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Abstract:

Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a toxigenic fungus largely regarded as a single species by macroscopic and microscopic features. However, molecular studies have demonstrated that several morphologically identified A. fumigatus strains might be genetically distinct. This work was aimed to apply PCR-restriction length fragment polymorphisms (PCR-RFLP) and random amplification of polymorphic DNA (RAPD) molecular markers to characterize a set of feed-borne and clinical A. fumigatus sensu lato strains isolated from Argentina and Brazil and to determine and compare their genetic variability. All A. fumigatus strains had the same band profile and those typical of A. fumigatus sensu stricto positive controls by PCR-RFLP. Moreover, all Argentinian and Brazilian strains typified by RAPD showed similar band patterns to each other and to A. fumigatus sensu stricto reference strains regardless of their isolation source (animal feeds or human/animal clinical cases) and geographic origin. Genetic similarity coefficients ranged from 0.61 to 1.00, but almost all isolates showed 78% of genetic similarly suggesting that genetic variability was found at intraspecific level. Finally, benA sequencing confirmed its identification as A. fumigatus sensu stricto species. These results suggest that A. fumigatus sensu stricto is a predominant species into Aspergillus section Fumigati found in animal environments as well as in human/animal clinical cases, while other species may be rarely isolated. The strains involved in human and animal aspergillosis could come from the environment where this fungus is frequently found. Rural workers and animals would be constantly exposed. © 2015 The Author.

Registro:

Documento: Artículo
Título:Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
Autor:Pena, G.A.; Coelho, I.; Reynoso, M.M.; Soleiro, C.; Cavaglieri, L.R.
Filiación:Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km. 601, (5800) Río Cuarto, Córdoba, Argentina
Fellow of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, Buenos Aires, Argentina
Departamento de Microbiologia e Imunología Veterinária, Universidade Federal Rural Do Rio de Janeiro, Instituto de Veterinária, Rodovia BR 465 Km 7, Seropédica, Rio de Janeiro, Brazil
Conselho Nacional de Pesquisas Científicas (CNPq), Av. Antonio Carlos, 6627 Belo Horizonte, Brazil
Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917, Buenos Aires, Argentina
Palabras clave:animal feeds; feed-borne A. fumigatus; genetic variability estimation; molecular typing; molecular marker; Argentina; Article; Aspergillus fumigatus; Brazil; fungal biomass; fungal strain; genetic similarity; genetic variability; geographic origin; nonhuman; nucleotide sequence; polymerase chain reaction; random amplified polymorphic DNA; restriction fragment length polymorphism; animal; aspergillosis; Aspergillus fumigatus; classification; cluster analysis; DNA fingerprinting; DNA sequence; food control; genetic variation; genotype; human; isolation and purification; microbiological examination; microbiology; molecular epidemiology; molecular genetics; molecular typing; phylogeography; veterinary; Animals; Argentina; Aspergillosis; Aspergillus fumigatus; Brazil; Cluster Analysis; DNA Fingerprinting; Food Microbiology; Genetic Variation; Genotype; Humans; Molecular Epidemiology; Molecular Sequence Data; Molecular Typing; Mycological Typing Techniques; Phylogeography; Polymorphism, Restriction Fragment Length; Random Amplified Polymorphic DNA Technique; Sequence Analysis, DNA
Año:2015
Volumen:53
Número:7
Página de inicio:699
Página de fin:708
DOI: http://dx.doi.org/10.1093/mmy/myv040
Título revista:Medical Mycology
Título revista abreviado:Med. Mycol.
ISSN:13693786
CODEN:MEMYF
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13693786_v53_n7_p699_Pena

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Citas:

---------- APA ----------
Pena, G.A., Coelho, I., Reynoso, M.M., Soleiro, C. & Cavaglieri, L.R. (2015) . Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers. Medical Mycology, 53(7), 699-708.
http://dx.doi.org/10.1093/mmy/myv040
---------- CHICAGO ----------
Pena, G.A., Coelho, I., Reynoso, M.M., Soleiro, C., Cavaglieri, L.R. "Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers" . Medical Mycology 53, no. 7 (2015) : 699-708.
http://dx.doi.org/10.1093/mmy/myv040
---------- MLA ----------
Pena, G.A., Coelho, I., Reynoso, M.M., Soleiro, C., Cavaglieri, L.R. "Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers" . Medical Mycology, vol. 53, no. 7, 2015, pp. 699-708.
http://dx.doi.org/10.1093/mmy/myv040
---------- VANCOUVER ----------
Pena, G.A., Coelho, I., Reynoso, M.M., Soleiro, C., Cavaglieri, L.R. Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers. Med. Mycol. 2015;53(7):699-708.
http://dx.doi.org/10.1093/mmy/myv040