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Abstract:

Aim: To evaluate the ability of probiotic Saccharomyces cerevisiae RC016 strain to reduce fumonisin B1 (FB1) in vitro and to optimize the culture conditions for the growth of the yeast employing surface response methodology. Methods and Results: Using Plackett-Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing (g l-1) fermentable sugars provided by sugar cane molasses (CMs), yeast extract (YE) and (NH4)2HPO4 (DAP) was formulated. The S. cerevisiae RC016 strain showed the greatest binding at all assayed FB1 concentration. The CMs, YE, DAP concentrations and incubation time influenced significantly the biomass of S. cerevisiae RC016. Conclusion: A combination of CMs 17%; YE 4·61 g l-1 and incubation time 60 h was optimum for maximum biomass of S. cerevisiae RC016. Significance and Impact of the Study: The importance of this work lies in the search for live strains with both probiotic and fumonisin B1 decontamination properties that could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources and would be included in a novel product to animal feed. © 2013 The Society for Applied Microbiology.

Registro:

Documento: Artículo
Título:Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1
Autor:Armando, M.R.; Galvagno, M.A.; Dogi, C.A.; Cerrutti, P.; Dalcero, A.M.; Cavaglieri, L.R.
Filiación:Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Agencia Córdoba Ciencia (ACC), Ciudad autónoma de Buenos Aires, Argentina
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Ciudad autónoma de Buenos Aires, Argentina
Departamento de Ingeniería Química, Pabellón de Industrias, Universidad de Buenos Aires, Ciudad autónoma de Buenos Aires, Argentina
Instituto de Investigaciones Biotecnológicas, CONICET, UNSAM, Buenos Aires, Argentina
Palabras clave:Biomass production; Cane molasses; Fumonisin B1; Saccharomyces cerevisiae; Statistical optimization; fumonisin B1; molasses; probiotic agent; bioassay; biomass; concentration (composition); fermentation; growth rate; optimization; probiotics; statistical analysis; toxic organism; yeast; article; batch fermentation; binding assay; biomass production; bioreactor; chemical structure; culture medium; fungal strain; fungus culture; fungus growth; in vitro study; incubation time; nonhuman; response surface method; Saccharomyces cerevisiae; sugarcane; Animal Feed; Biomass; Bioreactors; Carbon; Culture Media; Fermentation; Fumonisins; Industrial Microbiology; Models, Statistical; Molasses; Nitrogen; Probiotics; Saccharomyces cerevisiae; Saccharum; Animalia; Saccharomyces cerevisiae; Saccharum
Año:2013
Volumen:114
Número:5
Página de inicio:1338
Página de fin:1346
DOI: http://dx.doi.org/10.1111/jam.12144
Título revista:Journal of Applied Microbiology
Título revista abreviado:J. Appl. Microbiol.
ISSN:13645072
CODEN:JAMIF
CAS:fumonisin B1, 116355-83-0; molasses, 68476-78-8; Carbon, 7440-44-0; Culture Media; Fumonisins; Nitrogen, N762921K75; fumonisin B1, 116355-83-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v114_n5_p1338_Armando

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Citas:

---------- APA ----------
Armando, M.R., Galvagno, M.A., Dogi, C.A., Cerrutti, P., Dalcero, A.M. & Cavaglieri, L.R. (2013) . Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1. Journal of Applied Microbiology, 114(5), 1338-1346.
http://dx.doi.org/10.1111/jam.12144
---------- CHICAGO ----------
Armando, M.R., Galvagno, M.A., Dogi, C.A., Cerrutti, P., Dalcero, A.M., Cavaglieri, L.R. "Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1" . Journal of Applied Microbiology 114, no. 5 (2013) : 1338-1346.
http://dx.doi.org/10.1111/jam.12144
---------- MLA ----------
Armando, M.R., Galvagno, M.A., Dogi, C.A., Cerrutti, P., Dalcero, A.M., Cavaglieri, L.R. "Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1" . Journal of Applied Microbiology, vol. 114, no. 5, 2013, pp. 1338-1346.
http://dx.doi.org/10.1111/jam.12144
---------- VANCOUVER ----------
Armando, M.R., Galvagno, M.A., Dogi, C.A., Cerrutti, P., Dalcero, A.M., Cavaglieri, L.R. Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1. J. Appl. Microbiol. 2013;114(5):1338-1346.
http://dx.doi.org/10.1111/jam.12144