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Abstract:

The genotoxicity of two nitroimidazole derivatives, ornidazole (ONZ) and metronidazole (MTZ) in the peripheral blood lymphocytes of Cebus libidinosus (CLI) (Primates, Cebidae) was assessed. Endpoints measured included sister chromatid exchange (SCE) frequency, cell proliferation kinetics (CPK), replication index (RI), mitotic index (MI), and damage incidence in or near CLI heterochromatin regions. MI and SCE values following ONZ or MTZ treatments were significantly different (p<0.001) from control. SCE frequency per chromosome was not proportional to chromosome length. The chromosomes most affected for SCE were 1, 2, 4, 6, 11-13, 17, and 18, many of which possess interstitial or terminal heterochromatin. In the CLI genome, chromosomes 11 and 17 showed higher susceptibility to damage RI was the only biomarker that did not show statistically significant differences between control and treated cultures. C. libidinosus bands 11q1.4 and 11q1.5 may be hot-spots in the context of nitroimidazole exposure. © 2011 Elsevier B.V.

Registro:

Documento: Artículo
Título:Biomarkers of genotoxicity and genomic instability in a non-human primate, Cebus libidinosus (Cebidae, Platyrrhini), exposed to nitroimidazole derivatives
Autor:Mudry, M.D.; Martinez, R.A.; Nieves, M.; Carballo, M.A.
Filiación:GIBE (Grupo de Investigación en Biología Evolutiva), Departamento de Ecología, Genética y Evolución (EGE), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), 1428EHA Buenos Aires, Argentina
CONICET, Argentina
Dpto. de Filosofia e Ciências Humanas, Universidade Estadual de Santa Cruz, Brazil
CIGETOX-Citogenética Humana y Genética Toxicológica, Departamento Bioquímica Clínica, INFIBIOC, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina
Junín 956 (1113), Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Cebus libidinosus; Genotoxicity; Heterochromatin; Nitroimidazoles; Sister chromatid exchange; metronidazole; ornidazole; animal cell; animal experiment; article; Cebus libidinosus; cell damage; cell division; cell proliferation; chromosome 11q; chromosome 12q; chromosome 13q; chromosome 17; chromosome 17q; chromosome 1p; chromosome 1q; chromosome 2q; chromosome 4q; chromosome 6q; chromosome 8q; chromosome banding pattern; chromosome size; controlled study; drug exposure; genetic marker; genomic instability; genotoxicity; heterochromatin; lymphocyte; mitosis; nonhuman; primate; priority journal; sister chromatid exchange; Animals; Biological Markers; Cebus; DNA Damage; Genomic Instability; Metronidazole; Mitotic Index; Mutagens; Ornidazole; Sister Chromatid Exchange; Cebidae; Cebus libidinosus; Platyrrhini; Primates
Año:2011
Volumen:721
Número:1
Página de inicio:108
Página de fin:113
DOI: http://dx.doi.org/10.1016/j.mrgentox.2011.01.002
Título revista:Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Título revista abreviado:Mutat. Res. Genet. Toxicol. Environ. Mutagen.
ISSN:13835718
CODEN:MRGMF
CAS:metronidazole, 39322-38-8, 443-48-1; ornidazole, 16773-42-5; Biological Markers; Metronidazole, 443-48-1; Mutagens; Ornidazole, 16773-42-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v721_n1_p108_Mudry

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Citas:

---------- APA ----------
Mudry, M.D., Martinez, R.A., Nieves, M. & Carballo, M.A. (2011) . Biomarkers of genotoxicity and genomic instability in a non-human primate, Cebus libidinosus (Cebidae, Platyrrhini), exposed to nitroimidazole derivatives. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 721(1), 108-113.
http://dx.doi.org/10.1016/j.mrgentox.2011.01.002
---------- CHICAGO ----------
Mudry, M.D., Martinez, R.A., Nieves, M., Carballo, M.A. "Biomarkers of genotoxicity and genomic instability in a non-human primate, Cebus libidinosus (Cebidae, Platyrrhini), exposed to nitroimidazole derivatives" . Mutation Research - Genetic Toxicology and Environmental Mutagenesis 721, no. 1 (2011) : 108-113.
http://dx.doi.org/10.1016/j.mrgentox.2011.01.002
---------- MLA ----------
Mudry, M.D., Martinez, R.A., Nieves, M., Carballo, M.A. "Biomarkers of genotoxicity and genomic instability in a non-human primate, Cebus libidinosus (Cebidae, Platyrrhini), exposed to nitroimidazole derivatives" . Mutation Research - Genetic Toxicology and Environmental Mutagenesis, vol. 721, no. 1, 2011, pp. 108-113.
http://dx.doi.org/10.1016/j.mrgentox.2011.01.002
---------- VANCOUVER ----------
Mudry, M.D., Martinez, R.A., Nieves, M., Carballo, M.A. Biomarkers of genotoxicity and genomic instability in a non-human primate, Cebus libidinosus (Cebidae, Platyrrhini), exposed to nitroimidazole derivatives. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 2011;721(1):108-113.
http://dx.doi.org/10.1016/j.mrgentox.2011.01.002