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Abstract:

Microtubules (MT) are formed by the assembly of α- and β-tubulins and MT-associated proteins. We characterized the effects of pharmaceutical formulations containing the microtubule disruptors thiabendazole (TBZ) and griseofulvin (GF) on the mitotic machinery of plant (A. cepa) meristematic cells. GF concentrations between 10 and 250. μg/ml were tested. GF induced mitotic index inhibition and genotoxic effects, including chromosome fragments, bridges, lagged chromosomes, C-metaphases, tripolar cell division, disorganized anaphases and nuclear abnormalities in interphase cells. Efects on the mitotic machinery were studied by direct immunofluorescence with β-tubulin labeling and by DNA counterstaining with 4',6-diamidino-2-phenylindole (DAPI). Exposure of meristematic root cells to TBZ or GF, 100. μg/ml, caused microtubular damage which led to abnormal MT arrays. Our results suggest that GF induces abnormalities in spindle symmetry/polarity, while TBZ causes chromosome missegregation, polyploidy, and lack of cytokinesis. © 2014 Elsevier B.V.

Registro:

Documento: Artículo
Título:Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin
Autor:Andrioli, N.B.; Soloneski, S.; Larramendy, M.L.; Mudry, M.D.
Filiación:Grupo de Investigación en Biología Evolutiva (GIBE), Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires - IEGEBA (CONICET-UBA), Intendente Güiraldes 2160 - Ciudad Universitaria, C1428EGA Buenos Aires, Argentina
Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N 3, B1904AMA La Plata, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
Palabras clave:Allium cepa; Griseofulvin; Microtubule arrays; Thiabendazole; Tubulin; beta tubulin; griseofulvin; tiabendazole; anthelmintic agent; antifungal agent; griseofulvin; tiabendazole; anaphase; article; cell damage; cell division; chromosome aberration; chromosome analysis; controlled study; environmental exposure; immunofluorescence; micronucleus; microtubule; mini cell; mitosis index; mitosis spindle; nonhuman; nuclear buds; onion; polyploidy; priority journal; root cell; telophase; cytology; DNA damage; drug effects; genetics; meristem; metabolism; metaphase; microtubule; onion; plant cell; plant chromosome; Anthelmintics; Antifungal Agents; Chromosomes, Plant; DNA Damage; Griseofulvin; Meristem; Metaphase; Microtubules; Onions; Plant Cells; Polyploidy; Thiabendazole
Año:2014
Volumen:772
Página de inicio:1
Página de fin:5
DOI: http://dx.doi.org/10.1016/j.mrgentox.2014.06.009
Título revista:Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Título revista abreviado:Mutat. Res. Genet. Toxicol. Environ. Mutagen.
ISSN:13835718
CODEN:MRGMF
CAS:beta tubulin, 87090-36-6; griseofulvin, 126-07-8; tiabendazole, 148-79-8; Anthelmintics; Antifungal Agents; Griseofulvin; Thiabendazole
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v772_n_p1_Andrioli

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Citas:

---------- APA ----------
Andrioli, N.B., Soloneski, S., Larramendy, M.L. & Mudry, M.D. (2014) . Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 772, 1-5.
http://dx.doi.org/10.1016/j.mrgentox.2014.06.009
---------- CHICAGO ----------
Andrioli, N.B., Soloneski, S., Larramendy, M.L., Mudry, M.D. "Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin" . Mutation Research - Genetic Toxicology and Environmental Mutagenesis 772 (2014) : 1-5.
http://dx.doi.org/10.1016/j.mrgentox.2014.06.009
---------- MLA ----------
Andrioli, N.B., Soloneski, S., Larramendy, M.L., Mudry, M.D. "Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin" . Mutation Research - Genetic Toxicology and Environmental Mutagenesis, vol. 772, 2014, pp. 1-5.
http://dx.doi.org/10.1016/j.mrgentox.2014.06.009
---------- VANCOUVER ----------
Andrioli, N.B., Soloneski, S., Larramendy, M.L., Mudry, M.D. Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 2014;772:1-5.
http://dx.doi.org/10.1016/j.mrgentox.2014.06.009