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Abstract:

A Box-Wilson central composite design was applied to optimize copper, veratryl alcohol and L-asparagine concentrations for Trametes trogii (BAFC 212) ligninolytic enzyme production in submerged fermentation. Decolorization of different dyes (xylidine, malachite green, and anthraquinone blue) by the ligninolytic fluids from the cultures was compared. The addition of copper stimulated laccase and glyoxal oxidase production, but this response was influenced by the medium N-concentration, with improvement higher at low N-levels. The medium that supported the highest ligninolytic production (22.75 U/ml laccase, 0.34 U/ml manganese peroxidase, and 0.20 U/ml glyoxal oxidase) also showed the greatest ability to decolorize the dyes. Only glyoxal oxidase activity limited biodecoloration efficiency, suggesting the involvement of peroxidases in the process. The addition of 1-hydroxybenzotriazole (a known laccase mediator) to the ligninolytic fluids increased both their range and rate of decolorization. The cell-free supernatant did not decolorize xylidine, poly R-478, azure B, and malachite green as efficiently as the whole broth, but results were similar in the case of indigo carmine and remazol brilliant blue R. This indicates that the mycelial biomass may supply other intracellular or mycelial-bound enzymes, or factors necessary for the catalytic cycle of the enzymes. It also implies that this fungus implements different strategies to degrade dyes with diverse chemical structures.

Registro:

Documento: Artículo
Título:Optimization of a culture medium for ligninolytic enzyme production and synthetic dye decolorization using response surface methodology
Autor:Trupkin, S.; Levin, L.; Forchiassin, F.; Viale, A.
Filiación:Micología Experimental, Universidad de Buenos Aires, Fac. de Cs. Exactas y Naturales, 1428 Capital Federal, Argentina
Palabras clave:Dye decolorization; Ligninolytic enzymes; Response surface methodology; Trametes trogii; White-rot fungi; anthraquinone; dye; glyoxal; indigo carmine; laccase; lignin; malachite green; manganese peroxidase; article; bacterium culture; cell free system; chemical structure; decolorization; enzyme active site; enzyme degradation; enzyme synthesis; fungal sclerotium; fungal strain; intermethod comparison; intracellular membrane; microbial biomass; nonhuman; response surface method; supernatant; Alcohol Oxidoreductases; Coloring Agents; Culture Media; Fermentation; Fungal Proteins; Industrial Microbiology; Laccase; Lignin; Polyporaceae; Bacteria (microorganisms); Funalia trogii; Fungi; Indigofera; Siproeta stelenes; Trametes
Año:2003
Volumen:30
Número:12
Página de inicio:682
Página de fin:690
DOI: http://dx.doi.org/10.1007/s10295-003-0099-0
Título revista:Journal of Industrial Microbiology and Biotechnology
Título revista abreviado:J. Ind. Microbiol. Biotechnol.
ISSN:13675435
CODEN:JIMBF
CAS:anthraquinone, 84-65-1; glyoxal, 107-22-2; indigo carmine, 860-22-0; laccase, 80498-15-3; lignin, 9005-53-2; malachite green, 569-64-2; manganese peroxidase, 114995-15-2; Alcohol Oxidoreductases, EC 1.1.-; Coloring Agents; Culture Media; Fungal Proteins; glyoxal oxidase, EC 1.1.3.-; Laccase, EC 1.10.3.2; Lignin, 9005-53-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13675435_v30_n12_p682_Trupkin

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Citas:

---------- APA ----------
Trupkin, S., Levin, L., Forchiassin, F. & Viale, A. (2003) . Optimization of a culture medium for ligninolytic enzyme production and synthetic dye decolorization using response surface methodology. Journal of Industrial Microbiology and Biotechnology, 30(12), 682-690.
http://dx.doi.org/10.1007/s10295-003-0099-0
---------- CHICAGO ----------
Trupkin, S., Levin, L., Forchiassin, F., Viale, A. "Optimization of a culture medium for ligninolytic enzyme production and synthetic dye decolorization using response surface methodology" . Journal of Industrial Microbiology and Biotechnology 30, no. 12 (2003) : 682-690.
http://dx.doi.org/10.1007/s10295-003-0099-0
---------- MLA ----------
Trupkin, S., Levin, L., Forchiassin, F., Viale, A. "Optimization of a culture medium for ligninolytic enzyme production and synthetic dye decolorization using response surface methodology" . Journal of Industrial Microbiology and Biotechnology, vol. 30, no. 12, 2003, pp. 682-690.
http://dx.doi.org/10.1007/s10295-003-0099-0
---------- VANCOUVER ----------
Trupkin, S., Levin, L., Forchiassin, F., Viale, A. Optimization of a culture medium for ligninolytic enzyme production and synthetic dye decolorization using response surface methodology. J. Ind. Microbiol. Biotechnol. 2003;30(12):682-690.
http://dx.doi.org/10.1007/s10295-003-0099-0