Artículo

Miranda, P.V.; González-Echeverría, F.; Blaquier, J.A.; Mahuran, D.J.; Tezón, J.G. "Evidence for the participation of β-hexosaminidase in human sperm-zona pellucida interaction in vitro" (2000) Molecular Human Reproduction. 6(8):699-706
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Abstract:

Mammalian sperm-zona pellucida (ZP) interaction is mediated by sperm lectin-like proteins and ZP glycoproteins. We have previously reported the participation of binding sites for N-acetylglucosamine (GIcNAc) residues in human sperm function, including sperm interaction with the ZP. Additionally, previous results from our laboratory suggested that some of these events may be mediated by the glycosidase N-acetylglucosaminidase (β-hexosaminidase, Hex, in mammals). In this study, we report the possible participation of Hex in human sperm-ZP interaction. Human recombinant Hex (hrHex) was obtained by expression in a stable transfected CHO cell line. When the recombinant enzyme was present during hemizona (HZ) assays, the number of sperm bound per HZ was significantly reduced. The same result was obtained when HZ were preincubated with hrHex. Additionally, the presence of a Hex-specific substrate during the HZ assay produced the same inhibitory effect. These results suggest the participation of a sperm Hex in the interaction with human ZP in vitro.

Registro:

Documento: Artículo
Título:Evidence for the participation of β-hexosaminidase in human sperm-zona pellucida interaction in vitro
Autor:Miranda, P.V.; González-Echeverría, F.; Blaquier, J.A.; Mahuran, D.J.; Tezón, J.G.
Filiación:Instituto de Biología y Medicina Experimental (CONICET), Buenos Aires, Argentina
Fertilab, Buenos Aires, Argentina
Research Institute, Hospital for Sick Children, Toronto, Ont., Canada
Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Fertilization; Sperm-zona binding; Spermatozoa; Zona pellucida; β-Hexosaminidase; beta n acetylhexosaminidase; glycoprotein; glycosidase; article; binding affinity; binding site; CHO cell; electrophoresis; enzyme activation; fertilization in vitro; human; human cell; male; mammal; nonhuman; priority journal; protein binding; semen analysis; signal transduction; Western blotting; zona pellucida; Mammalia
Año:2000
Volumen:6
Número:8
Página de inicio:699
Página de fin:706
DOI: http://dx.doi.org/10.1093/molehr/6.8.699
Título revista:Molecular Human Reproduction
Título revista abreviado:Mol. Hum. Reprod.
ISSN:13609947
CODEN:MHREF
CAS:beta n acetylhexosaminidase, 37211-57-7, 9012-33-3, 9027-52-5; glycosidase, 9032-92-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13609947_v6_n8_p699_Miranda

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Citas:

---------- APA ----------
Miranda, P.V., González-Echeverría, F., Blaquier, J.A., Mahuran, D.J. & Tezón, J.G. (2000) . Evidence for the participation of β-hexosaminidase in human sperm-zona pellucida interaction in vitro. Molecular Human Reproduction, 6(8), 699-706.
http://dx.doi.org/10.1093/molehr/6.8.699
---------- CHICAGO ----------
Miranda, P.V., González-Echeverría, F., Blaquier, J.A., Mahuran, D.J., Tezón, J.G. "Evidence for the participation of β-hexosaminidase in human sperm-zona pellucida interaction in vitro" . Molecular Human Reproduction 6, no. 8 (2000) : 699-706.
http://dx.doi.org/10.1093/molehr/6.8.699
---------- MLA ----------
Miranda, P.V., González-Echeverría, F., Blaquier, J.A., Mahuran, D.J., Tezón, J.G. "Evidence for the participation of β-hexosaminidase in human sperm-zona pellucida interaction in vitro" . Molecular Human Reproduction, vol. 6, no. 8, 2000, pp. 699-706.
http://dx.doi.org/10.1093/molehr/6.8.699
---------- VANCOUVER ----------
Miranda, P.V., González-Echeverría, F., Blaquier, J.A., Mahuran, D.J., Tezón, J.G. Evidence for the participation of β-hexosaminidase in human sperm-zona pellucida interaction in vitro. Mol. Hum. Reprod. 2000;6(8):699-706.
http://dx.doi.org/10.1093/molehr/6.8.699