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Abstract:

Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose at different concentrations and temperatures. When glucose concentrations of 15 and 1.5 mM were assayed, calculated activation energies were 25.2 and 18.4 kcal mol-1, respectively, in good agreement with previously published data. The opportunity and convenience of using Arrhenius plots to estimate the activation energy in metabolic-related processes is also discussed. © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

Registro:

Documento: Artículo
Título:Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach
Autor:Kormes, D.J.; Cortón, E.
Filiación:Biosensors and Bioanalysis Group, FCEN, UBA / Department of Biochemistry, Universidad de Buenos Aires, Ciudad Universitaria, Pab. 2, (1428) Buenos Aires, Argentina
Biosensors and Bioanalysis Group, FCEN, UBA and CONICET / Department of Biochemistry, Universidad de Buenos Aires, Ciudad Universitaria, Pab. 2, (1428) Buenos Aires, Argentina
Palabras clave:Activation energy; Arrhenius plot; Biosensors; Glucose transport; Microbial metabolism; Saccharomyces cerevisiae; Analytical tool; Cellular membranes; Glucose concentration; Glucose transport; In-vivo; Microbial cells; Microbial metabolism; S.cerevisiae; Activation energy; Arrhenius plots; Cell membranes; Glucose; Metabolism; Yeast; Biosensors
Año:2009
Volumen:9
Número:3
Página de inicio:1599
Página de fin:1608
DOI: http://dx.doi.org/10.3390/s90301599
Título revista:Sensors
Título revista abreviado:Sensors
ISSN:14248220
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_14248220_v9_n3_p1599_Kormes.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248220_v9_n3_p1599_Kormes

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Citas:

---------- APA ----------
Kormes, D.J. & Cortón, E. (2009) . Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach. Sensors, 9(3), 1599-1608.
http://dx.doi.org/10.3390/s90301599
---------- CHICAGO ----------
Kormes, D.J., Cortón, E. "Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach" . Sensors 9, no. 3 (2009) : 1599-1608.
http://dx.doi.org/10.3390/s90301599
---------- MLA ----------
Kormes, D.J., Cortón, E. "Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach" . Sensors, vol. 9, no. 3, 2009, pp. 1599-1608.
http://dx.doi.org/10.3390/s90301599
---------- VANCOUVER ----------
Kormes, D.J., Cortón, E. Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach. Sensors. 2009;9(3):1599-1608.
http://dx.doi.org/10.3390/s90301599