADH/NAD ratio and NADPH content, estimated by diamide sensitivity, decreased strongly in the mutant after cold shock while only minor changes were observed in the wild type. Accordingly, the level of lipid peroxidation in the mutant strain was 25-fold higher after temperature downshift. We propose that PHA metabolism modulates the availability of reducing equivalents, contributing to alleviate the oxidative stress produced by low temperature. © 2008 Springer.

Registro:

Documento: Artículo
Título:Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation
Autor:Ayub, N.D.; Tribelli, P.M.; López, N.I.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, C1428EGA Buenos Aires, Argentina
Palabras clave:Antarctica; Cold exposure; Oxidative stress; Polyhydroxyalkanoates; Pseudomonas; Redox state; polyhydroxyalkanoic acid; primer DNA; adaptation; Antarctica; article; cold; genetics; growth, development and aging; mutation; nucleotide sequence; oxidation reduction reaction; physiology; Pseudomonas; Adaptation, Physiological; Antarctic Regions; Base Sequence; Cold Temperature; DNA Primers; Mutation; Oxidation-Reduction; Polyhydroxyalkanoates; Pseudomonas; Bacteria (microorganisms); Equus asinus; Pseudomonas; Pseudomonas sp. 14-3
Año:2009
Volumen:13
Número:1
Página de inicio:59
Página de fin:66
DOI: http://dx.doi.org/10.1007/s00792-008-0197-z
Título revista:Extremophiles
Título revista abreviado:Extremophiles
ISSN:14310651
CODEN:EXTRF
CAS:DNA Primers; Polyhydroxyalkanoates
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14310651_v13_n1_p59_Ayub

Referencias:

  • Ayub, N.D., Pettinari, M.J., Ruiz, J.A., López, N.I., A polyhydroxybutyrate-producing Pseudomonas sp. isolated from Antarctic environments with high stress resistance (2004) Curr Microbiol, 49, pp. 170-174
  • Ayub, N.D., Pettinari, M.J., Méndez, B.S., López, N.I., Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene (2006) FEMS Microbiol Lett, 264, pp. 125-131
  • Ayub, N.D., Pettinari, M.J., Méndez, B.S., López, N.I., The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island (2007) Plasmid, 58, pp. 240-248
  • Braunegg, G., Sonnleitner, B., Lafferty, R.M., A rapid gas chromatographic method for the determination of poly-ß-hydroxybutyric acid in microbial biomass (1978) Eur J Appl Microbial Biotechnol, 6, pp. 29-37
  • Butler, M.J., Bruheim, P., Jovetic, S., Marinelli, F., Postma, P.W., Bibb, M.J., Engineering of primary carbon metabolism for improved antibiotic production in Streptomyces lividans (2002) Appl Environ Microbiol, 68, pp. 4731-4739
  • D'Amico, S., Collins, T., Marx, J.C., Feller, G., Gerday, C., Psychrophilic microorganisms: Challenges for life (2006) EMBO Rep, 7, pp. 385-389
  • Franklin, F.C.H., Bagdasarian, M., Bagdasarian, M.M., Timmis, K.N., Molecular and functional analysis of the TOL plasmid pWW0 from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta-cleavage pathway (1981) Proc Natl Acad Sci USA, 78, pp. 7458-7462
  • Gocheva, Y.G., Krumova, E.T., Slokoska, L.S., Miteva, J.G., Vassilev, S.V., Angelova, M.B., Cell response of Antarctic and temperate strains of Penicillium spp. to different growth temperature (2006) Mycol Res, 110, pp. 1347-1354
  • Hirsch, P., Gallikowski, C.A., Siebert, J., Peissl, K., Kroppenstedt, R., Schumann, P., Stackebrandt, E., Anderson, R., Deinococcus frigens sp. nov., Deinococcus saxicola sp.nov., and Deinococcus marmoris sp.nov., low temperature and draught tolerating, UV-resistant bacteria from continental Antarctica (2004) Syst Appl Microbiol, 27, pp. 636-645
  • Huisman, G.W., Wonink, E., Meima, R., Kazemier, B., Terpstra, P., Witholt, B., Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. Identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA (1991) J Biol Chem, 266, pp. 2191-2198
  • Huisman, G.W., Wonink, E., De Koning, G., Preusting, H., Witholt, B., Synthesis of poly(3-hydroxyalkanoates) by mutant and recombinant Pseudomonas strains (1992) Appl Microbiol Biotechnol, 38, pp. 1-5
  • Kadouri, D., Jurkevitch, D., Okon, Y., Poly β-hydroxybutyrate depolymerase (PhaZ) in Azospirillum brasilense and characterization of a phaZ mutant (2003) Arch Microbiol, 180, pp. 309-318
  • Kadouri, D., Jurkevitch, E., Okon, Y., Castro-Sowinski, S., Ecological and agricultural significance of bacterial polyhydroxyalkanoates (2005) Crit Rev Microbiol, 31, pp. 55-67
  • Kane, D.O., Gill, V., Boyd, P., Burdon, R., Chilling, oxidative stress and antioxidant responses in Arabidopsis thaliana callus (1996) Planta, 198, pp. 371-377
  • Kato, C., Li, L., Nogi, Y., Nakamura, Y., Tamaoka, J., Horikoshi, K., Extremelly barophilic bacteria isolated from the Mariana Trench, Challenger Deep, at a depth of 11, 000 meters (1998) Appl Environ Microbiol, 64, pp. 1510-1513
  • Koonin, E.V., Makarova, K.S., Aravind, L., Horizontal gene transfer in Prokaryotes: Quantification and classification (2001) Annu Rev Microbiol, 55, pp. 709-742
  • Kovach, M.E., Elzer, P.H., Hill, D.S., Robertson, G.T., Farris, M.A., Roop, R.M., Peterson, K.M., Four new derivatives of the broad host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes (1995) Gene, 166, pp. 175-176
  • Lawrence, J.G., Gene transfer, speciation, and the evolution of bacterial genomes (1999) Curr Opin Microbiol, 2, pp. 519-523
  • Lee, S.H., Lee, S.Y., Park, B.C., Cell surface display of lipase in Pseudomonas putida KT2442 using OprF as an anchoring motif and its biocatalytic applications (2005) Appl Environ Microbiol, 71, pp. 8581-8586
  • Leonardo, M.R., Dailly, Y., Clark, D.P., Role of NAD in regulating the adhE gene of Escherichia coli (1996) J Bacteriol, 178, pp. 6013-6018
  • Liu, S., Graham, J.E., Bigelow, L., Morse, P.D., Wilkinson, B.J., Identification of Listeria monocytogenes genes expressed in response to growth at low temperature (2002) Appl Environ Microbiol, 68, pp. 1697-1705
  • López, N.I., Floccari, M.E., Garcia, A.F., Steinbüchel, A., Mendez, B.S., Effect of poly-3-hydroxybutyrate content on the starvation survival of bacteria in natural waters (1995) FEMS Microbiol Ecol, 16, pp. 95-101
  • Madison, L.L., Huisman, G.W., Metabolic engineering of poly (3-hydroxyalkanoates): From DNA to plastic (1999) Microbiol Mol Biol Rev, 63, pp. 21-53
  • Margesin, R., Neuner, G., Storey, K.B., Cold-loving microbes, plants, and animals-fundamental and applied aspects (2007) Naturwissenschaften, 94, pp. 77-99
  • Nelson, K.E., Weinel, C., Paulsen, I.T., Dodson, R.J., Hilbert, H., Martins Dos Santos, V.A., Fouts, D.E., Fraser, C.M., Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440 (2002) Environ Microbiol, 4, pp. 799-808
  • Ostle, A., Holt, J.G., Nile Blue a as a fluorescent stain for poly-hydroxybutyrate (1982) Appl Environ Microbiol, 44, pp. 238-241
  • Pham, T.H., Webb, J.S., Rehm, B.H.A., The role of polyhydroxyalkanoate biosynthesis by Pseudomonas aeruginosa in rhamnolipid and alginate production as well as stress tolerance and biofilm formation (2004) Microbiology, 150, pp. 3405-3413
  • Reva, O.N., Weinel, C., Weinel, M., Böhm, K., Stjepandic, D., Hoheisel, J.D., Tümmler, B., Functional genomics of stress response in Pseudomonas putida KT2440 (2006) J Bacteriol, 188, pp. 4079-4092
  • Ruiz, J.A., López, N.I., Fernández, R.O., Méndez, B.S., Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural waters microcosms (2001) Appl Environ Microbiol, 67, pp. 225-230
  • Ruiz, J.A., López, N.I., Méndez, B.S., RpoS gene expression in carbon starved cultures of the polyhydroxyalkanoate accumulating species Pseudomonas oleovorans (2004) Curr Microbiol, 48, pp. 396-400
  • Salahudeen, A.K., Huang, H., Patel, P., Jenkins, J.K., Mechanism and prevention of cold storage-induced human renal tubular cell injury (2000) Transplantation, 70, pp. 1424-1431
  • Semchyshyn, H., Bagnyukova, T., Storey, K., Lushchak, V., Hydrogen peroxide increases the activities of soxRS regulon enzymes and the levels of oxidized proteins and lipids in Escherichia coli (2005) Cell Biol Int, 29, pp. 898-902
  • Smirnova, G.V., Zakirova, O.N., Oktyabrskii, O.N., The role of antioxidant systems in the cold stress response of Escherichia coli (2001) Microbiology, 70, pp. 45-50
  • Yan, Y.B., Wu, Q., Zhang, R.Q., Dynamic accumulation and degradation of poly(3-hydroxyalkanoates) in living cells of Azotobacter vinelandii UWD characterized by 13C NMR (2000) FEMS Microbiol Lett, 193, pp. 269-273
  • Zhang, L., Onda, K., Imai, R., Fukuda, R., Horiuchi, H., Ohtaa, A., Growth temperature downshift induces antioxidant response in Saccharomyces cerevisiae (2003) Biochem Biophys Res Commun, 307, pp. 308-314

Citas:

---------- APA ----------
Ayub, N.D., Tribelli, P.M. & López, N.I. (2009) . Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation. Extremophiles, 13(1), 59-66.
http://dx.doi.org/10.1007/s00792-008-0197-z
---------- CHICAGO ----------
Ayub, N.D., Tribelli, P.M., López, N.I. "Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation" . Extremophiles 13, no. 1 (2009) : 59-66.
http://dx.doi.org/10.1007/s00792-008-0197-z
---------- MLA ----------
Ayub, N.D., Tribelli, P.M., López, N.I. "Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation" . Extremophiles, vol. 13, no. 1, 2009, pp. 59-66.
http://dx.doi.org/10.1007/s00792-008-0197-z
---------- VANCOUVER ----------
Ayub, N.D., Tribelli, P.M., López, N.I. Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation. Extremophiles. 2009;13(1):59-66.
http://dx.doi.org/10.1007/s00792-008-0197-z