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Abstract:

Allozyme and random amplified polymorphic DNA (RAPD) techniques have been compared for their usefulness for genetic and taxonomic studies in Prosopis glandulosa and P. velutina populations. Isozymes and RAPDs yielded similarly high estimates of genetic variability. Genetic structure and differentiation were analyzed through non-hierarchical Wright's FDT. For all populations considered, both markers produced low gene flow (Nm < 1) estimates. When only P. glandulosa populations were analyzed, isozyme data yielded higher gene flow estimates (Nm > 1), in agreement with that expected for conspecific populations. However, in RAPD data the expected reduction in FDT and the increase in Nm were not observed. Correlation between FDT and geographical distance matrices (Mantel test) for all populations was significant (P = 0.02) when based on isozymes, but not so (P = 0.33) when based on RAPDs. No significant associations among genetic and geographical or climatic variables were observed. Two isoenzyme systems (GOT and PRX) enabled us to distinguish between P. glandulosa and P. velutina, but no diagnostic band for recognition of populations or species studied here were detected by RAPD. However, RAPD markers showed higher values for genetic differentiation among conspecific populations of P. glandulosa and a lower coefficient of variation than those obtained from isozymes.

Registro:

Documento: Artículo
Título:Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae)
Autor:Bessega, C.; Saidman, B.O.; Vilardi, J.C.
Filiación:Dept. Ciencias Biológicas, Fac. Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:alcohol dehydrogenase; aminopeptidase; aspartate aminotransferase; esterase; isocitrate dehydrogenase; isoenzyme; peroxidase; plant DNA; superoxide dismutase; article; climate; controlled study; correlation function; data analysis; diagnostic value; gene frequency; gene structure; gene transfer; genetic analysis; genetic variability; geographic distribution; inheritance; legume; marker gene; nonhuman; random amplified polymorphic DNA; species difference; taxonomy; Prosopis glandulosa; Prosopis velutina
Año:2000
Volumen:23
Número:3
Página de inicio:639
Página de fin:648
DOI: http://dx.doi.org/10.1590/S1415-47572000000300024
Título revista:Genetics and Molecular Biology
Título revista abreviado:Genet. Mol. Biol.
ISSN:14154757
CODEN:GMBIF
CAS:alcohol dehydrogenase, 9031-72-5; aminopeptidase, 9031-94-1; aspartate aminotransferase, 9000-97-9; esterase, 9013-79-0; isocitrate dehydrogenase, 9001-58-5; peroxidase, 9003-99-0; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14154757_v23_n3_p639_Bessega

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Citas:

---------- APA ----------
Bessega, C., Saidman, B.O. & Vilardi, J.C. (2000) . Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae). Genetics and Molecular Biology, 23(3), 639-648.
http://dx.doi.org/10.1590/S1415-47572000000300024
---------- CHICAGO ----------
Bessega, C., Saidman, B.O., Vilardi, J.C. "Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae)" . Genetics and Molecular Biology 23, no. 3 (2000) : 639-648.
http://dx.doi.org/10.1590/S1415-47572000000300024
---------- MLA ----------
Bessega, C., Saidman, B.O., Vilardi, J.C. "Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae)" . Genetics and Molecular Biology, vol. 23, no. 3, 2000, pp. 639-648.
http://dx.doi.org/10.1590/S1415-47572000000300024
---------- VANCOUVER ----------
Bessega, C., Saidman, B.O., Vilardi, J.C. Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae). Genet. Mol. Biol. 2000;23(3):639-648.
http://dx.doi.org/10.1590/S1415-47572000000300024