Artículo

Bressa, M.J.; Papeschi, A.G.; Vítková, M.; Kubíková, S.; Fuková, I.; Pigozzi, M.I.; Marec, F. "Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)" (2009) Cytogenetic and Genome Research. 125(4):292-305
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Abstract:

The neo-X and neo-Y sex chromosomes of Dysdercus albofasciatus represent a unique model for the study of early stages of sex chromosome evolution since they retained the ability to pair and recombine, in contrast to sex chromosomes in most Heteroptera. Here we examined structure, molecular differentiation, and meiotic behaviour of the D. albofasciatus neo-sex chromosomes. Two related species with the ancestral X0 system, D. chaquensis and D. ruficollis, were used for a comparison. In D. albofasciatus, 2 nucleolar organizer regions (NORs) were identified on the neo-X chromosome using fluorescence in situ hybridization (FISH) with an rDNA probe, whereas a single NOR was found on an autosomal pair in the other 2 species. Genomic in situ hybridization (GISH) differentiated a part of the original X in the neo-X chromosome but not the neo-Y chromosome. The same segment of the neo-X chromosome was identified by Zoo-FISH with a chromosome painting probe derived from the X chromosome of D. ruficollis, indicating that this part is conserved between the species. Immunostaining against the cohesin subunit SMC3 revealed that only terminal regions of the D. albofasciatus neo-Xneo-Y bivalent pair and form a synaptonemal complex, which is in keeping with the occurrence of terminal chiasmata, whereas the interstitial region forms a large loop indicating the absence of homology. These results support the hypothesis that the neo-X chromosome evolved by insertion of the original X chromosome into 1 NOR-bearing autosome in an ancestor carrying the X0 system. As a consequence, the homologue of this NOR-autosome became the neo-Y chromosome. A subsequent inversion followed by transposition of the NOR located on the neo-Y onto the neo-X chromosome resulted in the present neo-sex chromosome system in D. albofasciatus. Copyright © 2009 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)
Autor:Bressa, M.J.; Papeschi, A.G.; Vítková, M.; Kubíková, S.; Fuková, I.; Pigozzi, M.I.; Marec, F.
Filiación:Laboratory of Cytogenetics and Evolution, Faculty of Exact and Natural Sciences, University of Buenos Aires, Buenos Aires, Argentina
Faculty of Science, University of South Bohemia, Czech Republic
Institute of Entomology, Biology Centre ASCR, České Budjovice, Czech Republic
Veterinary Research Institute, Brno, Czech Republic
Centre for Ecology and Conservation, University of Exeter, Cornwall Campus, Penryn, United Kingdom
Institute for Research in Reproduction, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina
Laboratorio de Citogenética y Evolución, Depto. de Ecologia, Genet. y Evol., Intendente Guiraldes y Costanera N. Cd. Univ. C1428EHA, Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Chromosome painting; Evolution; GISH; NOR; Ribosomal DNA; Sex chromosomes; Synaptonemal complex; Zoo-FISH; cohesin; ribosome DNA; 4',6 diamidino 2 phenylindole; fluorescent dye; indole derivative; animal experiment; animal tissue; article; autosome; chromosome band; chromosome C band; chromosome NOR; chromosome painting; chromosome pairing; controlled study; Dysdercus albofasciatus; Dysdercus chaquensis; Dysdercus ruficollis; fluorescence in situ hybridization; genomic in situ hybridization; Heteroptera; immunohistochemistry; karyotype; karyotype evolution; male; nonhuman; priority journal; sex chromosome; species difference; synaptonemal complex; X chromosome; Y chromosome; animal; biotinylation; chemistry; chromosome; chromosome banding pattern; DNA probe; female; genetics; karyotyping; meiosis; metabolism; metaphase; molecular evolution; X chromosome; Y chromosome; Dysdercus; Dysdercus ruficollis; Heteroptera; Pyrrhocoridae; Wupatkius semo; Animals; Biotinylation; Chromosome Banding; Chromosome Painting; Chromosomes; DNA Probes; DNA, Ribosomal; Evolution, Molecular; Female; Fluorescent Dyes; Heteroptera; Immunohistochemistry; In Situ Hybridization, Fluorescence; Indoles; Karyotyping; Male; Meiosis; Metaphase; Nucleolus Organizer Region; Species Specificity; Synaptonemal Complex; X Chromosome; Y Chromosome
Año:2009
Volumen:125
Número:4
Página de inicio:292
Página de fin:305
DOI: http://dx.doi.org/10.1159/000235936
Título revista:Cytogenetic and Genome Research
Título revista abreviado:Cytogenet. Genome Res.
ISSN:14248581
CODEN:CGRYA
CAS:4',6 diamidino 2 phenylindole, 47165-04-8; DAPI, 47165-04-8; DNA Probes; DNA, Ribosomal; Fluorescent Dyes; Indoles
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248581_v125_n4_p292_Bressa

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Citas:

---------- APA ----------
Bressa, M.J., Papeschi, A.G., Vítková, M., Kubíková, S., Fuková, I., Pigozzi, M.I. & Marec, F. (2009) . Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae). Cytogenetic and Genome Research, 125(4), 292-305.
http://dx.doi.org/10.1159/000235936
---------- CHICAGO ----------
Bressa, M.J., Papeschi, A.G., Vítková, M., Kubíková, S., Fuková, I., Pigozzi, M.I., et al. "Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)" . Cytogenetic and Genome Research 125, no. 4 (2009) : 292-305.
http://dx.doi.org/10.1159/000235936
---------- MLA ----------
Bressa, M.J., Papeschi, A.G., Vítková, M., Kubíková, S., Fuková, I., Pigozzi, M.I., et al. "Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)" . Cytogenetic and Genome Research, vol. 125, no. 4, 2009, pp. 292-305.
http://dx.doi.org/10.1159/000235936
---------- VANCOUVER ----------
Bressa, M.J., Papeschi, A.G., Vítková, M., Kubíková, S., Fuková, I., Pigozzi, M.I., et al. Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae). Cytogenet. Genome Res. 2009;125(4):292-305.
http://dx.doi.org/10.1159/000235936