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Abstract:

Aims: To study the influence of peptides and proteolytic enzymes in the osmotic adaptation of Lactobacillus casei. Methods and Results: Di- and tri-peptides added individually increased the osmotolerance of Lact. casei when grown in a chemically defined medium (CDM) containing NaCl. Growth stimulation and the re-establishment in their presence of plasmid DNA supercoiling (recovery of the linking number) in hyperosmotic medium indicated that they are used as osmocompatible solutes as carnithine a known osmoprotector does. The investigation of the proteolytic system showed that in high osmolarity medium, the cell envelope-associated proteinase (PrtP), and PepX (X-prolyl-dipeptidyl aminopeptidase) increased activity and lost repression by peptides. PepI, an iminopeptidase was also derepressed. PepQ, a prolidase that specifically liberated proline from dipeptides, was almost unaffected. Derepression in the presence of peptides took place at the transcriptional level. However, the twofold activation of PrtP in CDM hyperosmotic medium was essentially through an increase of the apparent Vmax of the enzyme. Conclusions: These results strongly suggest a contribution of the proteolytic system peptide supply in the osmotic adaptation. Significance and Impact of the Study: Advances in understanding the role of peptides in the adaptation to high osmolarity particularly involved in dairy processes.

Registro:

Documento: Artículo
Título:Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes
Autor:Piuri, M.; Sanchez-Rivas, C.; Ruzal, S.M.
Filiación:Depto. de Quím. Biol., Fac. Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Depto. de Quím. Biol., Fac. Ciencias Exactas y Naturales, Pabellón II, 4 piso, 1428, Buenos Aires, Argentina
Palabras clave:Lactobacillus casei; Osmoregulation; Peptides; Proteolytic system; carnitine; peptide; proline dipeptidase; proline iminopeptidase; protein PepI; protein PepQ; proteinase; sodium chloride; unclassified drug; bacterium; osmoregulation; adaptation; article; bacterial growth; culture medium; DNA supercoiling; enzyme activity; gene repression; genetic transcription; Lactobacillus; Lactobacillus casei; nonhuman; osmoregulation; protein degradation; Culture Media; Food Microbiology; Genes, Bacterial; Humans; Lactobacillus casei; Osmolar Concentration; Peptide Hydrolases; Peptides; Reverse Transcriptase Polymerase Chain Reaction; Serine Endopeptidases; Sodium Chloride; Transcription, Genetic; Water-Electrolyte Balance; Bacteria (microorganisms); Lactobacillus; Lactobacillus casei; Posibacteria
Año:2003
Volumen:95
Número:2
Página de inicio:372
Página de fin:379
DOI: http://dx.doi.org/10.1046/j.1365-2672.2003.01971.x
Título revista:Journal of Applied Microbiology
Título revista abreviado:J. Appl. Microbiol.
ISSN:13645072
CODEN:JAMIF
CAS:carnitine, 461-06-3, 541-15-1, 56-99-5; proline dipeptidase, 9025-32-5; proline iminopeptidase, 9025-40-5; proteinase, 9001-92-7; sodium chloride, 7647-14-5; Culture Media; lactocepin, EC 3.4.21.96; Peptide Hydrolases, EC 3.4.-; Peptides; Serine Endopeptidases, EC 3.4.21.-; Sodium Chloride, 7647-14-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v95_n2_p372_Piuri

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Citas:

---------- APA ----------
Piuri, M., Sanchez-Rivas, C. & Ruzal, S.M. (2003) . Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes. Journal of Applied Microbiology, 95(2), 372-379.
http://dx.doi.org/10.1046/j.1365-2672.2003.01971.x
---------- CHICAGO ----------
Piuri, M., Sanchez-Rivas, C., Ruzal, S.M. "Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes" . Journal of Applied Microbiology 95, no. 2 (2003) : 372-379.
http://dx.doi.org/10.1046/j.1365-2672.2003.01971.x
---------- MLA ----------
Piuri, M., Sanchez-Rivas, C., Ruzal, S.M. "Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes" . Journal of Applied Microbiology, vol. 95, no. 2, 2003, pp. 372-379.
http://dx.doi.org/10.1046/j.1365-2672.2003.01971.x
---------- VANCOUVER ----------
Piuri, M., Sanchez-Rivas, C., Ruzal, S.M. Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes. J. Appl. Microbiol. 2003;95(2):372-379.
http://dx.doi.org/10.1046/j.1365-2672.2003.01971.x