Abstract:
E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine.
Registro:
Documento: |
Artículo
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Título: | Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides |
Autor: | Rogert, M.C.; Martínez, N.; Porro, S.; Lewkowicz, E.; Iribarren, A. |
Filiación: | Universidad Nacional de Quilmes, R. S. Peña 180, (1876) Bernal, Buenos Aires, Argentina INGEBI, CONICET, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
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Palabras clave: | purine nucleoside derivative; bacterial strain; biocatalyst; conference paper; drug synthesis; Escherichia coli; kinetics; nonhuman; pH measurement; quantum yield; temperature dependence |
Año: | 2000
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Volumen: | 5
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Número: | 3
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Página de inicio: | 535
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Página de fin: | 536
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DOI: |
http://dx.doi.org/10.3390/50300535 |
Título revista: | Molecules
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Título revista abreviado: | Molecules
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ISSN: | 14203049
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CODEN: | MOLEF
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14203049_v5_n3_p535_Rogert |
Referencias:
- Pal, S., Nair, V., (1997) Biocatal. and Biotransf., 15, p. 147
- Utagawa, T., Morisawa, H., Yoshinaga, F., Yamazaki, A., Mitsugi, K., Hirose, Y., (1985) Agric. Biol. Chem., 49 (4), p. 1053
- Krenitsky, T., Koszalka, G., Tuttle, J., (1981) Biochemistry, 20, p. 3615
Citas:
---------- APA ----------
Rogert, M.C., Martínez, N., Porro, S., Lewkowicz, E. & Iribarren, A.
(2000)
. Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides. Molecules, 5(3), 535-536.
http://dx.doi.org/10.3390/50300535---------- CHICAGO ----------
Rogert, M.C., Martínez, N., Porro, S., Lewkowicz, E., Iribarren, A.
"Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides"
. Molecules 5, no. 3
(2000) : 535-536.
http://dx.doi.org/10.3390/50300535---------- MLA ----------
Rogert, M.C., Martínez, N., Porro, S., Lewkowicz, E., Iribarren, A.
"Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides"
. Molecules, vol. 5, no. 3, 2000, pp. 535-536.
http://dx.doi.org/10.3390/50300535---------- VANCOUVER ----------
Rogert, M.C., Martínez, N., Porro, S., Lewkowicz, E., Iribarren, A. Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides. Molecules. 2000;5(3):535-536.
http://dx.doi.org/10.3390/50300535