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Abstract:

Tetraploid Paspalum notatum (bahiagrass) is a valuable forage grass with aposporous apomictic reproduction. In a previous study, we showed that apospory in bahiagrass is under the control of a single dominant gene with a distorted segregation ratio. The objective of this work was to identify molecular markers linked to apospory in tetraploid P. notatum and establish a preliminary syntenic relationship with the genomic region associated with apospory in P. simplex. A F1 population of 290 individuals, segregating for apospory, was generated after crossing a completely sexual plant (Q4188) with a natural aposporous apomictic plant (Q4117). The whole progeny was classified as sexual or aposporous by embryo sacs analysis. A bulked segregant analysis was carried out to identify molecular markers co-segregating with apospory. Four hundred RAPD primers, 30 AFLP primers combinations and 85 RFLP clones were screened using DNA from both parental genotypes and aposporous and sexual bulks. Linkage analysis was performed with cytological and genetic information from the complete progeny. Cytoembryological analysis showed 219 sexual and 71 aposporous F1 individuals. Seven different molecular markers (2 RAPD, 4 AFLP and 1 RFLP) were found to be completely linked to apospory. The RFLP probe C1069, mapping to the telomeric region of the long arm of rice chromosome 12, was one of the molecular markers completely linked to apospory in P. notatum. This marker had been previously associated with apospory in P. simplex. A preliminary map of the chromosome region carrying the apospory locus was constructed.

Registro:

Documento: Artículo
Título:Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
Autor:Martínez, E.J.; Hopp, H.E.; Stein, J.; Ortiz, J.P.A.; Quarin, C.L.
Filiación:Inst. de Botanica del Nordeste, CONICET, CC209, (3400) Corrientes, Argentina
Instituto de Biotecnología, CNIA-INTA Castelar, CC25, (1712) Castelar, Buenos Aires, Argentina
Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Lab. de Fisiología Vegetal, Facultad de Ciencias Agrarias, CC14, (2123), Zavalla, Santa Fe, Argentina
Palabras clave:Apomixis; Apospory; Bulked segregant analysis; Comparative mapping; Molecular markers; Paspalum notatum; Embryophyta; Paspalum; Paspalum notatum; Paspalum simplex
Año:2003
Volumen:12
Número:4
Página de inicio:319
Página de fin:327
DOI: http://dx.doi.org/10.1023/B:MOLB.0000006868.12568.32
Título revista:Molecular Breeding
Título revista abreviado:Mol. Breed.
ISSN:13803743
CODEN:MOBRF
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13803743_v12_n4_p319_Martinez

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Citas:

---------- APA ----------
Martínez, E.J., Hopp, H.E., Stein, J., Ortiz, J.P.A. & Quarin, C.L. (2003) . Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers. Molecular Breeding, 12(4), 319-327.
http://dx.doi.org/10.1023/B:MOLB.0000006868.12568.32
---------- CHICAGO ----------
Martínez, E.J., Hopp, H.E., Stein, J., Ortiz, J.P.A., Quarin, C.L. "Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers" . Molecular Breeding 12, no. 4 (2003) : 319-327.
http://dx.doi.org/10.1023/B:MOLB.0000006868.12568.32
---------- MLA ----------
Martínez, E.J., Hopp, H.E., Stein, J., Ortiz, J.P.A., Quarin, C.L. "Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers" . Molecular Breeding, vol. 12, no. 4, 2003, pp. 319-327.
http://dx.doi.org/10.1023/B:MOLB.0000006868.12568.32
---------- VANCOUVER ----------
Martínez, E.J., Hopp, H.E., Stein, J., Ortiz, J.P.A., Quarin, C.L. Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers. Mol. Breed. 2003;12(4):319-327.
http://dx.doi.org/10.1023/B:MOLB.0000006868.12568.32