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Abstract:

A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

Registro:

Documento: Artículo
Título:Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
Autor:Ferro, Y.; Perullini, M.; Jobbagy, M.; Bilmes, S.A.; Durrieu, C.
Filiación:Université de Lyon, ENTPE, CNRS, UMR5023 LEHNA, Rue Maurice Audin, 69518 Vaulx-en-Velin, France
INQUIMAE-DQIAQF, Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria Pab. II, C1428EHA Buenos Aires, Argentina
Palabras clave:Algae; Atrazine; Biosensor; Chlorophyll fluorescence; DCMU; Herbicides; Sol-gel; Aquatic environments; Chlamydomonas reinhardtii; Chlorophyll fluorescence; DCMU; Environmental Monitoring; Fluorescence enhancement; Pseudokirchneriella subcapitata; Two-step procedure; Algae; Herbicides; Hydrogels; Microorganisms; Silica; Sol-gels; Weed control; Biosensors; atrazine; diuron; fresh water; article; chemistry; Chlamydomonas reinhardtii; Chlorella vulgaris; environmental monitoring; equipment; genetic procedures; human; hydrogel; isolation and purification; microalga; water pollutant; Atrazine; Biosensing Techniques; Chlamydomonas reinhardtii; Chlorella vulgaris; Diuron; Environmental Monitoring; Fresh Water; Humans; Hydrogel; Microalgae; Water Pollutants, Chemical
Año:2012
Volumen:12
Número:12
Página de inicio:16879
Página de fin:16891
DOI: http://dx.doi.org/10.3390/s121216879
Título revista:Sensors (Switzerland)
Título revista abreviado:Sensors
ISSN:14248220
CAS:atrazine, 1912-24-9; diuron, 330-54-1; Atrazine, QJA9M5H4IM; Diuron, 9I3SDS92WY; Hydrogel, 25852-47-5; Water Pollutants, Chemical
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_14248220_v12_n12_p16879_Ferro.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248220_v12_n12_p16879_Ferro

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Citas:

---------- APA ----------
Ferro, Y., Perullini, M., Jobbagy, M., Bilmes, S.A. & Durrieu, C. (2012) . Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels. Sensors (Switzerland), 12(12), 16879-16891.
http://dx.doi.org/10.3390/s121216879
---------- CHICAGO ----------
Ferro, Y., Perullini, M., Jobbagy, M., Bilmes, S.A., Durrieu, C. "Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels" . Sensors (Switzerland) 12, no. 12 (2012) : 16879-16891.
http://dx.doi.org/10.3390/s121216879
---------- MLA ----------
Ferro, Y., Perullini, M., Jobbagy, M., Bilmes, S.A., Durrieu, C. "Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels" . Sensors (Switzerland), vol. 12, no. 12, 2012, pp. 16879-16891.
http://dx.doi.org/10.3390/s121216879
---------- VANCOUVER ----------
Ferro, Y., Perullini, M., Jobbagy, M., Bilmes, S.A., Durrieu, C. Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels. Sensors. 2012;12(12):16879-16891.
http://dx.doi.org/10.3390/s121216879