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Guberman, A.; Hartmann, M.; Tiedtke, A.; Florin-Christensen, J.; Florin-Christensen, M. "A method for the preparation of Tetrahymena thermophila phospholipase A1 suitable for large-scale production" (1999) Journal of Applied Microbiology. 86(2):226-230
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Abstract:

A rapid and economical method for the purification of phospholipase A1 (PLA1) from the extracellular medium of the ciliate Tetrahymena thermophila is presented. Essentially, the procedure, here designated as purification by selective interaction (PSI), entails the incubation of media containing PLA1 with liposomes made of soy bean phospholipids. The PLA1-lipid complexes are precipitated by the addition of CaCl2 and collected by centrifugation. Elution of the PLA1 is effected by treating the complexes with 40% dimethylformamide, a reversible inhibitor of this enzyme, which is easily removed by dialysis. In combination with DEAE cellulose ion exchange chromatography, PSI yielded homogeneous PLA1 preparations with a 14% recovery and a 416-fold increase in specific activity. This procedure, which can be completed within 1 day, may prove useful for the isolation of phospholipases from other sources. This practical method for the purification of a microbial PLA1 opens the way to large-scale production of these types of enzyme, which are not as yet commercially available.

Registro:

Documento: Artículo
Título:A method for the preparation of Tetrahymena thermophila phospholipase A1 suitable for large-scale production
Autor:Guberman, A.; Hartmann, M.; Tiedtke, A.; Florin-Christensen, J.; Florin-Christensen, M.
Filiación:Institute of Neuroscience, Ciudad Universitaria, Buenos Aires, Argentina
Inst. für Allg. Zool./Genetik, Universität Münster, Münster, Germany
Dept. of Veterinary Microbiology, Washington State University, Pillman, WA 99164-7040, United States
Palabras clave:calcium chloride; diethylaminoethyl cellulose; liposome; n,n dimethylformamide; phospholipase a1; phospholipid; article; centrifugation; enzyme activity; enzyme purification; ion exchange chromatography; nonhuman; polyacrylamide gel electrophoresis; precipitation; tetrahymena thermophila; Animals; Culture Media; Liposomes; Phospholipases A; Tetrahymena thermophila; Ciliophora; Phaseolus (angiosperm); Protozoa; Tetrahymena thermophila
Año:1999
Volumen:86
Número:2
Página de inicio:226
Página de fin:230
DOI: http://dx.doi.org/10.1046/j.1365-2672.1999.00651.x
Título revista:Journal of Applied Microbiology
Título revista abreviado:J. Appl. Microbiol.
ISSN:13645072
CODEN:JAMIF
CAS:Culture Media; Liposomes; Phospholipases A, EC 3.1.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v86_n2_p226_Guberman

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Citas:

---------- APA ----------
Guberman, A., Hartmann, M., Tiedtke, A., Florin-Christensen, J. & Florin-Christensen, M. (1999) . A method for the preparation of Tetrahymena thermophila phospholipase A1 suitable for large-scale production. Journal of Applied Microbiology, 86(2), 226-230.
http://dx.doi.org/10.1046/j.1365-2672.1999.00651.x
---------- CHICAGO ----------
Guberman, A., Hartmann, M., Tiedtke, A., Florin-Christensen, J., Florin-Christensen, M. "A method for the preparation of Tetrahymena thermophila phospholipase A1 suitable for large-scale production" . Journal of Applied Microbiology 86, no. 2 (1999) : 226-230.
http://dx.doi.org/10.1046/j.1365-2672.1999.00651.x
---------- MLA ----------
Guberman, A., Hartmann, M., Tiedtke, A., Florin-Christensen, J., Florin-Christensen, M. "A method for the preparation of Tetrahymena thermophila phospholipase A1 suitable for large-scale production" . Journal of Applied Microbiology, vol. 86, no. 2, 1999, pp. 226-230.
http://dx.doi.org/10.1046/j.1365-2672.1999.00651.x
---------- VANCOUVER ----------
Guberman, A., Hartmann, M., Tiedtke, A., Florin-Christensen, J., Florin-Christensen, M. A method for the preparation of Tetrahymena thermophila phospholipase A1 suitable for large-scale production. J. Appl. Microbiol. 1999;86(2):226-230.
http://dx.doi.org/10.1046/j.1365-2672.1999.00651.x