Artículo

Este artículo es de Acceso Abierto y puede ser descargado en su versión final desde nuestro repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Pseudacteon flies, parasitoids of worker ants, are being intensively studied as potentially effective agents in the biological control of the invasive pest fire ant genus Solenopsis (Hymenoptera: Formicidae). This is the first attempt to describe the karyotype of P. curvatus Borgmeier, P. nocens Borgmeier and P. tricuspis Borgmeier. The three species possess 2n = 6; chromosomes I and II were metacentric in the three species, but chromosome pair III was subtelocentric in P. curvatus and P. tricuspis, and telocentric in P. nocens. All three species possess a C positive band in chromosome II, lack C positive heterochromatin on chromosome I, and are mostly differentiated with respect to chromosome III. P. curvatus and P. tricuspis possess a C positive band, but at different locations, whereas this band is absent in P. nocens. Heterochromatic bands are neither AT nor GC rich as revealed by fluorescent banding. In situ hybridization with an 18S rDNA probe revealed a signal on chromosome II in a similar location to the C positive band in the three species. The apparent lack of morphologically distinct sex chromosomes is consistent with proposals of environmental sex determination in the genus. Small differences detected in chromosome length and morphology suggests that chromosomes have been highly conserved during the evolutionary radiation of Pseudacteon. Possible mechanisms of karyotype evolution in the three species are suggested. Copyright © 2009, Sociedade Brasileira de Genética.

Registro:

Documento: Artículo
Título:Cytogenetic analysis of three species of Pseudacteon (Diptera, Phoridae) parasitoids of the fire ants using standard and molecular techniques
Autor:Chirino, M.G.; Folgarait, P.J.; Gilbert, L.E.; Lanzavecchia, S.; Papeschi, A.G.
Filiación:Centro de Estudios e Investigaciones, Universidad Nacional de Quilmes, Roque Saenz Peña 352, B1876BXD Bernal, Buenos Aires, Argentina
Brackenridge Field Laboratory, University of Texas, Austin, TX, United States
Instituto de Genética, INTA, Castelar, Buenos Aires, Argentina
Laboratorio de Citogenética Y Evolución, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:C-banding; FISH; Fluorescent banding; Karyotype evolution; Pseudacteon; DNA 18S; animal cell; animal experiment; ant; article; AT rich sequence; chromosome; chromosome analysis; chromosome C band; chromosome I; chromosome II; chromosome III; chromosome size; chromosome structure; DNA probe; fire ant; fluorescence; fly; GC rich sequence; heterochromatin; in situ hybridization; interphase; karyotype; metaphase chromosome; mitosis; nonhuman; parasitoid; Pseudacteon; Pseudacteon curvatus; Pseudacteon nocens; Pseudacteon tricuspis; sex chromosome; species difference; Borgmeier; Diptera; Formicidae; Hymenoptera; Phoridae; Pseudacteon (dipteran fly); Pseudacteon curvatus; Pseudacteon tricuspis; Solenopsis; Solenopsis geminata
Año:2009
Volumen:32
Número:4
Página de inicio:740
Página de fin:747
DOI: http://dx.doi.org/10.1590/S1415-47572009005000073
Título revista:Genetics and Molecular Biology
Título revista abreviado:Genet. Mol. Biol.
ISSN:14154757
CODEN:GMBIF
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_14154757_v32_n4_p740_Chirino.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14154757_v32_n4_p740_Chirino

Referencias:

  • Baruffi, L., Damiani, G., Guglielmino, C., Bandi, C., Malacrida, A., Gasperi, G., Polymorphism within and between population of Ceratitis capitata. Comparison between RAPD and Multilocus enzyme electrophoresis data (1995) Heredity, 74, pp. 524-537
  • Bedo, D.G., Webb, G.C., Conservation of nucleolar structure in polytene tissues of Ceratitis capitata (Diptera, Tephritidae) (1989) Chromosoma, 98, pp. 443-449
  • Brown, B.V., Folgarait, P.J., Gilbert, L., A new species of Pseudacteon attacking solenopsis fire ants (Hymenoptera, Formicidae) in Argentina (2003) Sociobiology, 41, pp. 685-688
  • Calcaterra, L.A., Pseudacteon calderensis, a new fly species (Diptera, Phoridae) attacking the fire ants Solenopsis interrupta (Hymenoptera, Formicidae) in northwestern Argentina (2007) Ann Entomol Soc Am, 100, pp. 470-473
  • Calcaterra, L.A., Delgado, A., Tsutsui, N.D., Activity patterns and parasitism rates of fire ant-decapitating flies (Diptera, Phoridae, Pseudacteon spp.) in their native Argentina (2008) Ann Entomol Soc Am, 101, pp. 539-550
  • Cevallos, V.E., Nation, J.L., Chromosomes of the Caribbean fruit fly (Diptera, Tephritidae) (2004) Fla Entomol, 87, pp. 361-364
  • Chirino, M.G., Gilbert, L.E., Folgarait, P.J., Behavior and development of Pseudacteon curvatus (Diptera, Phoridae) varies according to the social form of its host Solenopsis invicta (Hymenoptera, Formicidae) in its native range (2009) Environ Entomol, 38, pp. 198-206
  • Disney, R.H.L., (1994) Scuttle Flies: The Phoridae, , Chapman & Hall, London, 480 pp
  • Folgarait, P.J., Bruzzone, O.A., Gilbert, L.E., Development of Pseudacteon cultellatus (Diptera, Phoridae) on Solenopsis invicta and Solenopsis richteri fire ants (Hymenoptera, Formicidae) (2002) Environ Entomol, 31, pp. 403-410
  • Folgarait, P.J., Chirino, M.G., Wilson Patrock, R.J., Gilbert, L.E., Development of Pseudacteon obtusus (Diptera, Phoridae) on Solenopsis invicta and Solenopsis richteri fire ants (Hymenoptera, Formicidae) (2005) Environ Entomol, 34, pp. 308-316
  • Folgarait, P.J., Bruzzone, O.A., Porter, S.D., Pesquero, M.A., Gilbert, L.E., Biogeography and macroecology of phorid flies that attack fire ants in southeastern Brazil and Argentina (2005) J Biogeogr, 32, pp. 353-367
  • Folgarait, P.J., Wilson Patrock, R.J., Gilbert, L.E., Development of Pseudacteon nocens (Diptera, Phoridae) on Solenopsis invicta and Solenopsis richteri fire ants (Hymenoptera, Formicidae) (2006) J Econ Entomol, 99, pp. 295-307
  • Folgarait, P.J., Patrock, R.J.W., Gilbert, L.E., Associations of fire ant phorids and microhabitats (2007) Environ Entomol, 36, pp. 731-742
  • Folgarait, P.J., Patrock, R.J.W., Gilbert, L.E., The influence of ambient conditions and space on the phenological patterns of a Solenopsis phorid guild in an arid environment (2007) Biol Control, 42, pp. 262-273
  • Fuková, I., Nguyen, P., Marec, F., Codling moth cytogenetics: Karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes (2005) Genome, 48, pp. 1083-1092
  • Gilbert, F., Jervis, M., Functional, evolutionary and ecological aspects of feeding-related mouthpart specializations in parasitoid flies (1998) Biol J Linn Soc, 63, pp. 495-535
  • Howell, W.M., Black, A.D., Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: A 1-step method (1980) Experientia, 36, pp. 1014-1015
  • Kronforst, M.R., Folgarait, P.J., Patrock, R.J.W., Gilbert, L.E., Genetic differentiation between body size biotypes of the parasitoid fly Pseudacteon obtusus (Diptera, Phoridae) (2007) Mol Phylogenet Evol, 43, pp. 1178-1184
  • Levan, A., Fredga, K., Sandberg, A., Nomenclature for centromeric position on chromosomes (1964) Hereditas, 52, pp. 201-220
  • Mainx, F., The genetics of Megaselia scalaris Loew (Phoridae): A new type of sex determination in Diptera (1964) Am Nat, 98, pp. 415-430
  • Marchi, A., Pili, E., Ribosomal RNA genes in mosquitoes: Localization by fluorescence in situ hybridization (FISH) (1994) Heredity, 72, pp. 599-605
  • Mescher, M.C., Ross, K.G., Shoemaker, D.D., Keller, L., Krieger, M.J.B., Distribution of the two social forms of the fire ant Solenopsis invicta (Hymenoptera, Formicidae) in the native South American range (2003) Ann Entomol Soc Am, 96, pp. 810-817
  • Morrison, L.W., Porter, S.D., Post-release host-specificity testing of Pseudacteon tricuspis, a phorid parasitoid of Solenopsis invicta fire ants (2006) Biocontrol, 51, pp. 195-205
  • Morrison, L.W., Dall'aglio-Holvorcem, C.G., Gilbert, L., Oviposition behavior and development of Pseudacteon flies (Diptera, Phoridae), parasitoids of Solenopsis fire ants (Hymenoptera, Formicidae) (1997) Environ Entomol, 26, pp. 14-22
  • Morrison, L.W., Porter, S.D., Gilbert, L.E., Sex ratio variations as a function of host size in Pseudacteon flies (Diptera, Phoridae), parasitoids of Solenopsis fire ants (Hymenoptera, Formicidae) (1999) Biol J Linn Soc, 66, pp. 257-267
  • Motara, M.A., Pathak, S., Satya-Prakash, K.L., Hsu, T.C., Argentophilic structures of spermatogenesis in the yellow fever mosquito (1985) J Hered, 76, pp. 295-300
  • Parise-Maltempi, P.P., Avancini, R.M.P., Cytogenetics of the neotropical flesh fly Pattonella intermutans (Diptera, Sarcophagidae) (2000) Genet Mol Biol, 23, pp. 563-567
  • Parise-Maltempi, P.P., Avancini, R.M.P., C-banding and FISH in chromosomes of the blow flies Chrysomia megacephala and Chrysomia putoria (Diptera, Calliphoridae) (2001) Mem Inst Oswaldo Cruz, 96, pp. 371-377
  • Patrock, R.J.W., Porter, S.D., Gilbert, L.E., Folgarait, P.J., Distributional patterns of Pseudacteon associated with the Solenopsis saevissima complex in South America (2009) J Insect Sci, , in press
  • Porter, S.D., Biology and behavior of Pseudacteon decapitating flies (Diptera, Phoridae) that parasitize Solenopsis fire ants (Hymenoptera, Formicidae) (1998) Fla Entomol, 81, pp. 292-308
  • Porter, S.D., Pesquero, M.A., Illustrated key to Pseudacteon decapitating flies (Diptera, Phoridae) that attack Solenopsis saevissima complex fire ants in South America (2001) Fla Entomol, 90, pp. 135-138
  • Porter, S.D., Nogueira de Sá, L.A., Morrison, L.W., Establishment and dispersal of the fire ant decapitating fly Pseudacteon tricuspis in North Florida (2004) Biol Control, 29, pp. 179-188
  • Porter, S.D., Pesquero, M.A., Campiolo, S., Fowler, H.G., Growth and development of Pseudacteon phorid fly maggots (Diptera, Phoridae) in the head of Solenopsis fire ant workers (Hymenoptera, Formicidae) (1995) Environ Entomol, 24, pp. 475-479
  • Rafael, M.S., Tadei, W.P., Metaphase karyotypes of Anopheles (Nyssorhynchus) darlingi Root and A. (N.) nuneztovari Gabaldón (Diptera, Culicidae) (1998) Genet Mol Biol, 21, pp. 351-354
  • Rebagliati, P., Papeschi, A.G., Mola, L.M., Meiosis and fluorescent banding in Edessa meditabunda and E. rufomarginata (Heteroptera, Pentatomidae, Edessinae) (2003) Eur J Entomol, 100, pp. 11-18
  • Ross, K.G., Krieger, M.J.B., Keller, L., Shoemaker, D.D., Genetic variation and structure in native populations of the fire ant Solenopsis invicta: Evolutionary and demographic implications (2008) Biol J Linn Soc, 92, pp. 541-560
  • Sahara, K., Marec, F., TTAGG telomeric repeats in chromosomes of some insects and other arthropods (1999) Chromosome Res, 7, pp. 449-460. , and TrautW
  • Selivon, D., Perondini, A.L.P., Rocha, D., Karyotype characterization of Anastrepha fruit flies (Diptera, Tephritidae) (2005) Neotrop Entomol, 34, pp. 273-279
  • Solari, A.J., (1994) Sex chromosomes and sex determination in vertebrates, , CRC Press, Boca Raton, 336 pp
  • Steinemann, S., Retroelements: Tools for sex chromosome evolution (2005) Cytogenet Genome Res, 110, pp. 134-143. , and SteinemannM
  • Sumner, A.T., A simple technique for demonstrating centromeric heterochromatin (1972) Exp Cell Res, 75, pp. 304-306
  • Thead, L.G., Vogt, J.T., Streett, D.A., Dispersal of the fire ant decapitating fly, Pseudacteon curvatus (Diptera, Phoridae) in northeast Mississippi (2005) Fla Entomol, 88, pp. 214-216
  • Traut, W., Pachytene mapping in the female silkworm Bombyx mori L. (Lepidoptera) (1976) Chromosoma, 58, pp. 275-284
  • Sex determination in the fly Megaselia scalaris, a model system for primary steps of sex chromosome evolution (1994) Genetics, 136, pp. 1097-1104. , TrautW
  • Traut, W., Willhoeft, U., A jumping sex determining factor in the fly Megaselia scalaris (1990) Chromosoma, 99, pp. 407-412
  • Traut, W., Wollert, B., An X/Y DNA segment from an early stage of sex chromosome differentiation in the fly Megaselia scalaris (1998) Genome, 41, pp. 289-294
  • Traut, W., Sahara, K., Otto, T.D., Marec, F., Molecular differentiation of sex chromosomes probed by comparative genomic hybridization (1999) Chromosoma, 108, pp. 173-180
  • Tschinkel, W.R., Mikheyev, A.S., Storz, S.R., Allometry of workers of the fire ant, Solenopsis invicta (2003) J Insect Sci, 3, pp. e2
  • Wallace, A.J., Newton, M.E., Heterochromatin diversity and cyclic responses to selective silver staining in Aedes aegypti (L.) (1987) Chromosoma, 95, pp. 89-93
  • White, M.J.D., (1973) Animal Cytology and Evolution, , 3rd edition. Cambridge University Press, London, 961 pp
  • Willhoeft, U., Traut, W., Molecular differentiation of the homomorphic sex chromosomes in Megaselia scalaris (Diptera) detected by random DNA probes (1990) Chromosoma, 99, pp. 237-242
  • http://www.colostate.edu/Depts/Biology/MicroMeasure, MicroMeasure Web site, for MicroMeasure for Windows, version 3.3

Citas:

---------- APA ----------
Chirino, M.G., Folgarait, P.J., Gilbert, L.E., Lanzavecchia, S. & Papeschi, A.G. (2009) . Cytogenetic analysis of three species of Pseudacteon (Diptera, Phoridae) parasitoids of the fire ants using standard and molecular techniques. Genetics and Molecular Biology, 32(4), 740-747.
http://dx.doi.org/10.1590/S1415-47572009005000073
---------- CHICAGO ----------
Chirino, M.G., Folgarait, P.J., Gilbert, L.E., Lanzavecchia, S., Papeschi, A.G. "Cytogenetic analysis of three species of Pseudacteon (Diptera, Phoridae) parasitoids of the fire ants using standard and molecular techniques" . Genetics and Molecular Biology 32, no. 4 (2009) : 740-747.
http://dx.doi.org/10.1590/S1415-47572009005000073
---------- MLA ----------
Chirino, M.G., Folgarait, P.J., Gilbert, L.E., Lanzavecchia, S., Papeschi, A.G. "Cytogenetic analysis of three species of Pseudacteon (Diptera, Phoridae) parasitoids of the fire ants using standard and molecular techniques" . Genetics and Molecular Biology, vol. 32, no. 4, 2009, pp. 740-747.
http://dx.doi.org/10.1590/S1415-47572009005000073
---------- VANCOUVER ----------
Chirino, M.G., Folgarait, P.J., Gilbert, L.E., Lanzavecchia, S., Papeschi, A.G. Cytogenetic analysis of three species of Pseudacteon (Diptera, Phoridae) parasitoids of the fire ants using standard and molecular techniques. Genet. Mol. Biol. 2009;32(4):740-747.
http://dx.doi.org/10.1590/S1415-47572009005000073