Abstract:
Aims: The aim of this study was to determine total fungal counts and the relative density of Aspergillus fumigatus and related species in silage samples intended for bovines before and after fermentation as well as to monitor the natural occurrence of gliotoxin in silage samples (pre- and postfermentation). Methods and methods: The survey was performed in farms located in São Paulo and Rio de Janeiro States in Brazil. In addition, the ability of A. fumigatus strains and related species strains to produce gliotoxin was also evaluated. A total of 300 samples were taken, immediately after opening of the silo (3-5 months) and during the ensiling period. Fungal counts were done by the surface-spread method. Gliotoxin production ability of isolates and natural contamination were determined by HPLC. Results: All postfermented samples had a total number of moulds exceeding 1 × 10 4 CFU g -1, with Aspergillus sp. as the most prevalent genus. Frequency of strains, among A. fumigatus and related species, was able to produce gliotoxin was similar in pre- and postfermented samples, except for sorghum, which showed differences between both kinds of samples. The highest toxin levels were produced by strains isolated from postfermented samples. More than 50% of the samples showed gliotoxin contamination levels that exceeded concentrations known to induce immunosuppressive and apoptotic effects in cells. Conclusions: The present data suggest that care should be taken because gliotoxin contamination in feedstuffs could affect productivity and also present a health risk for herds. Significance and Impact of the Study: Gliotoxin was found at quite important concentrations levels in pre- and postfermented substrates and its presence could therefore probably affect the productivity and health of herds. Current conservation and management practices do not avoid contamination with A. fumigatus on silage. Therefore, farm workers should be adequately protected during its handling. © 2012 The Authors. Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Registro:
Documento: |
Artículo
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Título: | Gliotoxin contamination in and pre- and postfermented corn, sorghum and wet brewer's grains silage in Sao Paulo and Rio de Janeiro State, Brazil |
Autor: | Keller, L.A.M.; Keller, K.M.; Monge, M.P.; Pereyra, C.M.; Alonso, V.A.; Cavaglieri, L.R.; Chiacchiera, S.M.; R Rosa, C.A. |
Filiación: | Departamento de Microbiologia e Imunología Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Seropédica, Rio de Janeiro, Brazil Conselho Nacional de Pesquisas Científicas (CNPq), Belo Horizonte, Brazil Departamento de Química, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina
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Idioma: |
Inglés
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Palabras clave: | Aspergillus fumigatus and related species; Fungi; Mycotoxin production; Silage; gliotoxin; agricultural worker; cattle; conservation management; fermentation; fungus; health risk; maize; management practice; silage; sorghum; substrate; toxin; apoptosis; article; Aspergillus fumigatus; Brazil; cattle farming; colony forming unit; cow; fermentation; food contamination; fungal strain; fungus isolation; grain; health hazard; health survey; herd; high performance liquid chromatography; maize; mould; nonhuman; silage; sorghum; toxin analysis; Animal Feed; Animals; Aspergillus fumigatus; Brazil; Cattle; Cereals; Colony Count, Microbial; Fermentation; Food Contamination; Gliotoxin; Silage; Sorghum; Zea mays; Brazil; Rio de Janeiro [Brazil]; Sao Paulo [Brazil]; Aspergillus fumigatus; Aspergillus sp.; Fungi; Zea mays |
Año: | 2012
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Volumen: | 112
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Número: | 5
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Página de inicio: | 865
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Página de fin: | 873
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DOI: |
http://dx.doi.org/10.1111/j.1365-2672.2012.05273.x |
Título revista: | Journal of Applied Microbiology
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Título revista abreviado: | J. Appl. Microbiol.
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ISSN: | 13645072
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CODEN: | JAMIF
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CAS: | gliotoxin, 37273-88-4, 67-99-2; Gliotoxin, 67-99-2
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v112_n5_p865_Keller |
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Citas:
---------- APA ----------
Keller, L.A.M., Keller, K.M., Monge, M.P., Pereyra, C.M., Alonso, V.A., Cavaglieri, L.R., Chiacchiera, S.M.,..., R Rosa, C.A.
(2012)
. Gliotoxin contamination in and pre- and postfermented corn, sorghum and wet brewer's grains silage in Sao Paulo and Rio de Janeiro State, Brazil. Journal of Applied Microbiology, 112(5), 865-873.
http://dx.doi.org/10.1111/j.1365-2672.2012.05273.x---------- CHICAGO ----------
Keller, L.A.M., Keller, K.M., Monge, M.P., Pereyra, C.M., Alonso, V.A., Cavaglieri, L.R., et al.
"Gliotoxin contamination in and pre- and postfermented corn, sorghum and wet brewer's grains silage in Sao Paulo and Rio de Janeiro State, Brazil"
. Journal of Applied Microbiology 112, no. 5
(2012) : 865-873.
http://dx.doi.org/10.1111/j.1365-2672.2012.05273.x---------- MLA ----------
Keller, L.A.M., Keller, K.M., Monge, M.P., Pereyra, C.M., Alonso, V.A., Cavaglieri, L.R., et al.
"Gliotoxin contamination in and pre- and postfermented corn, sorghum and wet brewer's grains silage in Sao Paulo and Rio de Janeiro State, Brazil"
. Journal of Applied Microbiology, vol. 112, no. 5, 2012, pp. 865-873.
http://dx.doi.org/10.1111/j.1365-2672.2012.05273.x---------- VANCOUVER ----------
Keller, L.A.M., Keller, K.M., Monge, M.P., Pereyra, C.M., Alonso, V.A., Cavaglieri, L.R., et al. Gliotoxin contamination in and pre- and postfermented corn, sorghum and wet brewer's grains silage in Sao Paulo and Rio de Janeiro State, Brazil. J. Appl. Microbiol. 2012;112(5):865-873.
http://dx.doi.org/10.1111/j.1365-2672.2012.05273.x