Abstract:
Aims: To study phosphate solubilization in Penicillium purpurogenum as function of medium pH, and carbon and nitrogen concentrations. Methods and Results: Tricalcium phosphate (CP) solubilization efficiency of P. purpurogenum was evaluated at acid or alkaline pH using different C and N sources. Glucose- and (NH4)2SO4-based media showed the highest P solubilization values followed by fructose. P. purpurogenum solubilizing ability was higher in cultures grown at pH 6·5 than cultures at pH 8·5. Organic acids were detected in both alkaline and neutral media, but the relative percentages of each organic acid differed. Highest P release coincided with the highest organic acids production peak, especially gluconic acid. When P. purpurogenum grew in alkaline media, the nature and concentration of organic acids changed at different N and C concentrations. A factorial categorical experimental design showed that the highest P-solubilizing activity, coinciding with the highest organic acid production, corresponded to the highest C concentration and lowest N concentration. Conclusions: The results described in the present study show that medium pH and carbon and nitrogen concentrations modulate the P solubilization efficiency of P. purpurogenum through the production of organic acids and particularly that of gluconic acid. In the P solubilization optimization studies, glucose and (NH4)2SO4 as C and N sources allowed a higher solubilization efficiency at high pH. Significance and Impact of the Study: This organism is a potentially proficient soil inoculant, especially in P-poor alkaline soils where other P solubilizers fail to release soluble P. Further work is necessary to elucidate whether these results can be extrapolated to natural soil ecosystems, where different pH values are present. Penicillium purpurogenum could be used to develop a bioprocess for the manufacture of phosphatic fertilizer with phosphate calcium minerals. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology.
Registro:
Documento: |
Artículo
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Título: | Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production |
Autor: | Scervino, J.M.; Papinutti, V.L.; Godoy, M.S.; Rodriguez, M.A.; Della Monica, I.; Recchi, M.; Pettinari, M.J.; Godeas, A.M. |
Filiación: | Dpto de Química Biológìca, FCEyN, Universidad de Buenos Aires, Buenos Aires, Argentina Dpto Biodiversidad y Biología Experimental, FCEyN, Universidad de Buenos Aires, Buenos Aires, Argentina
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Palabras clave: | Organic acids; Penicillium purpurogenum; Phosphate-solubilizing micro-organisms; Phosphorus; ammonium sulfate; calcium; carbon; carboxylic acid; fructose; gluconic acid; glucose; nitrogen; phosphate; alkalinity; calcium; carbon; concentration (composition); experimental design; fertilizer application; fungus; glucose; inoculation; nitrogen; optimization; organic acid; pH; phosphate; soil ecosystem; soil microorganism; solubilization; alkalinity; article; bioprocess; ecosystem; fungus culture; fungus growth; inoculation; nonhuman; Penicillium purpurogenum; pH; solubilization; Calcium Phosphates; Carbon; Culture Media; Gluconates; Hydrogen-Ion Concentration; Nitrogen; Penicillium; Soil Microbiology; Solubility; Penicillium purpurogenum |
Año: | 2011
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Volumen: | 110
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Número: | 5
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Página de inicio: | 1215
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Página de fin: | 1223
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DOI: |
http://dx.doi.org/10.1111/j.1365-2672.2011.04972.x |
Título revista: | Journal of Applied Microbiology
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Título revista abreviado: | J. Appl. Microbiol.
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ISSN: | 13645072
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CODEN: | JAMIF
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CAS: | ammonium sulfate, 7783-20-2; calcium, 14092-94-5, 7440-70-2; carbon, 7440-44-0; fructose, 30237-26-4, 57-48-7, 7660-25-5, 77907-44-9; gluconic acid, 133-42-6, 526-95-4, 66664-08-2; glucose, 50-99-7, 84778-64-3; nitrogen, 7727-37-9; phosphate, 14066-19-4, 14265-44-2; Calcium Phosphates; Carbon, 7440-44-0; Culture Media; Gluconates; Nitrogen, 7727-37-9; gluconic acid, 526-95-4; tricalcium phosphate, 7758-87-4
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v110_n5_p1215_Scervino |
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Citas:
---------- APA ----------
Scervino, J.M., Papinutti, V.L., Godoy, M.S., Rodriguez, M.A., Della Monica, I., Recchi, M., Pettinari, M.J.,..., Godeas, A.M.
(2011)
. Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production. Journal of Applied Microbiology, 110(5), 1215-1223.
http://dx.doi.org/10.1111/j.1365-2672.2011.04972.x---------- CHICAGO ----------
Scervino, J.M., Papinutti, V.L., Godoy, M.S., Rodriguez, M.A., Della Monica, I., Recchi, M., et al.
"Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production"
. Journal of Applied Microbiology 110, no. 5
(2011) : 1215-1223.
http://dx.doi.org/10.1111/j.1365-2672.2011.04972.x---------- MLA ----------
Scervino, J.M., Papinutti, V.L., Godoy, M.S., Rodriguez, M.A., Della Monica, I., Recchi, M., et al.
"Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production"
. Journal of Applied Microbiology, vol. 110, no. 5, 2011, pp. 1215-1223.
http://dx.doi.org/10.1111/j.1365-2672.2011.04972.x---------- VANCOUVER ----------
Scervino, J.M., Papinutti, V.L., Godoy, M.S., Rodriguez, M.A., Della Monica, I., Recchi, M., et al. Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production. J. Appl. Microbiol. 2011;110(5):1215-1223.
http://dx.doi.org/10.1111/j.1365-2672.2011.04972.x