Artículo

Rossi, L.F.; Luaces, J.P.; Browne, M.; Chirino, M.G.; Merani, M.S.; Mudry, M.D. "Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges" (2016) Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 796:40-45
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Abstract:

Sentinel species are useful tools for studying the deleterious effects of xenobiotics on wildlife. The large hairy armadillo (Chaetophractus villosus) is the most abundant and widely distributed mammal in Argentina. It is a long-lived, omnivorous, burrowing species, with fairly restricted home ranges. To evaluate the level of spontaneous genetic damage in this mammal, we determined the baseline values of several genotoxicity biomarkers. The study included 20 C. villosus adults of both sexes from eight pristine localities within its geographic distribution range. Genotoxicity analysis was performed on 72-h lymphocyte cultures, using mitomycin C as positive control. We obtained the baseline values of mitotic index (MI = 10.52 ± 0.30 metaphases/total cells, n = 20), chromosome aberrations (CA = 0.13 ± 0.22, n = 20), sister chromatid exchanges (SCE) = 6.55 ± 0.26, n = 6) and replication index (RI = 1.66, n = 6). MI and CA did not show significant differences (P > 0.05) among localities or between sexes. No significant differences in MI, CA, SCE, and RI (P > 0.05) were found between values from the pristine localities and historical data. There were significant differences in CA, SCE, and RI (P < 0.05) between lymphocyte cultures from pristine localities and those exposed to mitomycin C. We propose the large hairy armadillo as a sentinel organism for environmental biomonitoring of genotoxic chemicals due to its abundance, easy manipulation, well-known biology, the fact that it is usually exposed to different mixtures and concentrations of environmental contaminants, and the baseline values of genetic damage characterized by MI, CA, SCE and RI as biomarkers. © 2015 Elsevier B.V.

Registro:

Documento: Artículo
Título:Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges
Autor:Rossi, L.F.; Luaces, J.P.; Browne, M.; Chirino, M.G.; Merani, M.S.; Mudry, M.D.
Filiación:Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Piso 10 Lab. 6, Ciudad Autónoma de Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Grupo de Investigación en Biología Evolutiva (GIBE), Depto EGE, IEGEBA, Labs. 43 y 46- Piso 4- FCEyN- Pabellón II- Cdad, Universitaria. Intendente Güiraldes 2160 (C1428EGA), Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Biomarkers; Chaetophractus villosus; Chromosome aberrations; Genotoxicity; Sentinel organism; Sister chromatid exchange; biological marker; mitomycin; xenobiotic agent; mitomycin; xenobiotic agent; adult; animal cell; armadillo; Article; autosome; biological monitoring; cell proliferation; Chaetophractus villosus; chromosome aberration; controlled study; DNA damage; female; genetic damage; genotoxicity; karyotype; lymphocyte culture; male; mitosis index; nonhuman; priority journal; sex chromosome; sister chromatid; sister chromatid exchange; animal; chemically induced; chromosome aberration; drug effects; lymphocyte; metabolism; Xenarthra; Animals; Chromosome Aberrations; DNA Damage; Female; Lymphocytes; Male; Mitomycin; Mitotic Index; Sister Chromatid Exchange; Xenarthra; Xenobiotics
Año:2016
Volumen:796
Página de inicio:40
Página de fin:45
DOI: http://dx.doi.org/10.1016/j.mrgentox.2015.11.008
Título revista:Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Título revista abreviado:Mutat. Res. Genet. Toxicol. Environ. Mutagen.
ISSN:13835718
CODEN:MRGMF
CAS:mitomycin, 1404-00-8, 50-07-7, 74349-48-7; Mitomycin; Xenobiotics
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v796_n_p40_Rossi

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Citas:

---------- APA ----------
Rossi, L.F., Luaces, J.P., Browne, M., Chirino, M.G., Merani, M.S. & Mudry, M.D. (2016) . Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 796, 40-45.
http://dx.doi.org/10.1016/j.mrgentox.2015.11.008
---------- CHICAGO ----------
Rossi, L.F., Luaces, J.P., Browne, M., Chirino, M.G., Merani, M.S., Mudry, M.D. "Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges" . Mutation Research - Genetic Toxicology and Environmental Mutagenesis 796 (2016) : 40-45.
http://dx.doi.org/10.1016/j.mrgentox.2015.11.008
---------- MLA ----------
Rossi, L.F., Luaces, J.P., Browne, M., Chirino, M.G., Merani, M.S., Mudry, M.D. "Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges" . Mutation Research - Genetic Toxicology and Environmental Mutagenesis, vol. 796, 2016, pp. 40-45.
http://dx.doi.org/10.1016/j.mrgentox.2015.11.008
---------- VANCOUVER ----------
Rossi, L.F., Luaces, J.P., Browne, M., Chirino, M.G., Merani, M.S., Mudry, M.D. Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 2016;796:40-45.
http://dx.doi.org/10.1016/j.mrgentox.2015.11.008