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Abstract:

The white-rot basidiomicete Trametes trogii (MYA 28-11) is an outstanding producer of laccase. A Doehlert experimental design was applied to optimize its lignocellulolytic enzyme production in solid-state fermentation. The impact on enzyme production of three quantitative variables, namely pH, copper and nitrogen concentrations, was investigated by using a wood-based solid medium supplemented with malt extract. Optimization was aimed at simultaneously minimizing cellulase activity and maximizing ligninolytic enzyme production. Such conditions were: pH 4.5, peptone 12.5 g l-1, and CuSO4 11 mM. Highest activities achieved were: laccase 901 and Mn-peroxidase 20 U g-1. There was not a direct correlation between the levels of enzymatic activities and the extent of losses of wood weight or components. The factorial approach also allowed us to quantify the enzyme activity in any part of the experimental domain, consequently, it was also possible to determine a culture medium to obtain crude extracellular extracts with high laccase (510 U g-1) and endoxylanase (780 U g-1) yields. These enzymes have gained renewed interest mainly due to their applications in paper industries for pulp treatment, improving the effectiveness of conventional bleaching. Considering the results obtained, T. trogii could be an attractive source of both enzymes. © 2007 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology
Autor:Levin, L.; Herrmann, C.; Papinutti, V.L.
Filiación:Micología Experimental, Dpto. de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, 1428 Ciudad Autonoma de Buenos Aires, Argentina
Palabras clave:Lignocellulolytic enzymes; Response surface methodology; Solid-state fermentation; White-rot fungi; Fermentation; Fungi; pH effects; Solid state reactions; Lignocellulolytic enzymes; Response surface methodology; Solid state fermentation; White-rot fungi; Enzyme activity; cellulase; copper; laccase; lignocellulose; manganese peroxidase; nitrogen; peptone; xylan endo 1,3 beta xylosidase; acidity; article; bleaching; correlation analysis; culture medium; enzyme activity; enzyme synthesis; experimental design; malt; nonhuman; paper industry; pH; priority journal; pulp processing; response surface method; solid state fermentation; Trametes; Trametes trogii; white rot fungus; wood; Funalia trogii; Fungi; Mya
Año:2008
Volumen:39
Número:1
Página de inicio:207
Página de fin:214
DOI: http://dx.doi.org/10.1016/j.bej.2007.09.004
Título revista:Biochemical Engineering Journal
Título revista abreviado:Biochem. Eng. J.
ISSN:1369703X
CODEN:BEJOF
CAS:cellulase, 9012-54-8; copper, 15158-11-9, 7440-50-8; laccase, 80498-15-3; lignocellulose, 11132-73-3; manganese peroxidase, 114995-15-2; nitrogen, 7727-37-9; xylan endo 1,3 beta xylosidase, 37278-89-0, 9025-55-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1369703X_v39_n1_p207_Levin

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Citas:

---------- APA ----------
Levin, L., Herrmann, C. & Papinutti, V.L. (2008) . Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology. Biochemical Engineering Journal, 39(1), 207-214.
http://dx.doi.org/10.1016/j.bej.2007.09.004
---------- CHICAGO ----------
Levin, L., Herrmann, C., Papinutti, V.L. "Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology" . Biochemical Engineering Journal 39, no. 1 (2008) : 207-214.
http://dx.doi.org/10.1016/j.bej.2007.09.004
---------- MLA ----------
Levin, L., Herrmann, C., Papinutti, V.L. "Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology" . Biochemical Engineering Journal, vol. 39, no. 1, 2008, pp. 207-214.
http://dx.doi.org/10.1016/j.bej.2007.09.004
---------- VANCOUVER ----------
Levin, L., Herrmann, C., Papinutti, V.L. Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology. Biochem. Eng. J. 2008;39(1):207-214.
http://dx.doi.org/10.1016/j.bej.2007.09.004