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Abstract:

Testicular protein Tpx-1, also known as CRISP-2, is a cysteine-rich secretory protein specifically expressed in the male reproductive tract. Since the information available on the human protein is limited to the identification and expression of its gene, in this work we have studied the presence and localization of human Tpx-1 (TPX1) in sperm, its fate after capacitation and acrosome reaction (AR), and its possible involvement in gamete interaction. Indirect immunofluorescence studies revealed the absence of significant staining in live or fixed non-permeabilized sperm, in contrast to a clear labelling in the acrosomal region of permeabilized sperm. These results, together with complementary evidence from protein extraction procedures strongly support that TPX1 would he mainly an intra-acrosomal protein in fresh sperm. After in vitro capacitation and ionophore-induced AR, TPX1 remained associated with the equatorial segment of the acrosome. The lack of differences in the electrophoretic mobility of TPX1 before and after capacitation and AR indicates that the protein would not undergo proteolytical modifications during these processes. The possible involvement of TPX1 in gamete interaction was evaluated by the hamster oocyte penetration test. The presence of anti-TPX1 during gamete co-incubation produced a significant and dose-dependent inhibition in the percentage of penetrated zona-free hamster oocytes without affecting sperm motility, the AR or sperm binding to the oolema. Together, these results indicate that human TPX1 would be a component of the sperm acrosome that remains associated with sperm after capacitation and AR, and is relevant for sperm-oocyte interaction. © European Society of Human Reproduction and Embryology 2005; all rights reserved.

Registro:

Documento: Artículo
Título:Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction
Autor:Busso, D.; Cohen, D.J.; Hayashi, M.; Kasahara, M.; Cuasnicú, P.S.
Filiación:Instituto de Biología y Medicina Experimental (IBYME), Buenos Aires 1428, Argentina
Department of Obstetrics and Gynaecology, Obihiro Kousei Hospital, Obihiro, Japan
Department of Pathology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
Palabras clave:Acrosome reaction; CRISP family; Gamete interaction; Sperm; Testes; cysteine; ionophore; secretory protein; testicular protein; unclassified drug; acrosome; acrosome reaction; adult; animal cell; article; binding kinetics; cell fate; cell interaction; cell labeling; controlled study; cytochemistry; electrophoretic mobility; female; gamete; hamster; human; human cell; immunofluorescence; in vitro study; incubation time; inhibition kinetics; male; male genital system; membrane permeability; nonhuman; oocyte; priority journal; protein analysis; protein degradation; protein expression; protein localization; protein modification; semen analysis; spermatozoon; spermatozoon capacitation; spermatozoon motility; spermatozoon penetration; zona pellucida; Antibodies; Glycoproteins; Humans; Male; Sperm Capacitation; Sperm-Ovum Interactions; Spermatozoa; Cricetinae
Año:2005
Volumen:11
Número:4
Página de inicio:299
Página de fin:305
DOI: http://dx.doi.org/10.1093/molehr/gah156
Título revista:Molecular Human Reproduction
Título revista abreviado:Mol. Hum. Reprod.
ISSN:13609947
CODEN:MHREF
CAS:cysteine, 4371-52-2, 52-89-1, 52-90-4; Antibodies; CRISP2 protein, human; Glycoproteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13609947_v11_n4_p299_Busso

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Citas:

---------- APA ----------
Busso, D., Cohen, D.J., Hayashi, M., Kasahara, M. & Cuasnicú, P.S. (2005) . Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction. Molecular Human Reproduction, 11(4), 299-305.
http://dx.doi.org/10.1093/molehr/gah156
---------- CHICAGO ----------
Busso, D., Cohen, D.J., Hayashi, M., Kasahara, M., Cuasnicú, P.S. "Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction" . Molecular Human Reproduction 11, no. 4 (2005) : 299-305.
http://dx.doi.org/10.1093/molehr/gah156
---------- MLA ----------
Busso, D., Cohen, D.J., Hayashi, M., Kasahara, M., Cuasnicú, P.S. "Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction" . Molecular Human Reproduction, vol. 11, no. 4, 2005, pp. 299-305.
http://dx.doi.org/10.1093/molehr/gah156
---------- VANCOUVER ----------
Busso, D., Cohen, D.J., Hayashi, M., Kasahara, M., Cuasnicú, P.S. Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction. Mol. Hum. Reprod. 2005;11(4):299-305.
http://dx.doi.org/10.1093/molehr/gah156