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Abstract:

Nitrogen compounds like urea and melamine are known to be commonly used for milk adulteration resulting in undesired intoxication; a well-known example is the Chinese episode occurred in 2008. The development of a rapid, reliable and economic test is of relevance in order to improve adulterated milk identification. Cyclic voltammetry studies using an Au working electrode were performed on adulterated and non-adulterated milk samples from different independent manufacturers. Voltammetric data and their first derivative were subjected to functional principal component analysis (f-PCA) and correctly classified by the KNN classifier. The adulterated and non-adulterated milk samples showed significant differences. Best results of prediction were obtained with first derivative data. Detection limits in milk samples adulterated with 1% of its total nitrogen derived from melamine or urea were as low as 85.0 mg·L-1 and 121.4 mg·L-1, respectively. We present this method as a fast and robust screening method for milk adulteration analysis and prevention of food intoxication. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

Registro:

Documento: Artículo
Título:Voltamperometric discrimination of urea and melamine adulterated skimmed milk powder
Autor:Hilding-Ohlsson, A.; Fauerbach, J.A.; Sacco, N.J.; Bonetto, M.C.; Cortón, E.
Filiación:Biosensors and Bioanalysis Lab, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (IQUIBICEN, CONICET), CABA 1428, Argentina
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (CIHIDECAR, CONICET), CABA 1428, Argentina
Palabras clave:F-PCA; KNN; Milk adulteration; Rapid screening methods; Voltammetry; F-PCA; Functional principal component analysis; KNN; Milk adulterations; Rapid screening; Screening methods; Significant differences; Voltammetric data; Cyclic voltammetry; Metabolism; Principal component analysis; Voltammetry; Urea; melamine; triazine derivative; urea; animal; article; chemistry; dairy product; f-PCA; food contamination; KNN; methodology; milk; milk adulteration; potentiometry; powder; principal component analysis; rapid screening methods; f-PCA; KNN; milk adulteration; rapid screening methods; voltammetry; Animals; Dairy Products; Food Contamination; Milk; Powders; Principal Component Analysis; Triazines; Urea
Año:2012
Volumen:12
Número:9
Página de inicio:12220
Página de fin:12234
DOI: http://dx.doi.org/10.3390/s120912220
Título revista:Sensors (Switzerland)
Título revista abreviado:Sensors
ISSN:14248220
CAS:melamine, 108-78-1, 25778-04-5; urea, 57-13-6; Powders; Triazines; Urea, 8W8T17847W; melamine, N3GP2YSD88
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_14248220_v12_n9_p12220_HildingOhlsson.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248220_v12_n9_p12220_HildingOhlsson

Referencias:

  • Souza, S.S., Cruz, A.G., Walter, E.H.M., Faria, J.A.F., Celeghini, R.M.S., Ferreira, M.M.C., Granato, D., de Sant'Ana, A., Monitoring the authenticity of Brazilian UHT milk: A chemometric approach (2011) Food Chem, 124, pp. 692-695
  • Paradkar, M.M., Singhal, R.S., Kulkarni, P.R., An approach to the detection of synthetic milk in dairy milk: 1. Detection of urea (2000) Int. J. Dairy Technol, 53, pp. 87-91
  • Trivedi, U.B., Lakhminarayana, D., Kothari, L., Patel, G., Kapse, N., Makhija, K., Patel, B., Panchal, J., Potentiometric biosensor for urea determination in milk (2009) Sens. Actuators B: Chem, 140, pp. 260-266
  • Xia, J., Zhou, N., Liu, Y., Chen, B., Wu, Y., Yao, S., Simultaneous determination of melamine and related compounds by capillary zone electrophoresis (2010) Food Control, 21, pp. 912-918
  • Moore, J.C., de Vries, J.W., Lipp, M., Griffiths, J.C., Abernethy, D.R., Total protein methods and their potential utility to reduce the risk of food protein adulteration (2010) Compr. Rev. Food Sci. Food Saf, 9, pp. 330-357
  • Jonker, J.S., Kohn, R.A., Erdman, R.A., Using milk urea nitrogen to predict nitrogen excretion and utilization efficiency in lactating dairy cows (1998) J. Dairy Sci, 81, pp. 2681-2692
  • Sharma, R., Rajput, Y.S., Kaur, S., Tomar, S.K., A method for estimation of urea using ammonia electrode and its applicability to milk samples (2008) J. Dairy Res, 58, pp. 466-477
  • Nikoleli, G.-N., Nikolelis, D.P., Methenitis, C., Construction of a simple optical sensor based on air stable lipid film with incorporated urease for the rapid detection of urea in milk (2010) Anal. Chim. Acta, 675, pp. 58-63
  • Liang, R., Zhang, R., Qin, W., Potentiometric sensor based on molecularly imprinted polymer for determination of melamine in milk (2009) Sens. Actuators B: Chem, 141, pp. 544-550
  • Zhang, L., Wu, L.-L., Wang, Y.-P., Liu, A.-M., Zou, C.-C., Zhao, Z.-Y., Melamine-Contaminated milk products induced urinary tract calculi in children (2009) World J. Pediatr, 5, pp. 31-35
  • Jia, X.-D., Li, N., Wang, Z.-T., Zhao, Y.-F., Wu, Y.-N., Yan, W.-X., Assessment on dietary melamine exposure from tainted infant formula (2009) Biomed. Environ. Sci, 22, pp. 100-103
  • Xin, H., Stone, R., Tainted milk scandal. Chinese probe unmasks high-tech adulteration with melamine (2008) Science, 322, pp. 1310-1311
  • (2008) Expert Meeting In Collaboration With FAO. Toxicological and Health Aspects of Melamine and Cyanuric Acid, , World Health Organization (WHO). Health Canada: Ottawa, ON, Canada, 1-4 December
  • Desmarchelier, A., Guillamon Cuadra, M., Delatour, T., Mottier, P., Simultaneous quantitative determination of melamine and cyanuric acid in cow's milk and milk-based infant formula by liquid chromatography-electrospray ionization tandem mass spectrometry (2009) J. Agric. Food Chem, 57, pp. 7186-7193
  • Mamat, M., Samad, S.A., Hannan, M.A., An electronic nose for reliable measurement and correct classification of beverages (2011) Sensors, 11, pp. 6435-6453
  • Paixao, T.R.L.C., Bertotti, M., Fabrication of disposable voltammetric electronic tongues by using prussian blue films electrodeposited onto CD-R gold surfaces and recognition of milk adulteration (2009) Sens. Actuators B: Chem, 137, pp. 266-273
  • Vlasov, Y., Legin, A., Rudnitskaya, A., Di Natale, C., D'amico, A., Nonspecific sensor arrays ("electronic tongue") for chemical analysis of liquids. (IUPAC Technical Report) (2005) Pure Appl. Chem, 77, pp. 1965-1983
  • Escuder-Gilabert, L., Peris, M., Review: Highlights in recent applications of electronic tongues in food analysis (2010) Anal. Chim. Acta, 665, pp. 15-25
  • Tian, X.-Y., Cai, Q., Zhang, Y.-M., Rapid classification of hairtail fish and pork freshness using an electronic nose based on the PCA method (2012) Sensors, 12, pp. 260-277
  • Dias, L.A., Peres, A.M., Veloso, A.C.A., Reis, F.S., Vilas-Boas, M., Machado, A.A.S.C., An electronic tongue taste evaluation: Identification of goat milk adulteration with bovine milk (2009) Sens. Actuators B: Chem, 136, pp. 209-217
  • Ciosek, P., Brzózka, Z., Wróblewski, W., Martinelli, E., Di Natale, C., D'amico, A., Direct and two-stage data analysis procedures based on PCA, PLS-DA and ANN for ISE-based electronic tongue -Effect of supervised feature extraction (2005) Talanta, 67, pp. 590-596
  • Manea, F., Pop, A., Radovan, C., Malchev, P., Bebeselea, A., Burtica, G., Picken, S., Schoonman, J., Voltammetric detection of urea on an Ag-modified zeolite-expanded graphite-epoxy composite (2008) Sensors, 8, pp. 5806-5819
  • Winquist, F., Krantz-Rülcker, C., Wide, P., Lundström, I., Monitoring of freshness of milk by an electronic tongue on the basis of voltammetry (1998) Meas. Sci. Technol, 9, pp. 1937-1946
  • Winquist, F., Wide, P., Lundström, I., An electronic tongue based on voltammetry (1997) Anal. Chim. Acta, 357, pp. 21-31
  • Winquist, F., Holmin, S., Krantz-Rülcker, C., Wide, P., Lundström, I., An hybrid electronic tongue (2000) Anal. Chim. Acta, 406, pp. 147-157
  • Ramsay, J.O., Silverman, B.W., (2005) Functional Data Analysis. Springer Series In Statistics, p. 428. , 2nd ed.; Springer: New York, NY, USA
  • Ramsay, J.O., Hooker, G., Graves, S., (2009) Functional Data Analysis With R and MATLAB, p. 202. , Springer: New York, NY, USA
  • Gutierrez, M., Llobera, A., Ipatov, A., Vila-Planas, J., Mínguez, S., Demming, S., Büttgenbach, S., Jiménez-Jorquera, C., Application of an e-tongue to the analysis of monovarietal and blends of white wines (2011) Sensors, 11, pp. 4840-4857
  • Heras, J.Y., Rodriguez, S.D., Negri, R.M., Battaglini, F., Chelating electrodes as taste sensor for the trace assessment of metal ions (2010) Sens. Actuators B: Chem, 145, pp. 726-733
  • Pigani, L., Foca, G., Ulrici, A., Ionescu, K., Martina, V., Terzi, F., Vignali, M., Seeber, R., Classification of red wines by chemometric analysis of voltammetric signals from PEDOT-modified electrodes (2009) Anal. Chim. Acta, 643, pp. 67-73
  • Schreyer, S.K., Mikkelsen, S.R., Chemometric analysis of square wave voltammograms for classification and quantitation of untreated beverage samples (2000) Sens. Actuators B: Chem, 71, pp. 147-153
  • Karoui, R., de Baerdemaeker, J., A review of the analytical methods coupled with chemometric tools for the determination of the quality and identity of dairy products (2007) Food Chem, 102, pp. 621-640
  • Sola-Larrañaga, C., Navarro-Blasco, I., (2009) Food Chem, 112, pp. 189-196. , Chemometric analysis of minerals and trace elements in raw cow milk from the community of Navarra, Spain
  • (2009) R: A Language and Environment For Statistical Computing, p. 409. , R Development Core Team, R Foundation for Statistical Computing: Vienna, Austria
  • Chen, J., Gorton, L., Akerson, B., Electrochemical studies on antioxidants in bovine milk (2002) Anal. Chim. Acta, 474, pp. 137-146
  • Jawad, M.A., Dorie, J., El Murr, N., Electrochemical quantitative analysis of uric acid in milk (1991) J. Food Sci, 56, pp. 594-595
  • Tsai, T.-H., Thiagarajan, S., Chen, S.-M., Detection of melamine in milk powder and human urine (2010) J. Agric. Food Chem, 58, pp. 4537-4544
  • Holze, R., Shomaker, S., New results on the electrosorption of urea and thiourea on gold electrodes (1990) Electrochim. Acta, 35, pp. 613-620
  • Winquist, F., Bjorklund, R., Krantz-Ruckler, C., Lundström, I., Ostergren, K., Skoglund, T., An electronic tongue in the dairy industry (2005) Sens. Actuators B: Chem, pp. 111-112+299-304
  • Liu, Y.T., Deng, J., Xiao, X.L., Ding, L., Yuan, Y.L., Li, H., Li, X.T., Wang, L.L., Electrochemical sensor based on poly(para-aminobenzoic acid) film modified glassy carbon electrode for the determination of melamine in milk (2011) Electrochim. Acta, 56, pp. 4595-4602
  • Tittlemier, S.A., Methods for the analysis of melamine and related compounds in foods: A review (2010) Food Addit. Contam, 27, pp. 129-145

Citas:

---------- APA ----------
Hilding-Ohlsson, A., Fauerbach, J.A., Sacco, N.J., Bonetto, M.C. & Cortón, E. (2012) . Voltamperometric discrimination of urea and melamine adulterated skimmed milk powder. Sensors (Switzerland), 12(9), 12220-12234.
http://dx.doi.org/10.3390/s120912220
---------- CHICAGO ----------
Hilding-Ohlsson, A., Fauerbach, J.A., Sacco, N.J., Bonetto, M.C., Cortón, E. "Voltamperometric discrimination of urea and melamine adulterated skimmed milk powder" . Sensors (Switzerland) 12, no. 9 (2012) : 12220-12234.
http://dx.doi.org/10.3390/s120912220
---------- MLA ----------
Hilding-Ohlsson, A., Fauerbach, J.A., Sacco, N.J., Bonetto, M.C., Cortón, E. "Voltamperometric discrimination of urea and melamine adulterated skimmed milk powder" . Sensors (Switzerland), vol. 12, no. 9, 2012, pp. 12220-12234.
http://dx.doi.org/10.3390/s120912220
---------- VANCOUVER ----------
Hilding-Ohlsson, A., Fauerbach, J.A., Sacco, N.J., Bonetto, M.C., Cortón, E. Voltamperometric discrimination of urea and melamine adulterated skimmed milk powder. Sensors. 2012;12(9):12220-12234.
http://dx.doi.org/10.3390/s120912220