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Abstract:

Gamma-aminobutyric acid (GABA) concentrations in seminal plasma and washed spermatozoa from normal donors were assessed by a sensitive radioreceptor assay, and were detectable in both fractions. Specific binding of [3H]-muscimol was shown to be dependent on protein concentration, temperature and incubation time. [3H]-muscimol specific binding to human sperm membranes was significantly inhibited by the GABA type A receptor (GABA(A)) antagonist, bicuculline, and by the GABA(A) agonists, muscimol and isoguvacine, but not by the GABA type B receptor (GABA(B)) agonist baclofen. Scatchard analysis of [3H]-muscimol binding yielded a linear plot consistent with a single population of binding sites with a dissociation constant in the low nanomolar range. Incubation with GABA at a high micromolar concentration for 3 h under capacitating conditions resulted in an increase in the percentage of spermatozoa showing hyperactivated motility as assessed by computerized motility analyser. However, low micromolar concentrations of the GABA(A) agonist, muscimol, were sufficient to significantly increase sperm hyperactivity. These results suggest that the effect of GABA on human sperm motility might be mediated through a specific GABA(A) receptor.

Registro:

Documento: Artículo
Título:Occurrence of GABA and GABA receptors in human spermatozoa
Autor:Ritta, M.N.; Calamera, J.C.; Bas, D.E.
Filiación:Inst. de Biol. y Med. Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Lab. de Estud. en Reprod., Buenos Aires, Argentina
Palabras clave:GABA; Hyperactivated motility; Specific binding; Spermatozoa; 4 aminobutyric acid; 4 aminobutyric acid A receptor; 4 aminobutyric acid B receptor; 4 aminobutyric acid receptor; bicuculline; muscimol; article; controlled study; dissociation constant; human; human cell; male; normal human; priority journal; protein expression; receptor binding; receptor density; seminal plasma; spermatozoon; spermatozoon motility
Año:1998
Volumen:4
Número:8
Página de inicio:769
Página de fin:773
DOI: http://dx.doi.org/10.1093/molehr/4.8.769
Título revista:Molecular Human Reproduction
Título revista abreviado:Mol. Hum. Reprod.
ISSN:13609947
CODEN:MHREF
CAS:4 aminobutyric acid, 28805-76-7, 56-12-2; bicuculline, 485-49-4; muscimol, 2763-96-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13609947_v4_n8_p769_Ritta

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Citas:

---------- APA ----------
Ritta, M.N., Calamera, J.C. & Bas, D.E. (1998) . Occurrence of GABA and GABA receptors in human spermatozoa. Molecular Human Reproduction, 4(8), 769-773.
http://dx.doi.org/10.1093/molehr/4.8.769
---------- CHICAGO ----------
Ritta, M.N., Calamera, J.C., Bas, D.E. "Occurrence of GABA and GABA receptors in human spermatozoa" . Molecular Human Reproduction 4, no. 8 (1998) : 769-773.
http://dx.doi.org/10.1093/molehr/4.8.769
---------- MLA ----------
Ritta, M.N., Calamera, J.C., Bas, D.E. "Occurrence of GABA and GABA receptors in human spermatozoa" . Molecular Human Reproduction, vol. 4, no. 8, 1998, pp. 769-773.
http://dx.doi.org/10.1093/molehr/4.8.769
---------- VANCOUVER ----------
Ritta, M.N., Calamera, J.C., Bas, D.E. Occurrence of GABA and GABA receptors in human spermatozoa. Mol. Hum. Reprod. 1998;4(8):769-773.
http://dx.doi.org/10.1093/molehr/4.8.769