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Abstract:

Caiman latirostris is one of the two crocodilian species that inhabit Argentina. In this country, as a consequence of agricultural frontiers expansion during the last years, many areas of the geographic distribution of the broad snouted caiman overlap with regions of intensive agricultural activity. Contaminants released to the environment may induce genetic alterations in wildlife, which could lead to mutations and/or carcinogenesis. Up to the moment, no studies had been made concerning the possibbility to apply biomarkers of genotoxic evaluation in C. latirostris. The aim of this study was to adapt two widely used genotoxic techniques, the comet assay and the micronucleus test, for their application in C. latirostris and to determine the baseline values in this species, in order to establish its suitability as a sentinel organism for future genotoxic monitoring of environmental pollutants. A total of 41 juvenile caimans of 4 months old (FMO) and 10 months old (TMO) were used. Genotoxic techniques were applied on peripheral blood erythrocytes introducing the necessary modifications required by the material, which are presented here. Our results show that baseline values of DNA damage are quite stable among juvenile caimans (MN: FMO animals 0.87 ± 0.74 and TMO animals 1.04 ± 0.92; DI: FMO animals 103.40 ± 3.36 and TMO animals 120.08 ± 11.33), being independent of the nest of origin, sex and size of the animals and confirm the potential value of both short term tests as accurate screening tools for the evaluation of genotoxic agents in C. latirostris. This is the first reference to the application of genotoxic techniques on C. latirostris and the second in crocodilians. Data provided here will be useful for future studies involving the biomonitoring of natural regions where C. latirostris occurs, employing this species as a sentinel organism for genotoxic assessment of environmental pollutants. © 2007 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay
Autor:Poletta, G.L.; Larriera, A.; Kleinsorge, E.; Mudry, M.D.
Filiación:Proyecto Yacaré, (Min. Prod./MUPCN), A. del Valle 8700, CP: 3000 Santa Fe, Argentina
Cat. Manejo de Flora y Fauna, Fac. Hum. y Cs., UNL, Santa Fe, Argentina
Cát. de Toxicol. y Bioq. Legal, Fac. Bioq. y Cs. Biol., UNL, Santa Fe, Argentina
Grupo de Inv. en Biol. Evol.(GIBE), Fac. Cs. Exactas y Nat., UBA, Buenos Aires, Argentina
Palabras clave:Caiman latirostris; Comet assay; Genotoxicity; Micronuclei; Sentinel organism; article; Caiman latirostris; Chordata; comet assay; controlled study; genotoxicity; micronucleus; nonhuman; pollutant; pollution; priority journal; Alligators and Crocodiles; Animals; Comet Assay; DNA Damage; Environmental Monitoring; Female; Male; Micronucleus Tests; Animalia; Caiman; Caiman latirostris; Crocodylidae (all crocodiles)
Año:2008
Volumen:650
Número:2
Página de inicio:202
Página de fin:209
DOI: http://dx.doi.org/10.1016/j.mrgentox.2007.12.001
Título revista:Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Título revista abreviado:Mutat. Res. Genet. Toxicol. Environ. Mutagen.
ISSN:13835718
CODEN:MRGMF
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v650_n2_p202_Poletta

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Citas:

---------- APA ----------
Poletta, G.L., Larriera, A., Kleinsorge, E. & Mudry, M.D. (2008) . Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 650(2), 202-209.
http://dx.doi.org/10.1016/j.mrgentox.2007.12.001
---------- CHICAGO ----------
Poletta, G.L., Larriera, A., Kleinsorge, E., Mudry, M.D. "Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay" . Mutation Research - Genetic Toxicology and Environmental Mutagenesis 650, no. 2 (2008) : 202-209.
http://dx.doi.org/10.1016/j.mrgentox.2007.12.001
---------- MLA ----------
Poletta, G.L., Larriera, A., Kleinsorge, E., Mudry, M.D. "Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay" . Mutation Research - Genetic Toxicology and Environmental Mutagenesis, vol. 650, no. 2, 2008, pp. 202-209.
http://dx.doi.org/10.1016/j.mrgentox.2007.12.001
---------- VANCOUVER ----------
Poletta, G.L., Larriera, A., Kleinsorge, E., Mudry, M.D. Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 2008;650(2):202-209.
http://dx.doi.org/10.1016/j.mrgentox.2007.12.001