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Abstract:

We describe the preparation and photophysical characterization of two model compounds designed to test a new approach for the quantitative determination of Fluorescence Resonance Energy Transfer (FRET) in biological systems. The method enables modulation of FRET by exploiting the unique reversible spectral properties of photochromic diarylethenes and spiropyrans to create switchable energy acceptors.

Registro:

Documento: Artículo
Título:Fluorescence resonance energy transfer using spiropyran and diarylethene photochromic acceptors
Autor:Giordano, L.; Macareno, J.; Song, L.; Jovin, T.M.; Irie, M.; Jares-Erijman, E.A.
Filiación:Departamento de Quimica Organica, PROPLAME-CONICET, UBA, Argentina
Department of Molecular Biology, MPI for Biophys. Chem., Göttingen, Germany
Dept. of Chemistry and Biochemistry, Kyushu University, Fukuoka, Japan
Palabras clave:ethylene; absorption spectroscopy; analytic method; conference paper; energy transfer; fluorescence; quantitative analysis; Raman spectrometry; structure analysis
Año:2000
Volumen:5
Número:3
Página de inicio:591
Página de fin:593
DOI: http://dx.doi.org/10.3390/50300591
Título revista:Molecules
Título revista abreviado:Molecules
ISSN:14203049
CODEN:MOLEF
CAS:ethylene, 74-85-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14203049_v5_n3_p591_Giordano

Referencias:

  • Förster, T., (1946) Naturwissenschaften, 6, p. 166
  • Jares-Erijman, E.A., Song, L., Jovin, T.M., (1997) Mol. Crystals and Liquid Crystals, 298, p. 151
  • Song, L., Jares-Erijman, E., Jovin, T.M., (1999) Biophysical J., , submitted
  • Bastiaens, P.I.H., Majoul, I.V., Verveer, P.J., Soling, H.-D., Jovin, T.M., (1996) EMBO J., 15, p. 4246

Citas:

---------- APA ----------
Giordano, L., Macareno, J., Song, L., Jovin, T.M., Irie, M. & Jares-Erijman, E.A. (2000) . Fluorescence resonance energy transfer using spiropyran and diarylethene photochromic acceptors. Molecules, 5(3), 591-593.
http://dx.doi.org/10.3390/50300591
---------- CHICAGO ----------
Giordano, L., Macareno, J., Song, L., Jovin, T.M., Irie, M., Jares-Erijman, E.A. "Fluorescence resonance energy transfer using spiropyran and diarylethene photochromic acceptors" . Molecules 5, no. 3 (2000) : 591-593.
http://dx.doi.org/10.3390/50300591
---------- MLA ----------
Giordano, L., Macareno, J., Song, L., Jovin, T.M., Irie, M., Jares-Erijman, E.A. "Fluorescence resonance energy transfer using spiropyran and diarylethene photochromic acceptors" . Molecules, vol. 5, no. 3, 2000, pp. 591-593.
http://dx.doi.org/10.3390/50300591
---------- VANCOUVER ----------
Giordano, L., Macareno, J., Song, L., Jovin, T.M., Irie, M., Jares-Erijman, E.A. Fluorescence resonance energy transfer using spiropyran and diarylethene photochromic acceptors. Molecules. 2000;5(3):591-593.
http://dx.doi.org/10.3390/50300591