Abstract:
The aim of this work was to study the growth and production of ligninolytic enzymes by Fomes sclerodermeus using a natural medium based on wheat bran as the principal substrate in a solid-state fermentation. Growth was monitored by measuring the chitin content in the substrate. The maximum rate of growth was observed between days 7 and 18. A 38% total dry-weight loss of the substrate was measured after 28 days of cultivation. Differential hydrolysis of the substrate revealed that cellulose was more extensively degraded than lignin. In the 28-day incubation period, the losses of cellulose and lignin were 38 and 15%, respectively. No lignin peroxidase activity was found in any of the media tested. The maximum manganese-dependent peroxidase activity recorded was 6.3 U g-1 at 14 days, while the maximum laccase activity was 270 U g-1 at 28 days post-inoculation. Addition of commonly used inducers such as copper or manganese did not produce a further increase in the enzyme activities, nor did addition of glucose, asparagine, or malt extract.
Registro:
Documento: |
Artículo
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Título: | Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran |
Autor: | Papinutti, V.L.; Diorio, L.A.; Forchiassin, F. |
Filiación: | Lab. de Micología Exp., Depto. de Biodiversidad y Biol. Exp., Ciudad Universitaria Pab. II, 1428 Buenos Aires, Argentina
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Palabras clave: | Fermentation; Fomes sclerodermeus; Ligninases; Lignocellulosic; cellulose; fungal enzyme; laccase; lignin; lignin peroxidase; lignocellulose; manganese peroxidase; article; Basidiomycetes; enzyme activity; enzyme assay; enzyme substrate; fermentation; Fomes sclerodermeus; fungus culture; fungus growth; hydrolysis; nonhuman; nutrient; wheat bran; Basidiomycota; Fomes; Fungi; Triticum aestivum |
Año: | 2003
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Volumen: | 30
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Número: | 3
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Página de inicio: | 157
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Página de fin: | 160
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DOI: |
http://dx.doi.org/10.1007/s10295-003-0025-5 |
Título revista: | Journal of Industrial Microbiology and Biotechnology
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Título revista abreviado: | J. Ind. Microbiol. Biotechnol.
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ISSN: | 13675435
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CODEN: | JIMBF
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CAS: | cellulose, 61991-22-8, 68073-05-2, 9004-34-6; laccase, 80498-15-3; lignin, 9005-53-2; lignin peroxidase, 42613-30-9; lignocellulose, 11132-73-3; manganese peroxidase, 114995-15-2
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13675435_v30_n3_p157_Papinutti |
Referencias:
- Banci, L., Ciofi-Baffoni, S., Tien, M., Lignin and Mn peroxidase-catalyzed oxidation of phenolic lignin oligomers (1999) Biochemistry, 38, pp. 3205-3210
- Bourbonnais, R., Paice, M.G., Freiermuth, B., Bodie, E., Borneman, S., Reactivities of various mediators and laccases with kraft pulp and lignin model compounds (1997) Appl Environ Microbiol, 63, pp. 4627-4632
- Bourbonnais, R., Paice, M.G., Reid, I.D., Lanthier, P., Yaguchi, M., Lignin oxidation by laccase isozymes from Trametes versicolor and role of the mediator 2,2′-azino-bis (ethylbenzo-thiazoline-6-sulfonic acid) in kraft lignin depolimerization (1995) Appl Environ Microbiol, 61, pp. 1876-1880
- Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254
- Buswell, J.A., Cai, Y.J., Chang, Y., Effect of nutrient nitrogen and manganese on manganese peroxidase and laccase production by Lentinula (Lentinus) edodes (1995) FEMS Microbiol Lett, 128, pp. 81-88
- Galhaup, C., Wagner, H., Hinterstoisser, B., Haltrich, D., Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens (2002) Enzyme Microb Technol, 30, pp. 529-536
- Galliano, H., Gas, G., Seris, J.L., Boudet, A.M., Lignin degradation by Rigidoporus lignosus involves synergistic action of two oxidizing enzymes: Mn peroxidase and laccase (1991) Enzyme Microb Technol, 13, pp. 478-482
- Giardina, P., Palmieri, G., Scaloni, A., Fontanella, B., Faraco, V., Cennamo, G., Sannia, G., Protein and gene structure of a blue laccase from Pleurotus ostreatus (1999) Biochem J, 341, pp. 655-663
- Glenn, J.K., Gold, M.H., Purification and characterisation of an extracellular Mn(II)-dependent peroxidase from the lignin degrading basidiomycete Phanerochaete chrysosporium (1985) Arch Biochem Biophys, 242, pp. 329-341
- Hatakka, A., Lundell, T., Hatakka, A., Conversion of milled pine wood by manganese peroxidase from Phlebia radiata (2001) Appl Environ Microbiol, 67, pp. 4588-4593
- Johannes, C., Majcherczyk, A., Natural mediators in the oxidation of polycyclic aromatic hydrocarbons by laccase mediator systems (2000) Appl Environ Microbiol, 66, pp. 524-528
- Kanunfre, C.C., Zancan, G.T., Physiology of exolaccase production by Telephora terrestris (1998) FEMS Microbiol Lett, 16, pp. 151-156
- Kumaran, S., Sastry, C.A., Vikineswary, S., Laccase, cellulase and xylanase activities during growth of Pleurotus sajor-caju on sago hampas (1997) World J Microbiol Biotechnol, 13, pp. 43-49
- Levin, L., Forchiassin, F., Ramos, A.M., Copper induction of lignin modifying enzymes in the white-rot fungus Trametes trogii (2002) Mycologia, 94, pp. 377-383
- Magnelli, P., Forchiassin, F., Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates (1999) Mycologia, 91, pp. 359-364
- Mata, G., Savoie, J.M., Extracellular enzyme activities in six Lentinula edodes strains during cultivation in wheat straw (1998) World J Microb Biotechnol, 14, pp. 513-519
- Mester, T., Ambert-Balay, K., Ciofi-Baffoni, S., Banci, L., Jones, A.D., Tien, M., Oxidation of a tetrameric nonphenolic lignin model compound by lignin peroxidase (2001) J Biol Chem, 276, pp. 22985-22990
- Nelson, N.J., A photometric adaptation of the Somogyi method for the determination of glucose (1944) J Biochem, 153, pp. 375-380
- Papinutti, V.L., Forchiassin, F., Enzimas de hongos de pudrición blanca involucradas en la degradación de lignina (2000) Rev Arg Microbiol, 32, pp. 83-88
- Papinutti, V.L., Levin, L.N., Forchiassin, F., Decoloración y detoxificación del colorante Verde de Malaquita por Fomes sclerodermeus (2001) Abstracts of the 4th Meeting of the Society of Environmental Toxicology and Chemistry Latin America, , Buenos Aires
- Papinutti, V.L., Mouso, N., Forchiassin, F., Degradación de celulosa y lignina en madera de álamo por Fomes sclerodermeus (2001) Abstracts of the 9th Congreso Argentino de Microbiología, , Buenos Aires
- Peláez, F., Martínez, M.J., Martínez, A.T., Screening of 68 species of basidiomycetes for enzymes involved in lignin degradation (1995) Mycol Res, 99, pp. 37-42
- Plassard, C.S., Mousain, D.G., Salsac, L.E., Estimation of mycelial growth of basidiomycetes by means of chitin determination (1982) Phytochemistry, 21, pp. 345-348
- Ruttimann-Johnson, C., Cullen, D., Lamar, R.T., Manganese peroxidases of the white rot fungus Phanerochaete sordida (1994) Appl Environ Microbiol, 60, pp. 599-605
- (1983) Acid Insoluble Lignin in Wood and Pulp, , Tappi methods. T222 om-83
- Tekere, M., Zvauya, R., Read, J.S., Ligninolytic enzyme production in selected sub-tropical white rot fungi under different culture conditions (2001) J Basic Microbiol, 41, pp. 115-129
- Thurston, C.F., The structure and function of fungal laccases (1994) Microbiology, 140, pp. 19-26
- Tien, M., Kirk, T.K., Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium Burds (1983) Science, 221, pp. 661-663
- Wariishi, H., Valli, K., Gold, M.H., In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium (1991) Biochem Biophys Res Commun, 176, pp. 269-275
Citas:
---------- APA ----------
Papinutti, V.L., Diorio, L.A. & Forchiassin, F.
(2003)
. Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran. Journal of Industrial Microbiology and Biotechnology, 30(3), 157-160.
http://dx.doi.org/10.1007/s10295-003-0025-5---------- CHICAGO ----------
Papinutti, V.L., Diorio, L.A., Forchiassin, F.
"Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran"
. Journal of Industrial Microbiology and Biotechnology 30, no. 3
(2003) : 157-160.
http://dx.doi.org/10.1007/s10295-003-0025-5---------- MLA ----------
Papinutti, V.L., Diorio, L.A., Forchiassin, F.
"Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran"
. Journal of Industrial Microbiology and Biotechnology, vol. 30, no. 3, 2003, pp. 157-160.
http://dx.doi.org/10.1007/s10295-003-0025-5---------- VANCOUVER ----------
Papinutti, V.L., Diorio, L.A., Forchiassin, F. Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran. J. Ind. Microbiol. Biotechnol. 2003;30(3):157-160.
http://dx.doi.org/10.1007/s10295-003-0025-5